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Amanitus
Earning my chops


Registered: 03/31/13
Posts: 167
Loc: Buenos Aires
Last seen: 2 years, 3 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cavemandjd]
#18170898 - 04/26/13 09:00 AM (10 years, 9 months ago) |
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Excellent! This is exactly what i needed. Thanks!
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Trancedd
candyflipper



Registered: 09/10/06
Posts: 307
Last seen: 2 years, 3 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#18603431 - 07/24/13 05:46 AM (10 years, 6 months ago) |
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Assuming this works and there appear to be no contams, how long could the mycelium last in the jar for?
I.e, could the ones we made last November still be alive?
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Bilge
longtimenoC


Registered: 08/26/02
Posts: 1,858
Loc: USA
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Re: A *beginner friendly* method for making Liquid Culture [Re: Trancedd]
#18607242 - 07/24/13 10:09 PM (10 years, 6 months ago) |
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I read a post in the thread that mentioned an LC via this method was still fully viable after 8 months in the fridge. Hope this helps.
-------------------- Shopping for your head? Visit HeadShopFinder.com or find Online Head Shops.
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ilovelsd
Stranger
Registered: 05/29/13
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Re: A *beginner friendly* method for making Liquid Culture [Re: Bilge]
#18609881 - 07/25/13 02:12 PM (10 years, 6 months ago) |
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yes thank you for this!
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Greycat16
The cat



Registered: 10/21/10
Posts: 73
Loc: Tip of africa
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#18837515 - 09/13/13 03:58 PM (10 years, 4 months ago) |
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Looks like an awesome tec, must give it a try for sure
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kot_bazilio
Stranger

Registered: 09/26/13
Posts: 1
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Re: A *beginner friendly* method for making Liquid Culture [Re: Greycat16]
#18907219 - 09/29/13 01:47 PM (10 years, 3 months ago) |
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It is really beginner friendly LC tek. Thank you for the detailed description!
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Circle K
What Else Do You Need?



Registered: 05/27/13
Posts: 127
Loc: Israeli Province of Detro...
Last seen: 9 months, 19 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#18907284 - 09/29/13 02:08 PM (10 years, 3 months ago) |
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I followed this tek to the T. It's been a bit over four days, after inoculating with 1CC of spores (All I had left in either syringe), into two sterilized pint jars with 1 1/2 tsp of Karo.
I swirl 'em and I'm not seeing any kind of myc growing, I've got my eyes open for the ropes, but just some tiny, near microscopic bits. I've figured if 2CCs with a half pint takes a week, 1CC in a full pint might take around a month, and that's not factoring in volume.
Could someone point me in the right direction? They're in a cabinet above my fridge, in a nice, warm ~80 degree environment with no altering climate, and the closed, wooden cabinet doors would allow for any needed GE.
 Hillbilly & Tasmanian strains. Two I'm hoping to see a *lot* more of.
-------------------- Hold on tight, to your dream! Unless you have shoeboxes, then hold onto those. Head down to the store, buy some butterfly clips, dub tub your jars, forget about them, move to Kensington, buy a boat you only ever keep in your driveway, become a Pinterest administrator, sell dollar store mugs with rhinestones hot glued all over them.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 12 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
#18907459 - 09/29/13 02:58 PM (10 years, 3 months ago) |
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Quote:
Notahacker420 said: You cannot know 100% if an LC is good or not by visual inspection.
Test jars must be shot up and grown out.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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wyatteb

Registered: 10/02/13
Posts: 85
Last seen: 9 years, 4 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#18985317 - 10/16/13 09:16 AM (10 years, 3 months ago) |
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I followed the tek and at day three here is what my "lc" looks like. I want to note that on day one all that could be seen in the solution is spores. Does anyone know what the hell is floating around in there?
https://files.shroomery.org/files/13-42/193634942-DSC01741.jpg] Thanks
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 12 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
#18985453 - 10/16/13 10:09 AM (10 years, 3 months ago) |
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Nope....maybe spores.....maybe a contam....no way to know for sure.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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wyatteb

