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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
#20652790 - 10/03/14 10:24 AM (9 years, 8 months ago) |
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Quote:
azur said:
Quote:
PussyFart said:
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azur said: I do know that you can see trich in lc also. But you cannot see yeast
Yea, but you're not him....he thinks he has microscopes for eyes.
For once i was agreeing with you pf
I know lol.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 6 months, 15 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: spacechildo]
#20652793 - 10/03/14 10:25 AM (9 years, 8 months ago) |
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The argument doesn't make sense to people who don't read the posts. I can't help you if you won't read.
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: Bumbaclotjohnson]
#20652802 - 10/03/14 10:27 AM (9 years, 8 months ago) |
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Quote:
Bumbaclotjohnson said: Is there a difference between LI and LC?
Look up slurry or slurries.
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: Bumbaclotjohnson]
#20653044 - 10/03/14 11:35 AM (9 years, 8 months ago) |
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Quote:
Bumbaclotjohnson said: So basically you blend up a cake in some clean water?
I blend up colonised agar.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: MudaFuka] 1
#20653585 - 10/03/14 02:09 PM (9 years, 8 months ago) |
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Of course you can't always see contams in an LC...that is pretty well accepted. Why would you go from spores -> LC -> agar when you could do spores -> agar -> LC and be assured your LC is clean from the beginning? Or just do what MudaFuka does and blend some colonized agar with sterilized water
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cronicr


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 5 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted]
#20653754 - 10/03/14 03:09 PM (9 years, 8 months ago) |
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enough guys, this is simply otto's tek the way he presented it, nothing wrong with friendly discussion but lets keep it friendly
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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shroobimon
nautilus pompilius

Registered: 09/07/14
Posts: 42
Last seen: 7 years, 20 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted]
#20653764 - 10/03/14 03:13 PM (9 years, 8 months ago) |
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wow, this is a great tek!!!
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: shroobimon]
#20653983 - 10/03/14 04:09 PM (9 years, 8 months ago) |
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Quote:
shroobimon said: wow, this is a great tek!!!
read the last few pages.
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wowimflabbergasted
supercalifragilistic



