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Offline_OttO_
Over Stimulated
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Registered: 06/01/05
Posts: 2,594
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Last seen: 12 days, 19 hours
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Re: A foolproof method for making Liquid culture [Re: 13shrooms]
    #16753062 - 08/29/12 08:49 AM (11 years, 9 months ago)

Quote:

AceofShroomz said:
Seems like this tek has a small element of luck as some are successful and some are not. Maybe the type of honey matters.




There defintely is more room for error with LC, but you can increase your chances according to your skill and care level. Picture yourself as a surgeon, and your bench top as an operating table. Close all doors and windows an hour before you work, sanitize your bench top and move slowly and things will work out at least 90% or more of the time.

Quote:

13shrooms said:
no, that "malt" isnt the same, hes talking about brewers malt (LME) or light malt extract used in agar mixes or brewing beer.  its just a better source of carbs than super sweet sugars like honey or karo. :thumbup:




My favourite is around about a 30/70 ratio of honey/pure maple syrup. Mycelium loves it, and it seems to stop that sediment issue from using just honey. I now use less than I originally wrote in the tek, about a teaspoon per 200ml of water. I also pressure cook now instead of steam/boil, but I used steaming in the write up because its looks more inviting to at least have a crack without having to buy anything. I will go back and edit the ratio's...

Personally I feel that LC is a wonderful way to play with fungus that appears easy and inviting enough to encourage more people to get into this hobby, and that was always the premise of this write up. However, of course agar is going to be more versatile and reliable if you compare them side by side. :mushroom2:

Edited by _OttO_ (08/29/12 09:07 AM)

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Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: A foolproof method for making Liquid culture [Re: _OttO_]
    #16753198 - 08/29/12 09:29 AM (11 years, 9 months ago)

I used grain LCs myself, never had much luck with spores in a regular LC. :shrug:

but in this hobby--- Whatever works and gets you the results you want is fine by me :yesnod:


:greenthumb::vato:


--------------------
:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
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OfflineStratosOZ
Noob


Registered: 05/19/12
Posts: 5
Last seen: 3 years, 11 months
Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
    #16780155 - 09/03/12 06:09 AM (11 years, 9 months ago)

Hey dude.

Noob question, since this is for noobs :tongue:.

Made mine exactly according to your tek, with a spore syringe of doubtful viability (P. cubensis).

At 1 day, there is this thick brownish sediment.  Here is a pic after shaking (hope you can see what I mean).


What do you reckon - is it normal sediment or contam?  Does contam happen this quickly?

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OfflineGoldenArrow
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Re: A *beginner friendly* method for making Liquid Culture [Re: StratosOZ]
    #16780185 - 09/03/12 06:22 AM (11 years, 9 months ago)

Caramelised honey maybe?

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OfflineStratosOZ
Noob


Registered: 05/19/12
Posts: 5
Last seen: 3 years, 11 months
Re: A *beginner friendly* method for making Liquid Culture [Re: GoldenArrow]
    #16780195 - 09/03/12 06:28 AM (11 years, 9 months ago)

Quote:

GoldenArrow said:
Caramelised honey maybe?



I hoped so too, but I made a "control" bottle with no inoculation - still looked pretty clear today

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Offlineshamanamba
The Shaman
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Registered: 06/15/05
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Re: A foolproof method for making Liquid culture [Re: _OttO_]
    #16780204 - 09/03/12 06:35 AM (11 years, 9 months ago)

Quote:

_OttO_ said:
of course agar is going to be more versatile and reliable if you compare them side by side. :mushroom2:






Love agar.

Never bothered dicking around with LC's once I realized how easy it is to prepare and work with agar.

Too many people speak of and treat agar work like it's some esoterically complex and difficult thing to do.

But it's not.

Especially some of the "no-pour" teks I've seen around here. They really bring agar to the noob in a way that's simple, affordable and easy.

I'm confident that if everyone here would realize that agar work is perfectly within the scope of even the most noobish of noobs, we'd rarely, if ever, even hear about LC's again.

I'm not bashing the people who use them, but I will preach against the use of LC's to my dieing day.

Yes, it works, but it's just not practical when compared to the superior results, reduced work load, and confidence in sterility that agar provides.


