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InvisibleBarakanaten
Ama-gi
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Registered: 04/14/10
Posts: 6,163
Loc: PNW Mycosphere( Blessed C...
Re: A *beginner friendly* method for making Liquid Culture [Re: cknugget]
    #14914576 - 08/13/11 03:29 AM (12 years, 10 months ago)

Quote:

cknugget said:
I do have one question though. You inject 2cc of spores into the jar for the LC and then when its ready you said to inject 2cc at each injection site. When you say injection site, do you mean 2cc of LC in each (4) holes on my jars? Being 8cc of LC per jar?




a good rule of thumb to remember with spores is...I'll go ahead and quote Doc_t Less is more,

so when you inoculate the LC with spores do so sparingly. 1cc of spore solution is plenty.

when you inoculate your jars with LC you can afford to be a bit more generous with the LC
solution just remember to over do it and throw your moisture content off. 1-2ml/cc of LC
per injection sitex4 should be fine.

here is why less is more in terms of spores,
I could type out my own explenation but it would sound just like an explanation
from AMU's very own boris my buddy so I will just quote from him.

Quote:

Boris said:
A typical single spore germinates into a monokaryotic, or homokaryotic mycelium, which cannot reproduce sexually; when two compatible homokaryotic mycelia join and form a dikaryotic mycelium, that mycelium may form fruiting bodies

Monokaryotic, or homokaryotic mycelium is the cottony/whispy stuff.

Not all monokaryotic mycelia are compatible, they grow out searching for one they can join with sexually. Once they join with another compatible homokaryotic strand, they converge into Dikaryons. Dikaryotic mycelium is the ropey, root like looking growth we call Rhizomorphic strands.

Its important to not shake your jars up right after you inoculate. Just let it drip down the inside of the glass, then don't disturb it till about 30-40% colonization. By not shaking and mixing the spores all around in the jar, your placing plenty of homokaryotic choice all side by side. Thus they don't have to grow out and search for a mate (Filling your jars with monokaryotic mycelium). the choices will be right there side by side, and you will receive a higher amount of Rizomorphic growth in your jars.:thumbup:




hope this helps you out.

:sunny:


--------------------
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Offlinecknugget
Future Trusted Cultivator!
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Registered: 06/09/11
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Re: A *beginner friendly* method for making Liquid Culture [Re: Barakanaten]
    #15020950 - 09/03/11 01:57 AM (12 years, 9 months ago)

Awesome! Thanks for the info!

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OfflinexShroomerx
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Registered: 08/15/11
Posts: 133
Last seen: 8 months, 10 days
Re: A *beginner friendly* method for making Liquid Culture [Re: cknugget]
    #15021292 - 09/03/11 05:35 AM (12 years, 9 months ago)

Very nice and simple, thanks for the tek!

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OfflineShamrocker
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Registered: 02/17/11
Posts: 385
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Re: A *beginner friendly* method for making Liquid Culture [Re: xShroomerx]
    #15022694 - 09/03/11 01:03 PM (12 years, 9 months ago)

Hey OttO. Tried this tek last week as my 1st LC attempt. After the initial boil, I noticed some shit floating around in the water. It was the label adhesive! So I washed out my Braun electric kettle and triple boiled tap water and filled each of three jars with 250 ml, then completed the rest of the steps. Filled 3 syringes with pe, eq and Brazil. Nocced up up 3 brf jars 2 days ago. Will let you know how it went.    Cheers!

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InvisibleMr.PhilCybin
Master Baiter
I'm a teapot

Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15037735 - 09/06/11 12:02 PM (12 years, 9 months ago)

awesome thread. posting so i can find this gem easier when im ready for my LC.

good vibes  :aum:  to all


--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.

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OfflineShamrocker
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Posts: 385
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Re: A *beginner friendly* method for making Liquid Culture [Re: Mr.PhilCybin]
    #15038651 - 09/06/11 03:37 PM (12 years, 9 months ago)

Success! The only jar to fail was the eq, and I used the last dregs of eight month old syringe on that one. Broke open the br and pe jars and both had the sweet smell of mushy goodness! Great tek OttO!

