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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
#19261970 - 12/11/13 06:51 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
loki44 said:
How is that I didn't see this thread before? hahaha

well it's seven years old and
Quote:
cronicr said: spores to lc is the worst route to take IMO


This part gives me chills
Quote:
_OttO_ said: Flame sterilise or alcohool swab the tip of the needle then peel back the foil on the jar to expose the small hole you made in the lid. Insert the syringe and inject 2CC of spore solution -

Replace the foil.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 6 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
#19263648 - 12/11/13 11:53 PM (10 years, 1 month ago) |
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Quote:
cronicr said:
Quote:
loki44 said:
How is that I didn't see this thread before? hahaha

well it's seven years old and
Quote:
cronicr said: spores to lc is the worst route to take IMO

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Gabe_18
Ethnobotanist


Registered: 12/29/13
Posts: 120
Loc: Greatest country around.
Last seen: 5 years, 10 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: twistedty]
#19379422 - 01/06/14 08:55 PM (10 years, 23 days ago) |
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Does the size of the jar matter?
-------------------- Mathematics is the language in which God has written the universe- Galileo Galilei I really love plants and fungi!!!!!
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Kazak
Friend


Registered: 12/13/13
Posts: 509
Last seen: 8 years, 5 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Gabe_18]
#19379446 - 01/06/14 09:00 PM (10 years, 23 days ago) |
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Quote:
Gabe_18 said: Does the size of the jar matter?
Nice bump! No it doesn't matter the size. I think wide mouth pint is preferred. But, I am just blowing smoke.
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Nakor420
Fun Guy



Registered: 05/02/13
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Loc: The Spirit World
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Re: A *beginner friendly* method for making Liquid Culture [Re: Kazak]
#19381111 - 01/07/14 06:32 AM (10 years, 23 days ago) |
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Beginner friendly method for lc is to make pf cakes and knock them up with a clean spore syringe....have a nice day.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
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Re: A *beginner friendly* method for making Liquid Culture [Re: Nakor420]
#19382806 - 01/07/14 02:34 PM (10 years, 22 days ago) |
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Quote:
Nakor420 said: Beginner friendly method for lc is to make pf cakes and knock them up with a clean spore syringe....have a nice day. 
clean spore syringe for the most part is an oxymoron
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: twistedty]
#19382861 - 01/07/14 02:42 PM (10 years, 22 days ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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maquina
machine

Registered: 12/30/13
Posts: 10
Loc: Texas
Last seen: 6 years, 5 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#19488038 - 01/28/14 07:12 PM (10 years, 1 day ago) |
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thank you very much, homes
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: maquina]
#19488042 - 01/28/14 07:13 PM (10 years, 1 day ago) |
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Quote:
maquina said: thank you very much, homes
read the subsequent 30 pages to find out why this is old outdated and a bad idea to do. When you're searching these threads limit the age of posts to less than two years to weed out crap methods that died a long time ago.
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hmmm
Strange


Registered: 03/13/13
Posts: 112
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#19497171 - 01/30/14 03:36 PM (9 years, 11 months ago) |
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Thks so much
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dodson

Registered: 10/30/13
Posts: 60
Loc: America
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#19571755 - 02/15/14 08:27 PM (9 years, 11 months ago) |
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How long will an lc last in storage after it is finished colonizing? is it stored at room temperature or chilled and how long does it take to colonize
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_OttO_
Over Stimulated



Registered: 06/01/05
Posts: 2,588
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Re: A *beginner friendly* method for making Liquid Culture [Re: dodson]
#19834387 - 04/12/14 07:21 PM (9 years, 9 months ago) |
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Quote:
dodson said: How long will an lc last in storage after it is finished colonizing? is it stored at room temperature or chilled and how long does it take to colonize
It will last in the fridge for around 6-12 months without a problem.
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_OttO_
Over Stimulated



Registered: 06/01/05
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Loc: Up Over
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta] 1
#19834410 - 04/12/14 07:27 PM (9 years, 9 months ago) |
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Quote:
bodhisatta said:
Quote:
maquina said: thank you very much, homes
read the subsequent 30 pages to find out why this is old outdated and a bad idea to do. When you're searching these threads limit the age of posts to less than two years to weed out crap methods that died a long time ago.
This is not old, outdated or a bad idea.
LC's have always been and will always be another viable and fantastic way to experiment with mycology.
Everyone is jumping on a bandwagon here, and talking about a concept IN THEORY without giving any credit to the fact that, out of hundreds of liquid cultures IN PRACTICE I had almost zero contamination rates.
I'm not the only one -
Quote:
azur said: I'm the biggest fan of liquid cultures. I've had two out of over a hundred contaminate and I was able to see those in the liquid. I find them to be much easier and faster than g2g.
So, just in case anyone is selectively filtering out the purpose of this post I will remind you for the last time -
Quote:
_OttO_ said: Hey guys, I feel like a broken record here, but quit hating on the LC's... Yes, I agree - the best way to start an LC with confidence, is with an agar wedge.
But this wasn't written with that in mind, its just supposed to be a really basic tek in order to appeal to newcomers who may be partially interested/nervous starters to the hobby, without much equipment, in the hope that it sparks some success and interest to encourage people to pursue it further.
I used LC's for many many years, with spores and core biopsies for cloning and had a very very high success rate. I never nocced up huge batches, so never bothered doing test jars and without a lie I honestly had close to 100%, very rarely did I get a contamination.
Thanks - now back to your regularly shceduled shroomery.... 
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DeadHearts

