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Offlineluisfer420
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Re: A *beginner friendly* method for making Liquid Culture [Re: luisfer420]
    #15806187 - 02/13/12 11:09 PM (12 years, 4 months ago)



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Offlineluisfer420
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Re: A *beginner friendly* method for making Liquid Culture [Re: luisfer420]
    #15806188 - 02/13/12 11:09 PM (12 years, 4 months ago)

hey people!! well i made an LC and inoculated 8 tequila shot glasses so if it was fucked up, not to fuck all the work made with jars, first wanted to see if it was clean.. so i did these guys and looks very weird, i would say contam but its so weird that i dont know.. the trace of water that you make in every hole, it took shape but instead of growing there it like got dried or something, just a few parts mycelium is growing.
Now that i took pictures to upload them i see some green there, but like dry.. i guess the whole LC is contamed :S what do you think?
and maybe it might be on the contam forum, but since it was from LC i thought here are more experts on this to tell me.. if not anyone can move it. thanks! THE PICS THAT ARE UP HERE!!


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Goa, the psychedelic experience______ The power of PSYCHEDELIC MUSIC!!!

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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: luisfer420]
    #15807306 - 02/14/12 08:24 AM (12 years, 4 months ago)

Its hard to say, but from your pics, the jars on the left look contaminated and the ones on the right look as if they have some mycelium growing.

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Offlinekholt17
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15836005 - 02/20/12 02:01 AM (12 years, 4 months ago)

I followed theis tek to a tee. I inoculated 2 lc jars 21 days ago.  I have had a lot of "honey floaters" since day one but i also have some white build up on the top of one jar, and both of my lc jars have a snot like substance floating within.  No dis-colorations other than the "honey floaters" in either jar. Is this normal, and if so, which part is actually the mycelium that i should use? Also, with the jar with the white stuff floating on top i used 4.5cc spore syringe, and in the opposite jar i used 2.5cc. Thank you kindly for any information that can be provided. :smile:

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InvisibleJavadog
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Re: A *beginner friendly* method for making Liquid Culture [Re: kholt17]
    #15836760 - 02/20/12 08:54 AM (12 years, 4 months ago)

Independent of the "floaters", what is the clarity of the solution like?

Is there any buildup on the insider of the jar, near the "water-line"?

I am thinking that you have a bacterial infection.

...but then again, we are talking about and LC, and so this almost goes without saying.

Good luck,

JD


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Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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Offlinekholt17
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Re: A *beginner friendly* method for making Liquid Culture [Re: Javadog]
    #15840691 - 02/20/12 11:55 PM (12 years, 4 months ago)

Yes, in one jar there is a coagulation of white forming on the water line. This tek uses a significant amount of honey, which has turned my l.c.'s yellow. During the day the solutions become clearer, but each  time I swirl they go back to having a yellow hue.  These two jars are being stored in a TOT incubator with an interior temp of 79-80 degrees F.  Is it common to have failed l.c.'s? I ask because of your last comment Javadog.

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InvisibleJavadog
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Re: A *beginner friendly* method for making Liquid Culture [Re: kholt17]
    #15840815 - 02/21/12 12:38 AM (12 years, 4 months ago)

Yes, sugar LCs often suffer from bacterial contamination.  They must
be tested before used in bulk.  I have not gotten a mold from one, but
that is possible too.

The "rime" around the edge of the LC in your one jar indicates bacteria.
As the LC grows, this rime can be formed by mycelium, but it does not
sound like you are at that point yet.

Regarding color, honey will make the LC yellow/goldenish but one thing
that you should expect to see with LCs is the medium becoming clearer
as the LC grows.  Even the color should fade with time.

I am not telling you to chuck anything.  The only test to trust is
a small jar of sterile grains or a BRF cake.

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineOrkidea
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Re: A *beginner friendly* method for making Liquid Culture [Re: Javadog]
    #15869685 - 02/27/12 09:13 AM (12 years, 4 months ago)

Does sugarcanes surgar would work for this?

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Offlinechen
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Re: A *beginner friendly* method for making Liquid Culture [Re: Orkidea]
    #15874132 - 02/28/12 12:26 AM (12 years, 3 months ago)

Would putting micropourous tape over the inoculation hole improve sterility?


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Offlinemindphuck
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Re: A *beginner friendly* method for making Liquid Culture [Re: chen]
    #15874179 - 02/28/12 12:39 AM (12 years, 3 months ago)

-chen

it cant hurt :thumbup:


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OfflineFellowGrower
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Re: A foolproof method for making Liquid culture [Re: mindphuck]
    #15881248 - 02/29/12 01:15 PM (12 years, 3 months ago)

:themoreyouknow:


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Offlinetrippinfloriduh
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15898337 - 03/04/12 09:19 AM (12 years, 3 months ago)

Sounds brilliant!!
Can I use the myc from my BRF cakes once birthed?


