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InvisibleBlutjager
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Re: A foolproof method for making Liquid culture [Re: letsdrinknapalm] * 1
    #5804170 - 06/29/06 06:01 PM (17 years, 6 months ago)

I'm no math whiz so I'm not figuring it out for ya but I use like half a cc per hole x5 holes per jar(I use 5 holes instead of four because it moves faster that way}so That at 2.5 cc's per jar you can imagine that you can do quite a few jars.Just save some of your LC and use it to make more when you start running low.Even if it has used up the nutes and stops growing it will fire right back up again if you throw it into a new solution unless it has been sitting there for quite some time.Good luck


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Offline_OttO_
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Re: A foolproof method for making Liquid culture [Re: Blutjager] * 1
    #5805929 - 06/30/06 03:48 AM (17 years, 6 months ago)

Yeah, good advice Blutjager. You should never be stingy with your LC - just use the remaining bit to make more, or, just take a print or two from your harvest and use that. Even better, take a chunk of tissue from your best looking shroom and use that to start a new LC - then it will be cloned.

Remeber, more LC = faster colonisation.

Inject 10cc and see how you go!


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OfflineMirth
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Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5806206 - 06/30/06 07:52 AM (17 years, 6 months ago)

:thumbup:Thanks for the TEK  :heart:_Otto_ :heart:

Me doing liquid culture - hahaha I feel like a Japanese scientist hehehe.


Maybe when I master this TEK I will alter by adding the holy drink(Coffee) in a small quantity with the honey .


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The ineffable is not always intangible !


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Offline76degrees
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Re: Yep, It's foolproof / noobproof [Re: Mirth] * 1
    #5806440 - 06/30/06 10:17 AM (17 years, 6 months ago)

I'm a total noob and pulled this tek off without a hitch. The jars I inoculated with the LC are catching up to the SS jars which are approximately a week older. If you take into account the 7 day wait, they are nearly the same age - really only seperated by a few days. But I guess that's a few days ahead of contams! SWEET - Thx!


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The world is changed. I feel it in the water. I feel it in the earth. I smell it in the air. Much that once was is lost, for none now live who remember it.


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Offline_OttO_
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Re: Yep, It's foolproof / noobproof [Re: 76degrees] * 1
    #5809042 - 07/01/06 05:54 AM (17 years, 6 months ago)

I'm stoked that it is working so well for everyone!


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InvisibleArrakis
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Re: A foolproof method for making Liquid culture [Re: Iamthewalrus] * 1
    #5809193 - 07/01/06 09:00 AM (17 years, 6 months ago)

Quote:

Iamthewalrus said:
u definitely don't need a pc...10-15min steam is all liquid needs




I'm a big fan of the microwave method (with a 1/2 pint wide-mouthed jar) myself.  This last time, I had forgotten how long I used to microwave it for, and I was too lazy to look it up.  So I microwaved it until it started to boil, and kept it boiling for 30 secs. 

It still worked, even after just 30 seconds of boiling; I've got 6 pint jars almost at full colonization from this LC, at the moment.

From now on, I'm just letting it boil for 60 to 90 secs max.  The method in this thread is a hell of a lot more complicated than karo tek needs to be IMO.  I've been using microwave karo tek (with a few of my personal tweaks) for 2 years now, and have never had a contaminated LC.  For that matter, I've never even had a contaminated jar.

I get the idea that everyone likes to do their LC's a bit differently than everyone else. :grin:


--------------------
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Offline_OttO_
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Re: A foolproof method for making Liquid culture [Re: Arrakis] * 1
    #5812006 - 07/02/06 10:08 AM (17 years, 6 months ago)

Quote:

Arrakis said:
The method in this thread is a hell of a lot more complicated than karo tek needs to be IMO.  I've been using microwave karo tek (with a few of my personal tweaks) for 2 years now, and have never had a conta

I get the idea that everyone likes to do their LC's a bit differently than everyone else. :grin:




Everyones entitled to their opinion.

I never once mentioned using Karo in this 'tek' though.

If you care to read it properly and/or even try it, Im sure you'll realise its not much harder than doing what you describe doing in the microwave - without any water spilling over from tha jar or evaporating, and you are able to use jars with metal lids.


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InvisibleArrakis
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Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5812624 - 07/02/06 02:28 PM (17 years, 6 months ago)

Edited because I was being quite an asshole. :poke:


Edited by Arrakis (07/13/06 03:19 AM)


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Offlineletsdrinknapalm
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Re: A foolproof method for making Liquid culture [Re: Arrakis] * 1
    #5819709 - 07/04/06 12:01 PM (17 years, 6 months ago)

I was making 2 of these yesterday. I used silicone on the injector port just because I had a bottle in the garage. Anyway, I wasn't really expecting a vacuum and it sucked like 5cc out of the syringe before I noticed. Theres about 300ml of water and a tablespoon of honey. Could this become a problem?


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Offlinepfgdude
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Re: A foolproof method for making Liquid culture [Re: blackout] * 1
    #5822393 - 07/05/06 01:57 AM (17 years, 6 months ago)

Could I use micro pore tape insted of the filtering syringe? How exactly do you go about putting silicone over the holes? Just squeeze a big drop there and thats good enough? I read about someone using baby bottles with an inverted nipple full of silicon.


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We swallow with one gulp the lie that flatters us, and drink drop by drop the bitter truth which sets us free.


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Offline_OttO_
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Re: A foolproof method for making Liquid culture [Re: letsdrinknapalm] * 1
    #5822704 - 07/05/06 07:00 AM (17 years, 6 months ago)

Quote:

letsdrinknapalm said:
I was making 2 of these yesterday. I used silicone on the injector port just because I had a bottle in the garage. Anyway, I wasn't really expecting a vacuum and it sucked like 5cc out of the syringe before I noticed. Theres about 300ml of water and a tablespoon of honey. Could this become a problem?