Registered: 10/02/13
Posts: 85
Last seen: 9 years, 4 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
#18985537 - 10/16/13 10:38 AM (10 years, 3 months ago) |
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Do the spores expand like that before myc growth? Guess I should wait er out a few more days before I decide to toss. The is my first lc by the way
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 16 days, 12 hours
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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
#18985581 - 10/16/13 10:52 AM (10 years, 3 months ago) |
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Quote:
wyatteb said: Do the spores expand like that before myc growth?
Not really.
Quote:
wyatteb said: Guess I should wait er out a few more days before I decide to toss.
Why would you toss it after only 3 days?
Spores have been known to take 2+ weeks to germinate and show growth.
Then you have to wait for the LC to grow out, then shoot up test jars...
Quote:
wyatteb said: The is my first lc by the way
And I hope it will be your last, for your sake.
Spores are by far the worst way to start an LC.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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wyatteb

Registered: 10/02/13
Posts: 85
Last seen: 9 years, 4 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
#18985630 - 10/16/13 11:05 AM (10 years, 3 months ago) |
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Wow dude if you read the original post then you would see that this teks lc is said to be ready in about a week and has spore injection as one of the options. I followed this tek because it was easy and I had limited supplies. No need to be rude
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
#18985691 - 10/16/13 11:20 AM (10 years, 3 months ago) |
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Quote:
wyatteb said: Wow dude if you read the original post then you would see that this teks lc is said to be ready in about a week and has spore injection as one of the options. I followed this tek because it was easy and I had limited supplies. No need to be rude
I was not rude at all.
I simply stated facts.
I am sorry if you took it personal.
I was sincere about that last part, there are better ways than messing with LCs, especially LCs from spores.
I suggest researching G2G and spawning to bulk if you really want to expand your mycellium.
Not talkin shit about the tek, just being real.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (10/16/13 11:21 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,808
Loc: Canada
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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
#18985707 - 10/16/13 11:24 AM (10 years, 3 months ago) |
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Good god this tek has Fail with a capital F written all over it
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Cb411
New grower



Registered: 08/03/13
Posts: 110
Loc: New York new york
Last seen: 8 years, 10 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: Pastywhyte]
#18985857 - 10/16/13 12:10 PM (10 years, 3 months ago) |
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The thing is this tek is kinda not the greatest. A lot of people have contam problems with lc so if you are going to do it you should do it with the right stuff a pc injections ports. If you don't have that stuff you should just use spore solution to inoculate. Anyway since you started it the pieces floating around are probably caramelised sugar and 3 days isn't enough time to see anything. I know the tek says a week but people are just trying to inform you that that's not there experience . After 2 weeks if its working it should look like this.

After 3 days that jar looked the same as the day I started it. If it does work it dosnt nesecarily mean its not contaminated and should be tested on a pf jar
-------------------- "Reality is a crutch for people who can't handle drugs. "
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cb411] 1
#18985931 - 10/16/13 12:33 PM (10 years, 3 months ago) |
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...and after you injected a needle to your lc to shoot up a test jar, you may have contaminated your lc. That calls for another test jar to be shot up. And then you have it going..
I don't see anything "beginner friendly" about a lc...
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hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: spacechildo]
#18985994 - 10/16/13 12:47 PM (10 years, 3 months ago) |
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I would agree with the statement "there's nothing beginner friendly about LC's". They have a pretty big learning curve and it takes forever for new growers to realize that LC's are best inoculated with known and proven culture on an agar wedge than they are to inoculate with spores.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: A *beginner friendly* method for making Liquid Culture [Re: hamloaf]
#18986043 - 10/16/13 12:56 PM (10 years, 3 months ago) |
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Quote:
hamloaf said: I would agree with the statement "there's nothing beginner friendly about LC's". They have a pretty big learning curve and it takes forever for new growers to realize that LC's are best inoculated with known and proven culture on an agar wedge than they are to inoculate with spores.
QFT
Spores and chunks of mushrooms or pins into a LC is a sure fire way to waist substrates. Noobs try to go LC to skip their agar fears but ironically the best way to start a LC is with agar, but then you could just use the agar wedges and then g2g after that.
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wyatteb

Registered: 10/02/13
Posts: 85
Last seen: 9 years, 4 days
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cb411]
#18986462 - 10/16/13 02:37 PM (10 years, 3 months ago) |
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Thanks for clearing that up bud. I'll post again in a week or so. I'll definitely go for a "better" method once I have the proper items. For now I'm just experimenting bc I had some extra spore solution and no pc
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