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:mushroom2:"Whether drugs lead to illumination or degradation depends on the spirit in which one takes them."

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Offlinezepski
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Re: A *beginner friendly* method for making Liquid Culture [Re: trippinfloriduh]
    #15898753 - 03/04/12 11:35 AM (12 years, 3 months ago)

Quote:

trippinfloriduh said:
Sounds brilliant!!
Can I use the myc from my BRF cakes once birthed?





You wanna make the LC before birth.  Sterilize water inject into pf jar shake as best you can then suck as much water as you can back into the syringe.  I've never done this but i see the dry verm layer causing problems with making the LC chunky but lots of people do it this way.

give these a quick read:

http://www.shroomery.org/forums/showflat.php?Cat=&Board=2&Number=12752577&fpart=1&PHPSESSID=

http://www.shroomery.org/forums/showflat.php/Number/11442976#11442976

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OfflineHyphaeHunter
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Re: A *beginner friendly* method for making Liquid Culture [Re: zepski]
    #15995663 - 03/25/12 07:30 PM (12 years, 3 months ago)

Thanks otto. This tek has worked great so far for the couple LCs that I have made. 
I was curious though if this same procedure would work to sterilize a grain jar?
If I followed all the steps the same and using same kind of jar just substituting my hydrated grains in place of the water.

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Offlinechen
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Re: A *beginner friendly* method for making Liquid Culture [Re: HyphaeHunter]
    #15999322 - 03/26/12 03:10 PM (12 years, 3 months ago)

I've attempted this with 4 jars similar to the ones shown in the tek (salsa jars, same lids) and inoculated two with spore solution (1cc) and the others by scraping spores from a spore print. Both times I was sure to be sterile, flaming needle/scalpel, spraying dettol etc.
It's been two weeks and the jars have yet to have shown any signs of mycelium growth. There is also a sediment forming on the bottom of the jars so I shook them a little.
Shall I start over or is there a chance they'll work? I read that LC only takes a few days to exhibit signs of growth.
:mushroom2:


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OfflineGarrett91
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Re: A foolproof method for making Liquid culture [Re: TheDemon]
    #16500476 - 07/08/12 07:43 PM (11 years, 11 months ago)

Hey just bumping this because I am a beginner and this tek helped me immensely! With in 24 hours i am already seeing major growth! :laugh: thank you so much

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Offlinezepski
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Re: A foolproof method for making Liquid culture [Re: Garrett91]
    #16502265 - 07/09/12 01:50 AM (11 years, 11 months ago)

Quote:

Garrett91 said:
Hey just bumping this because I am a beginner and this tek helped me immensely! With in 24 hours i am already seeing major growth! :laugh: thank you so much





Be careful of quick growth! It usually means contam.  If that jar is after 24hours i would be very very leery.

This was a good one of mine after a few days to a week it was a karo solution of 4%



I dont have any of the LME ones i used after them they were much thicker.  I've seen stuff like the one you had there which the test jars didnt do well.  I'm not saying yours is bad but i would just be sure to test it on a few jars before you do a whole bunch.  I warn cause i've made the mistake wasted 24 jars I wasnt a happy camper.  hehe

After i typed all that I just thought about it but how long before the pic did you shake that or is it on a magnetic stirrer?  I mean it doesnt matter that much but just curious cause I still think it grew way to fast for it to be from spore.

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OfflineGarrett91
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Re: A foolproof method for making Liquid culture [Re: zepski]
    #16502277 - 07/09/12 01:55 AM (11 years, 11 months ago)

I just shook it a little before the photo so the people of the Shroomery could see. I am yet to see any obvious contams but i will be on the lookout!


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InvisibleJavadog
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Re: A foolproof method for making Liquid culture [Re: Garrett91]
    #16502298 - 07/09/12 02:05 AM (11 years, 11 months ago)

I have found that the most common contams of LCs is bacteria.

If your LC turns cloudy over time, then this is the likely culprit.

A clear LC tells you nothing though, so shoot a test jar before you go big.

...and 24 hours is pretty fast, as said above.

Good luck,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

Myco-tek.org

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OfflineGarrett91
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Re: A foolproof method for making Liquid culture [Re: Javadog]
    #16502435 - 07/09/12 03:20 AM (11 years, 11 months ago)

Now that i think about it, it might be bees wax from my honey. Maybe?


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Shop: Mushroom-Hut Liquid Cultures   OlympusMyco.com Olympus Myco Bulk Substrate   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds


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