You'll be fine - if anything, it will grow quicker.


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Offlinenoob_queb
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #5830744 - 07/07/06 07:32 AM (17 years, 6 months ago)

Thanks for taking the time to post this TEK. I am brand spanking new to this. My first attempt with syringe innoc into PF cake seems to be stalling so I am having a go with this TEK for my second try.

I inoculated my first honey jar three days ago. I'm seeing some stuff at the bottom of my jar but I think it's the honey sediment you mentioned in an earlier post. I'm hoping it will kick in soon. I guess I expected to see some signs of growth by now seeing how it is supposed to be completely ready in only 7 days. I used a PC to sterilize the liquid.

I will post my progess in a few days. How important is it to keep the jar in a dark place? I have a towel covering my incubator which keeps it completely dark - but somewhere I got the impression that mycelium actually needs a little filtered light.

Anyway, I like the simplicity of this TEK and I hope I'm successful with it. Thanks!


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: noob_queb] * 1
    #5833631 - 07/07/06 10:38 PM (17 years, 6 months ago)

Mycelium doesnt need any light - light is used to initiate pinning, then its important - but while the mycelium is developing - complete darkness is important.


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Offlinebeatnicknick
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Re: A foolproof method for making Liquid culture [Re: Blutjager] * 1
    #5837976 - 07/09/06 06:46 AM (17 years, 6 months ago)

Quote:

Blutjager said:
I'm no math whiz so I'm not figuring it out for ya but I use like half a cc per hole x5 holes per jar(I use 5 holes instead of four because it moves faster that way}so That at 2.5 cc's per jar you can imagine that you can do quite a few jars.Just save some of your LC and use it to make more when you start running low.Even if it has used up the nutes and stops growing it will fire right back up again if you throw it into a new solution unless it has been sitting there for quite some time.Good luck




As I read this post I just thought of something (I'm going to use this Tek soon btw) what if you just added some more honey to the solution instead of making a whole new solution? The only reason it stop growing is because it used up the nutes, so why cant you

A) Take the honey and squirt it in with a crack of the lid open and shake it around with your last remaining mycelium

B) Make some honey water (sterilise it even) and suck it up with your syringe, and squirt it into the hole, leaving even less room for contaminants

Did I just come up with a brilliantly simple way to make this even easier? And skip even more steps?

If this works (and I don't see why it wouldn't) then all you would need to do to continue growing your shrooms is one LC in the fridge, knocked up with honey and water every time the mycelium is low, and then knock up your jars and grow. No spores required.

I'm trying it, and if it works, Otto, make sure you edit your post and add that little detail of what you can do at the end to make the process even simpler for the newbie, and give credit to Nick!


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I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.


Edited by beatnicknick (07/09/06 06:50 AM)


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Invisiblefastfred
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Re: A foolproof method for making Liquid culture [Re: beatnicknick] * 1
    #5838606 - 07/09/06 12:11 PM (17 years, 6 months ago)

> Did I just come up with a brilliantly simple way to make this even easier? And skip even more steps?

No.

> The only reason it stop growing is because it used up the nutes, so why cant you

Because metabolic waste products accumulate. If you were in a spacesuit and somebody kept giving you water and food, you still wouldn't last very long. You might last twice as long, but not much longer. It would also really suck pretty quickly.


-FF


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Invisiblemonstermitch
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Re: A foolproof method for making Liquid culture [Re: fastfred] * 1
    #5838746 - 07/09/06 12:52 PM (17 years, 6 months ago)

Quote:

fastfred said:
If you were in a spacesuit and somebody kept giving you water and food, you still wouldn't last very long.  You might last twice as long, but not much longer.  It would also really suck pretty quickly.

-FF



:rofl2: nice.


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Offlinebeatnicknick
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Re: A foolproof method for making Liquid culture [Re: monstermitch] * 1
    #5839468 - 07/09/06 04:48 PM (17 years, 6 months ago)

Haha god damnit I thought I was onto something... Figured it was too simple to be true.

Whatever. The Tek is still easy as hell.

Edit: If metabolic wastes accumulate in the water, then why not just suck water out with the syringe, then replace with fresh sterilesed water and honey, while still leaving the last remaining mycelium in there?


--------------------
I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.


Edited by beatnicknick (07/09/06 04:51 PM)


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Invisiblefastfred
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Re: A foolproof method for making Liquid culture [Re: beatnicknick] * 1
    #5839528 - 07/09/06 05:05 PM (17 years, 6 months ago)

Sure you could do that. It would be like a continuous flow fermenter or a bioreactor. It's just that it's easier and better to take a small shot of your last LC and use it to inoculate a new one. Either way you'll eventually have to go back to your master culture or spores.


-FF


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Offlinerequieminadream
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Re: A foolproof method for making Liquid culture [Re: fastfred] * 1
    #5840458 - 07/09/06 09:38 PM (17 years, 6 months ago)

Forgive me for being a total newb but let me see if I understand what this is doing. The idea is that after you finish making this Liquid Culture, you can extract a much larger ammount of spores than you put in, and thus it will allow you to create that much more mushrooms?

Like I said I'm still figuring things out and ordering stuff that I need in order to start my first grow.


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It's all part of my secret evil plan.


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Offlinefaceyneck
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Re: A foolproof method for making Liquid culture [Re: requieminadream] * 1
    #5840470 - 07/09/06 09:40 PM (17 years, 6 months ago)

Not, spores; live mycelium.

Mycelium is what spores become when they start colonizing something. If you inject something with liquid culture, you're not injecting spores, which saves lost of time


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