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InvisibleDoc_T
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cynosure]
    #14230197 - 04/03/11 08:34 AM (12 years, 9 months ago)

If you aren't seeing anything in two weeks, you can toss it.

... a pack of agar is $1.69, btw. :wink:


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OfflineTrippy_Smurf
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Re: A *beginner friendly* method for making Liquid Culture [Re: Doc_T]
    #14230232 - 04/03/11 08:45 AM (12 years, 9 months ago)

When doing a test cake, how far along does it have to be before you can be sure the source LC is clean?


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Re: A *beginner friendly* method for making Liquid Culture [Re: Trippy_Smurf]
    #14230245 - 04/03/11 08:49 AM (12 years, 9 months ago)

You'll know if it's bad pretty quick.
But you can't call it good for sure until you see fruits.


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Offlinejacobensis
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Re: A *beginner friendly* method for making Liquid Culture [Re: Trippy_Smurf]
    #14230289 - 04/03/11 09:02 AM (12 years, 9 months ago)

awesome tek, Ive been using LC`s for about a year now, never really mentioned it because people freak out and, start screaming all kinds of bull shit. i hope this tek furthers LC`S and gives them the respect they deserve. i use saborouad agar- you should see how fast PAN mycelium grows in that, 3 days and the jar is past done!!


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InvisibleDoc_T
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Re: A *beginner friendly* method for making Liquid Culture [Re: jacobensis]
    #14230304 - 04/03/11 09:08 AM (12 years, 9 months ago)

LCs get all the respect they deserve and then some. IMO.


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OfflineCynosure
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Re: A *beginner friendly* method for making Liquid Culture [Re: Doc_T]
    #14230308 - 04/03/11 09:09 AM (12 years, 9 months ago)

Many thanks! =)

I will definitely try another method the next time around.  I could have had 2 more cakes with the spores I put in those LC's!


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InvisibleDoc_T
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cynosure]
    #14230314 - 04/03/11 09:10 AM (12 years, 9 months ago)

Quick note on spores- spore water is potent, a few drops is all you need in LC or most jars.

Be stingy with spores. :thumbup:


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cynosure]
    #14239076 - 04/04/11 10:22 PM (12 years, 9 months ago)

Quote:

Cynosure said:
I've had two LC's from this tek going for a little over two weeks now and there is no sign of mycelium growth.  There is sediment at the bottom of the jar that I stir every so often, but I'm pretty sure that it isn't mycelium (and it definitely isn't expanding).

Should I go ahead and toss these and give it another shot?




I recently used some 2 year old spores that weren't stored well, and it took 6 weeks for the to germinate in an LC. But they eventually came through, I reckon I was very luck that they did. Looked like only one inoculation point on the bottom of the liquid, may have been lucky enough to get just a few to germinate. It was a fluke I think but gives you an idea of how long it *CAN* take sometimes...


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Invisiblelemonfuzz
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Re: A *beginner friendly* method for making Liquid Culture *DELETED* [Re: Doc_T]
    #14239312 - 04/04/11 11:06 PM (12 years, 9 months ago)

Post deleted by lemonfuzz

Reason for deletion: .



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Invisiblebulkgrownoob

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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #14253938 - 04/07/11 03:44 PM (12 years, 9 months ago)

in the tek, otto says to cover with foil when incubating... would it be ok to use micropore tape to cover that hole and get rid of the foil? or is the hole too big that it would give too much gas exchange this way


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: bulkgrownoob] * 1
    #14335036 - 04/22/11 07:58 PM (12 years, 9 months ago)

Quote:

bulkgrownoob said:
in the tek, otto says to cover with foil when incubating... would it be ok to use micropore tape to cover that hole and get rid of the foil? or is the hole too big that it would give too much gas exchange this way




It would be ok to use micropore tape, you could leave the foil on and put micropore underneath which would work well too - just don't let the micropore get wet.


