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Offlinezepski
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Re: A foolproof method for making Liquid culture [Re: Garrett91]
    #16511072 - 07/10/12 06:55 PM (11 years, 11 months ago)

If you used honey i bet its just sediment from the honey

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OfflineGarrett91
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Re: A foolproof method for making Liquid culture [Re: zepski]
    #16518081 - 07/12/12 02:23 AM (11 years, 11 months ago)

Okay well if its not mycelium and it is in fact sediment. I don't think there is any other type of growth taking place inside the jar... Is it possible that nothing has grown? Like I said previously I cant see any obvious contams.

Can nothing grow?


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Invisibleshroomiin
ʂɧɽɸɵɱɨʝɳ


Registered: 12/24/07
Posts: 2,485
Loc: Zone 6 Flag
Re: A foolproof method for making Liquid culture [Re: Garrett91]
    #16518254 - 07/12/12 05:00 AM (11 years, 11 months ago)

Just wanted to say I gave this honey lc a go and it is working wonders. beating a MEA based lc by a long shot.


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ʋɳʛɼɑʈɛʄʋɭɖɛɑɗ

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Offlinefatal222316
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Re: A foolproof method for making Liquid culture [Re: shroomiin]
    #16518673 - 07/12/12 08:35 AM (11 years, 11 months ago)

This is a great tek never done a LC I will start with this.  I had one question I am not doing a grow for a while and my spores are like 6 months old are they still useable and two can I make another LC from the old one to keep the culture going after it has run out of nutrients when and how would I do this, thanks for all the help very easy tek and will do it tonight with my pc thanks a lot. :wink:


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"Who says upright Ape's should know the secrets of the Universe"--Terrance McKenna

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Offlinefarming
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Re: A foolproof method for making Liquid culture [Re: fatal222316]
    #16674776 - 08/10/12 01:52 AM (11 years, 10 months ago)

what conditions should i store it in after the first 10 days


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the only mushrooms i grow are legal edible mushrooms such as shiitake and  portobello.

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InvisibleDoc_T
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Re: A foolproof method for making Liquid culture [Re: farming]
    #16674790 - 08/10/12 01:58 AM (11 years, 10 months ago)

Store it in the trash, LC sucks.
Choose a method that's likely to work. :thumbup:


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You make it all possible. Doesn't it feel good?

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Invisiblex CiVil x


Registered: 07/05/12
Posts: 634
Loc: Flying With Mother Nature
Re: A foolproof method for making Liquid culture [Re: Doc_T]
    #16675062 - 08/10/12 05:10 AM (11 years, 10 months ago)

Quote:

Doc_T said:
Store it in the trash, LC sucks.
Choose a method that's likely to work. :thumbup:



:yeahthatsfunny:


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“Empty your mind, be formless, shapeless - like water. Now you put water into a cup, it becomes the cup, you put water into a bottle, it becomes the bottle, you put it in a teapot, it becomes the teapot. Now water can flow or it can crash. Be water, my friend.” Bruce Lee

myco-tek.org

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Offline_OttO_
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Re: A foolproof method for making Liquid culture [Re: x CiVil x]
    #16675842 - 08/10/12 10:47 AM (11 years, 10 months ago)

Doc_t, the rumours are true!

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Offlinefarming
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Registered: 06/27/12 Happy 12th Shroomiversary!
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Re: A foolproof method for making Liquid culture [Re: Doc_T]
    #16675991 - 08/10/12 11:23 AM (11 years, 10 months ago)

Quote:

Doc_T said:
Store it in the trash, LC sucks.
Choose a method that's likely to work. :thumbup:




ok there johny rainclouds lol, atleast i see your not only a negative nancy to me lol


but really can someone tell me where i should store it after the first 10 days


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the only mushrooms i grow are legal edible mushrooms such as shiitake and  portobello.

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Offlinezepski
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Re: A foolproof method for making Liquid culture [Re: farming]
    #16676029 - 08/10/12 11:33 AM (11 years, 10 months ago)

Store it in the fridge if you want it to slow/stop growing.  Or you can just store it on shelf with your other jars and let it grow as much as it can.  But since you are asking im assuming you want the fridge. 

Also i would knock up a jar or 3 to test the LC.

Also Doc_T has a point LC are a gamble since you don't know if they are good till they are tested.  GLC (Grain Liquid Culture) is a better way to go if you must do LC.  However G2G cant be beat for speed :P

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Offlinefarming
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Registered: 06/27/12 Happy 12th Shroomiversary!
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Re: A foolproof method for making Liquid culture [Re: zepski]
    #16676175 - 08/10/12 12:02 PM (11 years, 10 months ago)

Quote:

zepski said:
Store it in the fridge if you want it to slow/stop growing.  Or you can just store it on shelf with your other jars and let it grow as much as it can.  But since you are asking im assuming you want the fridge. 

Also i would knock up a jar or 3 to test the LC.

Also Doc_T has a point LC are a gamble since you don't know if they are good till they are tested.  GLC (Grain Liquid Culture) is a better way to go if you must do LC.  However G2G cant be beat for speed :P





thnaks for the info, tongiht im going to knock up some rye berries with a piece of cake and i figure since its jusst honey and water i might as well give it a shot with with another piece of cake, once the grain is colonized il be doing the GLC, i figure i should try to try everyhting atleast once


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the only mushrooms i grow are legal edible mushrooms such as shiitake and  portobello.

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InvisibleDoc_T
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Re: A foolproof method for making Liquid culture [Re: farming]
    #16676236 - 08/10/12 12:15 PM (11 years, 10 months ago)

No point in going LC->grain->GLC. Think about what you're doing there.


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You make it all possible. Doesn't it feel good?