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InvisibleMr.PhilCybin
Master Baiter
I'm a teapot

Registered: 06/13/11
Posts: 11,642
Loc: Gnarnia
Re: A foolproof method for making Liquid culture [Re: Iamthewalrus]
    #15042665 - 09/07/11 12:49 PM (12 years, 9 months ago)

Quote:

Iamthewalrus said:
u definitely don't need a pc...10-15min steam is all liquid needs




so right now i have 5 pf cakes innoced and waiting for colonization, but i think i may have innoced while they were still too warm. i was rushing things...:facepalm: i basically followed RR's pf video to the T other than waiting overnight once they came out of the steamer pot.

now to the relevant part,  i want to make an LC incase my cakes are bad, can i use the same steamer-pot set up like the videos instead of boiling IN the water?
i dont see this as being a problem, but there are far more knowledgeable people on here than me.


--------------------
I'm stupid, Falcon91Wolvrn03 is smart.
I'm ugly, Falcon91Wolvrn03 is beautiful.
I'm a loser, Falcon91Wolvrn03 is a winner.
Someday, I hope to be like Falcon91Wolvrn03 but secretly know I never will.

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Offlineyaknaw
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Registered: 08/19/11
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15045626 - 09/07/11 10:32 PM (12 years, 9 months ago)

nice!

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Offlineyaknaw
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Registered: 08/19/11
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Last seen: 12 years, 4 months
Re: A *beginner friendly* method for making Liquid Culture [Re: yaknaw]
    #15045629 - 09/07/11 10:32 PM (12 years, 9 months ago)

nice!

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InvisibleShroom_Goon
Just Some Guy

Registered: 08/11/11
Posts: 475
Re: A *beginner friendly* method for making Liquid Culture [Re: yaknaw]
    #15056438 - 09/10/11 01:10 AM (12 years, 9 months ago)

:thumbup: Trying this after I go through a few more syringes I have


--------------------
:peace: :heart: :mushroom2:

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Offline2000pm
Wanna be like Myc


Registered: 04/21/10
Posts: 60
Loc: The Sound
Last seen: 12 years, 3 months
Re: A *beginner friendly* method for making Liquid Culture [Re: Shroom_Goon]
    #15127524 - 09/24/11 01:06 PM (12 years, 9 months ago)

Knocked up some ryebags with Mexicana and ATL7 LC made over a year ago with the otto tek.  Been stored and moved and moved again, and sitting in the fridge for the past year.  They are already showing growth after less than a week.           

Great tek!

Edited by 2000pm (09/24/11 01:07 PM)

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Offlinemuze
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Registered: 09/10/11
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Re: A *beginner friendly* method for making Liquid Culture [Re: 2000pm]
    #15166568 - 10/02/11 11:45 AM (12 years, 8 months ago)

this tek looks great! i've got some golden teacher on the way that i am going to use with this method. i calculated that if you use a 3/4 full 1 pint jar you should get roughly 350cc of LC, enough for a LOT of 1/2 pint PF jars or whatever, even using 3-4cc per jar.

my question is, how would i efficiently use this culture with a bulk substrate and what should i use? i'm kind of a noob. i'd like to use a few of those plastic shoeboxes but i'm afraid they won't be tall enough. i'm trying to keep it very cheap and simple. what kind of substrate should i use? the BRF and verm as in PF or something else that i would pasteurize in an oven bag?

love the forums, and the tek!

edit: sorry, to be more clear, i'd like to colonize a bulk substrate right in a tub that would carry the mycelium all the way to fruiting without a lot of maintenance. i'm thinking a larger tub with some holes drilled and filled with silicone or polyfill for gas exchange as i've seen elsewhere on the forums.

Edited by muze (10/02/11 11:48 AM)

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Offlinescatmanrav
Brainy Smurf


Registered: 05/08/04
Posts: 11,483
Loc: Flag
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Re: A *beginner friendly* method for making Liquid Culture [Re: muze]
    #15168013 - 10/02/11 05:03 PM (12 years, 8 months ago)

Use quart jars of wild bird seed. Use the LC ont the jars. When they are colonized, mix them with your bulk substrate in the shoeboxes and go from there.


--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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InvisibleViking14
naturalist
Male

Registered: 09/14/11
Posts: 27
Loc: I'm here. Duh.
Re: A *beginner friendly* method for making Liquid Culture [Re: muze]
    #15168042 - 10/02/11 05:11 PM (12 years, 8 months ago)

Quote:

muze said:
edit: sorry, to be more clear, i'd like to colonize a bulk substrate right in a tub that would carry the mycelium all the way to fruiting without a lot of maintenance. i'm thinking a larger tub with some holes drilled and filled with silicone or polyfill for gas exchange as i've seen elsewhere on the forums.