Registered: 07/17/09
Posts: 21,827
Loc: MICHIGAN
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#19834590 - 04/12/14 08:02 PM (9 years, 9 months ago) |
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Quote:
_OttO_ said:
Quote:
bodhisatta said:
Quote:
maquina said: thank you very much, homes
read the subsequent 30 pages to find out why this is old outdated and a bad idea to do. When you're searching these threads limit the age of posts to less than two years to weed out crap methods that died a long time ago.
This is not old, outdated or a bad idea.
LC's have always been and will always be another viable and fantastic way to experiment with mycology.
Everyone is jumping on a bandwagon here, and talking about a concept IN THEORY without giving any credit to the fact that, out of hundreds of liquid cultures IN PRACTICE I had almost zero contamination rates.
I'm not the only one -
Quote:
azur said: I'm the biggest fan of liquid cultures. I've had two out of over a hundred contaminate and I was able to see those in the liquid. I find them to be much easier and faster than g2g.
So, just in case anyone is selectively filtering out the purpose of this post I will remind you for the last time -
Quote:
_OttO_ said: Hey guys, I feel like a broken record here, but quit hating on the LC's... Yes, I agree - the best way to start an LC with confidence, is with an agar wedge.
But this wasn't written with that in mind, its just supposed to be a really basic tek in order to appeal to newcomers who may be partially interested/nervous starters to the hobby, without much equipment, in the hope that it sparks some success and interest to encourage people to pursue it further.
I used LC's for many many years, with spores and core biopsies for cloning and had a very very high success rate. I never nocced up huge batches, so never bothered doing test jars and without a lie I honestly had close to 100%, very rarely did I get a contamination.
Thanks - now back to your regularly shceduled shroomery.... 
Thanks for making that post Otto. I really can't stand all the hate recently regarding LC's. I've also never had any problems with this method and I happen to love it.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts] 1
#19834762 - 04/12/14 08:37 PM (9 years, 9 months ago) |
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There's really no hate. If you're good enough to make a clean LC you're not the person asking questions about it. LC is just fine there's no hate in the private journals among the veteran cultivators but LC is completely useless for the new grower and it will only serve to slow them down and possibly even make them leave this hobby if they don't have success. LC is so damn easy that you could figure it out and know if it would work for you if you know better but most people don't and that's why there is hate because the only people asking are the very people that shouldn't be making one. Sure that's a broad generalization but seriously LC really doesn't have a place with cubensis.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
#19834836 - 04/12/14 08:55 PM (9 years, 9 months ago) |
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I love LC for exotics and right now have 5 on the go. But LC needs to be done under strict sterile conditions and to me things like using ms solution and having open inoculation holes in a lid sound so sloppy. Not using an SAB for that is even worse. I use lids with SHIP and whatman filters, inoculate with clean agar wedges and use 60 cc syringes to cutndown on the number of times the SHIP needs to be pierced.
I have had success with LC. I have also had fail. I learned quickly that the difference between the two was proper sterile tek, and fortunatly I had agar to teach it to me. The problem I have with LC is the notion that they are a shortcut. They are a powerful tool that needs to be treated with respect.
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DeadHearts

Registered: 07/17/09
Posts: 21,827
Loc: MICHIGAN
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Re: A *beginner friendly* method for making Liquid Culture [Re: Pastywhyte]
#19835114 - 04/12/14 09:54 PM (9 years, 9 months ago) |
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What's wrong with asking questions? You guys look like a couple of snobs for no damn reason
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Quexl


Registered: 12/17/13
Posts: 1,443
Loc:
Last seen: 8 years, 10 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
#19835153 - 04/12/14 10:03 PM (9 years, 9 months ago) |
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 there's just better cleaner ways to do it than LC hands down, like g2g and culturing. *LC is more for massively commercial cultivation, isn't it?
Edited by Quexl (04/12/14 10:05 PM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
#19835196 - 04/12/14 10:13 PM (9 years, 9 months ago) |
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I got no problem with people asking questions. If I jumped on someone for simply asking a question I apologize. But I make no apologies for calling out a tek which advocates using spores to inoculate, having holes exposed or opening a lid in open air. These things might have been considered an acceptable practice ten years ago, but we know better today I also dislike the notion that LC is "noob friendly". Agar is noob friendly. If you can get agar down then you are ready for LC.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 7 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: Quexl]
#19835275 - 04/12/14 10:36 PM (9 years, 9 months ago) |
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Quote:
Quexl said:
*LC is more for massively commercial cultivation, isn't it?
no
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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