Registered: 07/16/12
Posts: 18,919
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
#20654492 - 10/03/14 06:03 PM (9 years, 8 months ago) |
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Quote:
cronicr said: enough guys, this is simply otto's tek the way he presented it, nothing wrong with friendly discussion but lets keep it friendly
I love how Otto presented this tek. Nothing but here.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: wowimflabbergasted]
#20654724 - 10/03/14 07:12 PM (9 years, 8 months ago) |
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otto! what up man its been a long while. how you been?
don't let all the haters knock lc's they fucking rock!
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
#20654832 - 10/03/14 07:50 PM (9 years, 8 months ago) |
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Quote:
eatyualive said: otto! what up man its been a long while. how you been?
don't let all the haters knock lc's they fucking rock!
QFFT
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
#20654946 - 10/03/14 08:16 PM (9 years, 8 months ago) |
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Quote:
bodhisatta said: Just click the first link in my signature figure out the pf tek make 5 grams of shrooms then worry about agar and lc
I've researched PF Tek quite a bit over the past week and I'd say I'm pretty confident I can follow the guides well enough to have success. The only monkey wrench I've encountered was apparently misinterpreting that I need to inject with LC/agar instead of just live spores.
I have enough for 20 1/2 pint jars and that's more than enough for me, even if a few will get contaminated. I'm going to do live spores and worry about agar/LC later. Thanks for the help.
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cronicr said: star it's only one cc to start a lc so you certainly can give it a shot
For sure. I ordered 2 syringes which IIRC are 10cc a piece. I'm going to inoculate with straight spores and save 1cc of each to mess with LC/agar later.
Just a quick question: I know I should store my syringes in the fridge for safe keeping. Are they at risk of contams if I store them in there AFTER I've used them to inject or is it fine?
I mean, can I inoculate, sterilize the needle with fire, and put them back in the fridge for later use?
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
#20656075 - 10/04/14 03:00 AM (9 years, 8 months ago) |
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Quote:
stareatclouds said:
Quote:
bodhisatta said: Just click the first link in my signature figure out the pf tek make 5 grams of shrooms then worry about agar and lc
I've researched PF Tek quite a bit over the past week and I'd say I'm pretty confident I can follow the guides well enough to have success. The only monkey wrench I've encountered was apparently misinterpreting that I need to inject with LC/agar instead of just live spores.
I have enough for 20 1/2 pint jars and that's more than enough for me, even if a few will get contaminated. I'm going to do live spores and worry about agar/LC later. Thanks for the help.
Quote:
cronicr said: star it's only one cc to start a lc so you certainly can give it a shot
For sure. I ordered 2 syringes which IIRC are 10cc a piece. I'm going to inoculate with straight spores and save 1cc of each to mess with LC/agar later.
Just a quick question: I know I should store my syringes in the fridge for safe keeping. Are they at risk of contams if I store them in there AFTER I've used them to inject or is it fine?
I mean, can I inoculate, sterilize the needle with fire, and put them back in the fridge for later use?
just to mention the pf tek was made for direct inoculation with spore syringes, its the standard with pf tek. http://www.mushroomvideos.com/BRF-Pf-Tek
and yes you can store them after use in a ziplock or whatever you want in your fridge just as long as they dont freeze. 
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A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
#20656081 - 10/04/14 03:06 AM (9 years, 8 months ago) |
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Thanks a lot. That's what I thought, but along the way, I think someone told me to go straight to LC which threw me off. Thanks for the help. I'll save some of the spores for LC at a later date, for sure.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
#20656427 - 10/04/14 07:47 AM (9 years, 8 months ago) |
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Quote:
stareatclouds said: Hi,
This thread is still ongoing despite the OP being very old. I was wondering if some of the more active posters who have read OP can confirm that the techniques involved are still relevant today.
Is this still a great guide to follow to make LC?
Thanks!
YES!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
#20656430 - 10/04/14 07:50 AM (9 years, 8 months ago) |
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Quote:
FilthyShroomz said: Roger rabbit was sayjng that you can just fill a colonized jar of grains with sterile water, shake it up and suck up the water with a syringe .. does that work lol ..sounds too ez
YES and he didn't invent that its a really old method.
http://www.shroomery.org/forums/showflat.php/Number/6817701#6817701
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#20656440 - 10/04/14 07:55 AM (9 years, 8 months ago) |
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Quote:
_OttO_ said: I'm grateful for the excited discussion this is prompting, thanks everyone for their opinions. It's interesting seeing statements that I agree, with juxtaposed with those I don't. I find it fascinating that the human mind works so hard to defend its perception of reality....
May I please re-state my points, I genuinely feel people miss them in their minds rush to defend their own perception of reality/reasons for believing something.
There's a few reasons why it always works -
1) Air movement creates a high spore movement and increases chances of contamination, hence why they call it a 'Still Air Box'. Shutting up the doors and windows and any drafts in your house an hour or two prior to working, sanitizing your work bench and hands before you start, not breathing on your work and working in slow, smooth motions (ALL normal mycology practices anyway and always good to incorporate) makes your house into an oversized Still Air Box anyway. I don't care how dirty your house was to start with, if you do this part properly your chances of failure are insignificant.
2) The jar is only opened a *crack* to introduce spores with a knife tip, or not opened at all if using a syringe - apart from exposing the inoculation hole. The means that not only is your outside environment really, really clean, but the media (LC) is *BARELY* even exposed to the 'open air' (or more appropriately termed 'external still air' and sanatised workspace!).
I have never suggested to work in a dirty environment.
3)This is the most important point that I feel is overlooked - this was meant to be appealing to people who are put off by the idea of achieving the level of sterility that you guys are insisting on, because it sounds too difficult.
It has been laid out in simple terms, and because the chances of success are very high, and it's a very easy method to follow requiring very little equipment that can be found very easily, that it would change the perception of people who think mycology is expensive or confusing.
Please stop missing this crucial point. That was my reason for writing it.
My request is simple - if you are one of the people who is claiming that doing an LC in a still room environment on a clean bench with gloved hands is reckless, I encourage you to actually force yourself to try it, in order to overcome your anxiety and unlearn that fear, and your argument holds no value unless it is from personal experience.
Quote:
Pastywhyte said:
I will say that it is possible that open air teks work. It is also possible for me to win the lottery.
Dichotomy unbalances this argument, reducing your credibility. If your chances of winning the lottery are 95+% then buy me a ticket....
The reality is this tek works, for the reasons stated above, almost every time, unless you don't follow it properly.
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Pastywhyte said: I like clean LC. I hate contamed LC. I would rather have LC that I know has the best chance to be clean, than have one that someone thinks has a good chance of being clean. I don't care if one is perceived to be easier than the other.
This however is a fairly balanced argument and one that has equal merit to mine. It doesn't take into account the very high success rate of using my method, properly. I feel without hesitation, from my own experience, that every LC I make on my kitchen bench has the BEST chance of being clean. This confidence comes from seeing the results, with 100's of liquid cultures made this way with no problems at all.
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Pastywhyte said: If anyone thinks agar is hard, then maybe they should try finger painting.
Classic...! 
Quote:
Asura said: I may be wrong here, but there seems to be 2 kinds of people here. Those that like the process and those that just want to get some mushrooms. I'm not in a hurry.
There are two types I'm noticing - those that think inside the box, and those that think out.
well said, and i couldn't have said it better myself! 5 stars to you otto!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: Pastywhyte]
#20656449 - 10/04/14 07:58 AM (9 years, 8 months ago) |
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Quote:
Pastywhyte said:
3) Coir can be used as a substrate, it was thought to be good for casing only
Today everyone grows on coir, no one would think to fruit in the dark and most common FC designs are more concerned with FAE than RH. No matter what, Open air inoculation of sterile media is a terrible idea. The end.
im not sure where this came from but ive been using coir in bulk subs since around 2001. its a really great addition to any bulk substrate. at first when folks started using coir. they were simply using it in casings as a replacement for peat moss that you have to ph balance. coir you don't. but, in the end everyone experimenting with it, started using it in subs and its a great mix. now more recently people started growing directly off of it. but, either way it was known long ago that coir kicks ass!
Edited by eatyualive (10/04/14 07:59 AM)
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azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
#20656533 - 10/04/14 08:24 AM (9 years, 8 months ago) |
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Anyone that doesn't know eatyualive, listen up and pay attention when he's talking. He's without doubt the best guy on this forum. He continues to experiment, write fantastic teks, reveals the history of other teks and all the while remains so very patient. Anything he says you can QFT!!!
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
#20656536 - 10/04/14 08:26 AM (9 years, 8 months ago) |
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i do all my spore inoculations open air. and flame away i welcome it but i don't ever sterilize my needles. i do use fresh sterile needles right out of the bag though.
do you know how many contams i have had using open air inoculations in pf jars or grain jars?
just about 0. i can maybe count on my hand one or two contams over the years and that was due to touching the needle on the edge of the jar as i was about to push the needle through the hole to inject solution.
http://www.shroomery.org/forums/showflat.php/Number/20300319
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