Sounds like you will need:
For a bulk substrate (Damion's 50/50 Coir Tek)
http://www.shroomery.org/forums/showflat.php/Number/11916595

And something for a monotub. I don't have a link saved for that one but this is the first one I found on search. Search for monotub tek's to find other ideas that may suit you better:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5276282

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Offlinezepski
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Registered: 10/01/11
Posts: 1,292
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Re: A *beginner friendly* method for making Liquid Culture [Re: Viking14]
    #15168605 - 10/02/11 07:14 PM (12 years, 8 months ago)

Can one use karo instead of honey?  If so would the mixture be the same?

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Offlinemuze
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Registered: 09/10/11
Posts: 20
Last seen: 12 years, 6 months
Re: A *beginner friendly* method for making Liquid Culture [Re: Viking14]
    #15170976 - 10/03/11 09:38 AM (12 years, 8 months ago)

Quote:

scatmanrav said:
Use quart jars of wild bird seed. Use the LC ont the jars. When they are colonized, mix them with your bulk substrate in the shoeboxes and go from there.




I know that's the accepted method and that's what everyone is telling me. I'm just interested in experimenting with a syringe I have on the way. Thanks for your help!

Quote:

Viking14 said:
Quote:

muze said:
edit: sorry, to be more clear, i'd like to colonize a bulk substrate right in a tub that would carry the mycelium all the way to fruiting without a lot of maintenance. i'm thinking a larger tub with some holes drilled and filled with silicone or polyfill for gas exchange as i've seen elsewhere on the forums.




Sounds like you will need:
For a bulk substrate (Damion's 50/50 Coir Tek)
http://www.shroomery.org/forums/showflat.php/Number/11916595

And something for a monotub. I don't have a link saved for that one but this is the first one I found on search. Search for monotub tek's to find other ideas that may suit you better:
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5276282




Thanks! I'm definitely gonna use that coir mix. I have a monotub design all picked out, much smaller than the others on the site. Now though im thinking of using less water in the coir mix to allow for a full jar of colonized honey LC mixed in the tub before the casing. From what I read, the biggest potential failure would be contamination because the LC didn't colonize quickly enough. I figure a qt of myc should do it. For reference, I'm using a 1/4 scale mix of damions coir which should fit in my tub and be the correct proportions for roughly a qt of LC (after removing some water from the recipe) or 5 1/2 pint jars such as in the pf tek, whether I'm using wbs or brf in later experiments.

Edited by muze (10/03/11 09:40 AM)

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OfflineKastro
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Male


Registered: 09/08/10
Posts: 193
Loc: Ireland
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Re: A *beginner friendly* method for making Liquid Culture [Re: muze]
    #15172183 - 10/03/11 02:52 PM (12 years, 8 months ago)

sweet, ive got a 500ml jar in the PC now using your Tek!

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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,346
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Re: A *beginner friendly* method for making Liquid Culture [Re: Kastro]
    #15172262 - 10/03/11 03:11 PM (12 years, 8 months ago)

Successfully used this tek to create my first 2 viable LCs this month! B+ and PE uncut!

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Offline_OttO_
Over Stimulated
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15180346 - 10/05/11 08:22 AM (12 years, 8 months ago)

Quote:

josh1 said:
What if you put too little honey in?




Too little is better than too much... It should still grow a little, just not as dense.

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OfflineCircleofFifths
Can I has a mushy?

Registered: 09/21/11
Posts: 336
Loc: Right behind YOU!!!
Last seen: 11 years, 2 months
Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #15180678 - 10/05/11 10:19 AM (12 years, 8 months ago)

Swim has tried this tek for the first time and it seems to be working great. Swim used an empty syringe that had been lying around for about 3 days without the cap (the syringe was used to inoculate 10 jars). For shits and giggles swim sucked up just a little bit of distilled water into the syringe (just to see if there was any microscopic spores left it in it) and squirted it into the water/honey mix. Two days later there is a ton of mycellium growing in swims jar with no signs of contamination. Swim will wait a few more days and see how things progress, if it ends up that it with a contam, no worries, swim was just going to throw the syringe out in the first place. Thanks for the tek.

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