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InvisibleRahz
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Re: A *beginner friendly* method for making Liquid Culture [Re: shamanamba]
    #20381929 - 08/06/14 09:53 AM (9 years, 10 months ago)

I didn't say you were a clueless asshole. I'm not feeling the heat. Everyone get's their say. :shrug:


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Offlineshamanamba
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Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #20384214 - 08/06/14 06:17 PM (9 years, 10 months ago)

I wasn't accusing anyone of name calling. I wasn't trying to convey that I was butt hurt about anything lol Just being a sarcastic ass.

:datass:


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Invisiblestareatclouds
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Re: A *beginner friendly* method for making Liquid Culture [Re: shamanamba]
    #20644521 - 10/01/14 02:25 PM (9 years, 8 months ago)

Hi,

This thread is still ongoing despite the OP being very old. I was wondering if some of the more active posters who have read OP can confirm that the techniques involved are still relevant today.

Is this still a great guide to follow to make LC?

Thanks!

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OfflinecronicrFacebook
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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20644567 - 10/01/14 02:38 PM (9 years, 8 months ago)

i'm not a huge fan of it, i think lc's are just not needed for cubes and spores to lc doesn't' carry the success rate i'm after


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InvisibleFilthyShroomz
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #20647534 - 10/02/14 06:03 AM (9 years, 8 months ago)

If I have a pressure cooker,  do I need to boil the jar and lid at all? Do I just fill my jar with water and honey,  pc it,  then inoculate?


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.

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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647538 - 10/02/14 06:06 AM (9 years, 8 months ago)

Basically......but making an LC from spores is by far the worst way to start an LC.

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InvisibleFilthyShroomz
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20647606 - 10/02/14 06:31 AM (9 years, 8 months ago)

Why is that?? Should I not even think about making an LC at all until I finish my first project?  Is print to LC better?


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647608 - 10/02/14 06:33 AM (9 years, 8 months ago)

No print/syringe is ever 100% clean, as the cap was most likely fruited in open air.

LC is the perfect breeding ground for everything nasty.

If you are going to make an LC, for the best success rate, use a clean agar wedge.

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InvisibleFilthyShroomz
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20647613 - 10/02/14 06:35 AM (9 years, 8 months ago)

Ain't nothing wrong with using straight spore. Syringes right?  ( considering I can always do g2g with a couple master jars)  correct?


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InvisibleFilthyShroomz
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647617 - 10/02/14 06:38 AM (9 years, 8 months ago)

Roger rabbit was sayjng that you can just fill a colonized jar of grains with sterile water, shake it up and suck up the water with a syringe .. does that work lol ..sounds too ez


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OfflineBumbaclotjohnson
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647660 - 10/02/14 07:16 AM (9 years, 8 months ago)

Quote:

FilthyShroomz said:
Roger rabbit was sayjng that you can just fill a colonized jar of grains with sterile water, shake it up and suck up the water with a syringe .. does that work lol ..sounds too ez



It does work, but why not just take a spoonful off the top and drop that in another jar?


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Onlinetripdawg420
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Re: A *beginner friendly* method for making Liquid Culture [Re: Bumbaclotjohnson]
    #20647674 - 10/02/14 07:32 AM (9 years, 8 months ago)

yes glc works but theres alot involed  g2g from master to spawn bag is way less chance to come incontact with the boogie man:shrug:


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Invisible13shroomsM
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647853 - 10/02/14 08:49 AM (9 years, 8 months ago)

you guys need to stop answering with your negitive bias against LCs, if you dont like them fine but dont discourage..  LCs may not be the fool proof method for expanding myc but jeebus it works just fine if your sterile tek is good..  stop saying its crap and there are better ways.. 

answer the question asked and leave your extra opinions out unless asked... :shrug:

Im sure otto just loves all the bs you guys keep piling on his perfectly good thread..

my point is yes there are "better" or higher success rate beginner teks for all kinds of things like a flow hood is better than a glovebox or SAB BUT we dont tell others not to use them just how to do it correctly.  do you guys get the difference??  :wow:



Quote:

FilthyShroomz said:
Roger rabbit was sayjng that you can just fill a colonized jar of grains with sterile water, shake it up and suck up the water with a syringe .. does that work lol ..sounds too ez




yes like this...  http://www.shroomery.org/forums/showflat.php/Number/16071143

or old school GLC this way..  http://www.shroomery.org/forums/showflat.php/Number/6885786#6885786

its my prefered method of making an LC because I dont like to add sugar based anytihng to any of my myco projects.  water works fine but its not a food source like honey or LME with sugars/dextrose to feed and grow your myc.