--------------------
The Shaman

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InvisibleJavadog
Continuing along
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Registered: 05/03/10
Posts: 7,385
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Re: A foolproof method for making Liquid culture [Re: shamanamba]
    #16832934 - 09/12/12 01:23 AM (11 years, 9 months ago)

I love the real maple syrup idea Otto. 

I am reminded of Carsten's suggestion that I use some wood tea when
making the dishes that I intended to get a wood lover's stubborn
spores to germinate on.

There must be nice "tree stuff" in that syrup.  (note the technical term ;0)

Take care,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineTrancedd
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #17134970 - 10/31/12 05:15 AM (11 years, 7 months ago)

Does anybody know if this works for Psilocybe semilanceata? They are growing in the UK right now so spores aren't an issue. Me and me and a friend want to try and inoculate as many fields/lawns as possible.

Few questions.

1. Does anybody know if it will work with Psilocybe semilanceata in this way?

Quote:

_OttO_ said:
Label with the date and strain etc. and incubate this in a dark place at 29C or 82F, giving it a gentle swirl once a day to encourage growth.

After a few days you will notice some small strands of mycelium forming, and it will be ready completely after about a week.





2. 29C seems warm. Is this required for all species to get the mycelium active or it species dependent? Right now room temperature here is about 15-22 C, and the semilanceata growing outdoors are growing in temperatures of roughly 9C. So what sort of temperature should I aim for?

3. Would, outdoors in optimum fruiting conditions be the best time to plant the mushrooms in the ground and do this?

Thanks

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Invisiblewolfedawwg
Veteran Lib Hunter & Gatherer
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Registered: 10/27/11
Posts: 893
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Re: A *beginner friendly* method for making Liquid Culture [Re: Trancedd]
    #17167912 - 11/05/12 06:38 PM (11 years, 7 months ago)

Pure un-pasteurized honey or regular pasteurized honey??
Also, can you pre-drill and sterilize the 1/2 pint lids and spore print directly to the underside of the lid (in a sterile environment of course), add H2O + LME and shake???


--------------------
:jah:Easy as Fuck Isopropyl Hash Oil:jah:


Psilocybe quebecensis


Edited by wolfedawwg (11/05/12 06:44 PM)

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Offlinezepski
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Re: A *beginner friendly* method for making Liquid Culture [Re: wolfedawwg]
    #17168487 - 11/05/12 07:52 PM (11 years, 7 months ago)

Shouldnt matter since you are gonna sterilize it once you make the mixture

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InvisibleRahz
Alive Again
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Registered: 11/10/05
Posts: 9,483
Re: A *beginner friendly* method for making Liquid Culture [Re: zepski]
    #17168727 - 11/05/12 08:22 PM (11 years, 7 months ago)

Printing to LC is tricky, and although I use LC almost exclusively I wouldn't suggest anything but agar for prints. Not that it can't work but there are more vectors for contams. Using an inoculation port for multi-spore, or a glovebox for clones (into an LC with inoculation port) is extremely safe in my experience provided the procedure is sound.

This tek is 7 years old... anyone who uses it, I would recommend investing in a bag of polyfill and tube of silicone. A piece of automotive vacuum tubing can also be useful for getting the filter up off the lid.



--------------------
rahz

comfort pleasure power love     truth awareness peace
I am      I feel      I do     I love  I speak    I see    I know


"A joyful heart is good medicine, but a crushed spirit dries up the bones." - Proverbs 17:22

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Invisibleshroomiin
ʂɧɽɸɵɱɨʝɳ


Registered: 12/24/07
Posts: 2,485
Loc: Zone 6 Flag
Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #17169638 - 11/05/12 10:48 PM (11 years, 7 months ago)

I love this tek. still usin' it.

Ive dropped spores open-air from a print into this LC with no contam's once used on substrate.


--------------------
ʋɳʛɼɑʈɛʄʋɭɖɛɑɗ

Code:
stdout ==> /dev/null


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InvisibleRahz
Alive Again
Male


Registered: 11/10/05
Posts: 9,483
Re: A *beginner friendly* method for making Liquid Culture [Re: shroomiin]
    #17169809 - 11/05/12 11:21 PM (11 years, 7 months ago)

If it didn't work this thread wouldn't be 30 pages long but time of year and location determine what's in the air so anything is possible including contams. Removing contam vectors seems like a good idea to me and the DIY route is cheap and easy.