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InvisibleFischer
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Re: A *beginner friendly* method for making Liquid Culture [Re: lemonfuzz]
    #14337472 - 04/23/11 08:37 AM (12 years, 9 months ago)

Quote:

lemonfuzz said:
Don't mean to hijack...


Quote:

Doc_T said:
If you aren't seeing anything in two weeks, you can toss it.

... a pack of agar is $1.69, btw. :wink:





Awesome! links please?




Just hit up any asian supermarket or the asian food aisle.


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Invisiblesandman420
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Re: A *beginner friendly* method for making Liquid Culture [Re: Fischer]
    #14337488 - 04/23/11 08:52 AM (12 years, 9 months ago)

a little pack of agar agar is $7 everywhere you can find it around here


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Re: A *beginner friendly* method for making Liquid Culture [Re: sandman420]
    #14351722 - 04/25/11 09:08 PM (12 years, 9 months ago)

Here are some pics of my current results using this method.

These were inoculated on 4/11.  The one labeled 'Cambo' was from a print and the 'GT' jar was from MS Syringe.



I have a couple of questions about these LC's...assuming they're viable, how long will they stay viable?  And what can i do to prolong their life?  Should I put them away in the fridge if I don't plan on using them right away?

Also, if you get time, _OttO_, check out my thread I made using your bucketing tek.  I could really use some advice over there.  I feel like im on the right path but could sure use the extra guidance/affirmation from the trusted jedi's around here!   

Countless thanks goes out to _OttO_ for inspiring my recent foray into shroom cultivation!!! :bow2:

:dancingbear:


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OfflineBrownCow
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Re: A *beginner friendly* method for making Liquid Culture [Re: madcapper]
    #14352171 - 04/25/11 10:07 PM (12 years, 9 months ago)

Just want to add that I've got a 2+ yr old lc jar that's been being used constantly, it's still viable currently. It's just 4% karo solution. I think LCs will last in the fridge for 5 yrs honestly.


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Offlinemushr00ms
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Re: A *beginner friendly* method for making Liquid Culture [Re: BrownCow]
    #14352309 - 04/25/11 10:24 PM (12 years, 9 months ago)

Is there a way to make liquid culture without having already harvested mushrooms?


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Offlinemadcapper
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Re: A *beginner friendly* method for making Liquid Culture [Re: mushr00ms]
    #14352371 - 04/25/11 10:33 PM (12 years, 9 months ago)

Quote:

BrownCow said:
Just want to add that I've got a 2+ yr old lc jar that's been being used constantly, it's still viable currently. It's just 4% karo solution. I think LCs will last in the fridge for 5 yrs honestly.



Excellent news!!!  What temp do you store your LC's?


Quote:

mushr00ms said:
Is there a way to make liquid culture without having already harvested mushrooms?



fwiw, I haven't harvested any of these yet (pics above)...the cambos were from a print my friend made...and the Golden Teachers were from syringe procured from an online vendor.


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OfflineMushroomPalace
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #14467257 - 05/17/11 10:31 AM (12 years, 8 months ago)

This is amazing!
I'm trying it at this moment. 30 more minutes and it's finished


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OfflineMushroomPalace
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Re: A *beginner friendly* method for making Liquid Culture [Re: Doc_T]
    #14467307 - 05/17/11 10:42 AM (12 years, 8 months ago)

Quote:

Doc_T said:
If you aren't seeing anything in two weeks, you can toss it.

... a pack of agar is $1.69, btw. :wink:



$1.69? Where do you buy your agar? It's like 10 bucks on Amazon (I live in Belgium, can't find it in the shops where I live).


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InvisibleBase Icks
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Re: A *beginner friendly* method for making Liquid Culture [Re: MushroomPalace]
    #14467315 - 05/17/11 10:45 AM (12 years, 8 months ago)

Try ebay. I'm in the UK and there is a good supplier here. Ebay agar powder.


Edited by Base Icks (05/17/11 10:48 AM)


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