I use the GLC to inoc grain jars to G2G later on..  with GLC you can know its clean by looking unlike standard LCs where you have to make a test jar.  :smirk:


:bonghit2:


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
    #20647870 - 10/02/14 08:56 AM (9 years, 8 months ago)

Quote:

13shrooms said:
you guys need to stop answering with your negitive bias against LCs, if you dont like them fine but dont discourage..  :



No one on this page was bias......cron said something about LCs not being needed for cubes, and the success rate is not right for him(and I agree, because the success rate is not that good when coming from spores)....

Then I said spores are by far the worst way to start an LC.....which is a fact.....I also said that making one from an agar wedge will give you the best chance of success....which is a fact.

What part of that was bias?

Quote:

13shrooms said:
my point is yes there are "better" or higher success rate beginner teks for all kinds of things like a flow hood is better than a glovebox or SAB BUT we dont tell others not to use them just how to do it correctly.  do you guys get the difference??  :wow:



A flow hood is not "Better" than a SAB....I have 100% success rate in my SAB....a flow hood would not improve that.....they are on the same level of effectiveness when used correctly.

Edited by PussyFart (10/02/14 08:58 AM)

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Invisible13shroomsM
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20647907 - 10/02/14 09:12 AM (9 years, 8 months ago)

quit justifying your bias against this tek again..  you can state facts when needed, nobody asked for random facts, your not answering the posters questions by stating acts..  if you dont like this method soo much stay out of the thread.  pretty eazy. :smirk:


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms] * 1
    #20647933 - 10/02/14 09:53 AM (9 years, 8 months ago)

Quote:

13shrooms said:
you can state facts when needed, nobody asked for random facts, your not answering the posters questions by stating facts.



Actually, I thought I was answering FilthyShroomz question......with relevant facts........but I guess not....I guess I should have just told him that LCs from spores are great, and there are no better methods......even tho that would be false.....

I guess I should have told him that every spore print/syringe is always guaranteed to be 100% clean and contam free...even tho that would be false............ :rolleyes:

U in a shitty mood or something? Everything I was talking about was relevant and true......

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InvisibleFilthyShroomz
starbuck

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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #20648395 - 10/02/14 12:00 PM (9 years, 8 months ago)

You guys both helped greatly.  Thanks !!


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InvisiblebodhisattaMDiscordReddit
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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20649031 - 10/02/14 02:36 PM (9 years, 8 months ago)

Besides all that since when are biases not allowed. When a user wants to express his opinion about a thread, tek, idea, etc.. it's going to be biased with their own experiences. that's why on average certain methods stand out, because on average more people have a positive bias for those methods because they fucking work well more often. :shrug: LC and GLC don't work as well as often, they're not noob friendly, and starting them with spores is risky even if it can work. I wouldn't suggest it to anyone just starting out  :2cents:

:bonghit2:

Let's all agree to never disagree about how to do things so that this place stays stagnant. :crazy2:

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Offlinespacechildo
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
    #20649491 - 10/02/14 04:19 PM (9 years, 8 months ago)

no matter how good your sterile techniques are you can't change what's already present on a print or in a syringe! :shrug:

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InvisiblePastywhyteMDiscord
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Re: A *beginner friendly* method for making Liquid Culture [Re: spacechildo]
    #20649549 - 10/02/14 04:32 PM (9 years, 8 months ago)

To be honest my biggest issue isn't even using spores to inoculate,  I have had bad luck with syringes but that's not to say everyone will. Its not even the open air inoculation, some people gave gotten lucky with open air g2g etc. No my problem is the unfiltered hole in the lid with nothing but some foil over it. If all we need for GE of any sterile media is a hole and some foil then why the hell do we bother with dry verm layers for pf tek or SFD's and polyfil for grain jar lids. Why would so many people have bothered to write teks on these things when all we need for 95% success is a hole and some foil :shrug:

Personally I think that anyone who advocates something like this had better use it themselves. Not used it once or twice and got away with it, they should be using it everytime cause its easier and if it works as well as they claim then making proper grain and LC lids would just be a waste of time and money.

I'm done. *hides thread*

Edited by Pastywhyte (10/02/14 05:04 PM)

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