--------------------
rahz

comfort pleasure power love     truth awareness peace
I am      I feel      I do     I love  I speak    I see    I know


"A joyful heart is good medicine, but a crushed spirit dries up the bones." - Proverbs 17:22

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InvisibleFischer
Teonanacatalyst
I'm a teapot

Registered: 06/03/10
Posts: 565
Loc: Downunder
Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #17238976 - 11/18/12 03:28 AM (11 years, 7 months ago)

I'v had dozens of LCs and inoculated jars of various species since I last had a contam, and I'm just working in the kitchen... 

No reason not to use LCs for inoculation, IMO.  Get an isolate to 'noc some LCs up with and you're set.

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InvisibleRahz
Alive Again
Male


Registered: 11/10/05
Posts: 9,483
Re: A *beginner friendly* method for making Liquid Culture [Re: Fischer]
    #17239605 - 11/18/12 09:33 AM (11 years, 7 months ago)

I didn't say not to use LCs, just suggesting an inoculation port and filter.


--------------------
rahz

comfort pleasure power love     truth awareness peace
I am      I feel      I do     I love  I speak    I see    I know


"A joyful heart is good medicine, but a crushed spirit dries up the bones." - Proverbs 17:22

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InvisibleFischer
Teonanacatalyst
I'm a teapot

Registered: 06/03/10
Posts: 565
Loc: Downunder
Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #17241247 - 11/18/12 02:48 PM (11 years, 7 months ago)

Nah dude, I wasn't responding to you, just the thread :wink:

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InvisibleMyco_Militia
Farmer
Registered: 11/08/12
Posts: 108
Loc: Flag
Re: A foolproof method for making Liquid culture [Re: shamanamba]
    #17241452 - 11/18/12 03:18 PM (11 years, 7 months ago)

Quote:

Javadog said:
I love the real maple syrup idea Otto. 

I am reminded of Carsten's suggestion that I use some wood tea when
making the dishes that I intended to get a wood lover's stubborn
spores to germinate on.

There must be nice "tree stuff" in that syrup.  (note the technical term ;0)

Take care,

JD



Quote:

shamanamba said:
Quote:

_OttO_ said:
of course agar is going to be more versatile and reliable if you compare them side by side. :mushroom2:






Love agar.

Never bothered dicking around with LC's once I realized how easy it is to prepare and work with agar.

Too many people speak of and treat agar work like it's some esoterically complex and difficult thing to do.

But it's not.

Especially some of the "no-pour" teks I've seen around here. They really bring agar to the noob in a way that's simple, affordable and easy.

I'm confident that if everyone here would realize that agar work is perfectly within the scope of even the most noobish of noobs, we'd rarely, if ever, even hear about LC's again.

I'm not bashing the people who use them, but I will preach against the use of LC's to my dieing day.

Yes, it works, but it's just not practical when compared to the superior results, reduced work load, and confidence in sterility that agar provides.




Can you elaborate on why agar provides more sterility?  I certainly have noticed it in my limited run of Gelatin/Malt vs Agar/Malt recipes.  Not statistically significant (3 runs), but still have noticed a difference in contamination rates.

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InvisibleMyco_Militia
Farmer
Registered: 11/08/12
Posts: 108
Loc: Flag
Re: A foolproof method for making Liquid culture [Re: Myco_Militia]
    #17261485 - 11/21/12 10:33 PM (11 years, 7 months ago)

Bump.  I'd like to see if someone has the answer to my question.

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InvisibleJavadog
Continuing along
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Registered: 05/03/10
Posts: 7,385
Loc: USA Flag
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Re: A foolproof method for making Liquid culture [Re: Myco_Militia]
    #17261995 - 11/22/12 12:08 AM (11 years, 7 months ago)

Agar does not provide sterility, but it does make it harder for
contams to hide.  They certainly can hide in LCs. 

I go from agar, to grains, and then G2G for volume when it is needed.

LCs are fine in my book.  I just prefer agar for certainty.

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: A foolproof method for making Liquid culture [Re: Javadog]
    #17262308 - 11/22/12 01:52 AM (11 years, 7 months ago)

Quote:

Javadog said:
Agar does not provide sterility, but it does make it harder for
contams to hide.  They certainly can hide in LCs. 

I go from agar, to grains, and then G2G for volume when it is needed.

LCs are fine in my book.  I just prefer agar for certainty.

Good luck,

JD




Same. :thumbup:

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