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Offlinefarming
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Registered: 06/27/12 Happy 12th Shroomiversary!
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Re: A foolproof method for making Liquid culture [Re: Doc_T]
    #16676323 - 08/10/12 12:28 PM (11 years, 10 months ago)

Quote:

Doc_T said:
No point in going LC->grain->GLC. Think about what you're doing there.




im going cake to grain to GLC

also cake to LC just to experiment


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the only mushrooms i grow are legal edible mushrooms such as shiitake and  portobello.

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InvisibleDoc_T
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Re: A foolproof method for making Liquid culture [Re: farming]
    #16676391 - 08/10/12 12:37 PM (11 years, 10 months ago)

Quote:

cake to grain to GLC




Might work, but it's the same exact procedure to take a cake sample to agar as to put it into grains.
From the agar you could then grow it on grains for GLC, or just drop an agar wedge into a malt LC jar.
LC from agar is way less sketchy than LC from spores.

Quote:

cake to LC




Again, might work. Somewhat less likely than cake->grain, imho.

But all of this cake slicing is good practice for tissue cloning.
People sometimes use LC for cloning. I never did get that to work, but it's cheap to try.
Tissue->grain has worked for me, and of course agar is the standard method.
So whether it works or not it's probably useful, but please understand the risk and the the LCs.


--------------------
You make it all possible. Doesn't it feel good?

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OfflineAceofShroomz
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Re: A foolproof method for making Liquid culture [Re: Doc_T]
    #16676871 - 08/10/12 02:30 PM (11 years, 10 months ago)

Quote:

Doc_T said:LC from agar is way less sketchy than LC from spores.



Yup I tried this tek from a known working spore syringe with no success. It looked alright. Whispy white mycelium in the water. But when I tried to 'noc up a jar with it.. No success. It didn't contam.. it didn't grow anything. Seems like this tek has a small element of luck as some are successful and some are not. Maybe the type of honey matters.


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"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.

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Offlinefarming
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Registered: 06/27/12 Happy 12th Shroomiversary!
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Re: A foolproof method for making Liquid culture [Re: AceofShroomz]
    #16676923 - 08/10/12 02:40 PM (11 years, 10 months ago)

Quote:

AceofShroomz said:
Quote:

Doc_T said:LC from agar is way less sketchy than LC from spores.



Yup I tried this tek from a known working spore syringe with no success. It looked alright. Whispy white mycelium in the water. But when I tried to 'noc up a jar with it.. No success. It didn't contam.. it didn't grow anything. Seems like this tek has a small element of luck as some are successful and some are not. Maybe the type of honey matters.



thats a good point im trying to see in the pic what kind of honey it is


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the only mushrooms i grow are legal edible mushrooms such as shiitake and  portobello.

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InvisibleDoc_T
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Re: A foolproof method for making Liquid culture [Re: farming]
    #16676933 - 08/10/12 02:42 PM (11 years, 10 months ago)

Get the kind of honey that comes in a plastic bear.

But malt is better. Plus if you have malt, you can make agar as well as LC. :thumbup:


--------------------
You make it all possible. Doesn't it feel good?

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OfflineAceofShroomz
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Re: A foolproof method for making Liquid culture [Re: Doc_T]
    #16676974 - 08/10/12 02:49 PM (11 years, 10 months ago)

Quote:

Doc_T said:
Get the kind of honey that comes in a plastic bear.

But malt is better. Plus if you have malt, you can make agar as well as LC. :thumbup:



Should've got some malt! Will do next time. I'm ready to try another LC. Just made my first syringes from my own spore print. Will know in a couple days if it was successful. I have no flowhood or glovebox so it's a little tricky everything I do I have to be extra careful. I 'noc up jars and do work over my oven with the door open lol. Works really well so far.


--------------------
"We are the artists, painting on the blank canvas of Reality." ~Me
The Mushroom Speaks

All information & pictures I post are falsified for entertainment purposes only.

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InvisibleRRedBBeard
SE Connecticut
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Re: A foolproof method for making Liquid culture [Re: AceofShroomz]
    #16752679 - 08/29/12 06:14 AM (11 years, 9 months ago)

Quote:

AceofShroomz said:
Quote:

Doc_T said:
Get the kind of honey that comes in a plastic bear.

But malt is better. Plus if you have malt, you can make agar as well as LC. :thumbup:



Should've got some malt! Will do next time. I'm ready to try another LC. Just made my first syringes from my own spore print. Will know in a couple days if it was successful. I have no flowhood or glovebox so it's a little tricky everything I do I have to be extra careful. I 'noc up jars and do work over my oven with the door open lol. Works really well so far.




I have the type of malt that you would put in a milkshake, and its just malt, no dairy. Can I use this successfully?

Also, I have the same sterilizing challenges you have --no flow hood, etc. When I'm noc'ing a bag, I steam up the bathroom really well since tams are much less likely in that heavy atmosphere. For injecting individual jars, I take a fresh clear produce bag from the local market, add a cotton ball soaked in alcohol or weak bleach and use that as a mini glove box. Pre-flame your needle if you do this with alcohol of course! I tried making an lc from a 3 month old portabello syringe with no luck, but no tams either, not sure why the spores did nothing...

--Rr


--------------------
The only difference between a rut and
a grave is the depth of the hole.

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Invisible13shroomsM
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Re: A foolproof method for making Liquid culture [Re: RRedBBeard]
    #16752698 - 08/29/12 06:27 AM (11 years, 9 months ago)

no, that "malt" isnt the same, hes talking about brewers malt (LME) or light malt extract used in agar mixes or brewing beer.  its just a better source of carbs than super sweet sugars like honey or karo. :thumbup:


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