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OfflineTetriana
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Re: A *beginner friendly* method for making Liquid Culture [Re: faceyneck]
    #13297361 - 10/06/10 05:08 AM (13 years, 3 months ago)

I've had mixed results using this tek. Usually 1 out of 2 are bad, so I would make two jars up. :shrug:

I've moved on to using the same formula in baby bottles in my pressure cooker and haven't had a bad lc since.

It's not a very reliable tek, but it did help me with understanding the process of making lc's.


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Offlinefaceyneck
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Re: A *beginner friendly* method for making Liquid Culture [Re: Tetriana]
    #13297372 - 10/06/10 05:15 AM (13 years, 3 months ago)

Quote:

Tetriana said:
I've had mixed results using this tek. Usually 1 out of 2 are bad, so I would make two jars up. :shrug:

I've moved on to using the same formula in baby bottles in my pressure cooker and haven't had a bad lc since.

It's not a very reliable tek, but it did help me with understanding the process of making lc's.





That's a much better system. Also, ideally all cultures should start on agar, so you know they're clean. (...all of mine do, so you know, I do follow my own advice from time-to-time! :smile: )For those who don't mind taking a risk on a LC, this is a pretty good tek... albeit a very old one!

Yeah, the baby bottle method is solid, especially if using a spore syringe acquired from a reliable sponsor. That's as close to a sure thing without agar as there is!

:billymaythumbup:


--------------------
Anything posted here, is total bullshit.
My Meyers-Briggs Personality: INTJ
New growers, or anyone else just needing help; I'm always glad to help right here. :grin:


We give cultivation advice here. :super:


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My Doggy Door Greenhouse!          First Ever Shmuvbox Tek!          Do Manure Right!


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InvisibleShadOWCrack
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Re: A *beginner friendly* method for making Liquid Culture *DELETED* [Re: _OttO_]
    #13307109 - 10/08/10 02:32 AM (13 years, 3 months ago)

Post deleted by longslocksmith

Reason for deletion: .



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Offlinefaceyneck
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Re: A *beginner friendly* method for making Liquid Culture [Re: ShadOWCrack]
    #13307219 - 10/08/10 03:32 AM (13 years, 3 months ago)

Quote:

longslocksmith said:
I just looked at my 1st ks lc it's loaded with myc!!!!
Iam doing one for my ksss and cn
love this tek
thank you!!!!!





I can't stress the importance of testing out LCs on agar.

It's not difficult, expensive, or scary!

Or, if that's still too much, at least do this.

This will save you SOOO much time. Even if your cultures are all nice and clean, it will get you started on a good habit that will rescue you from making jar after jar of funk in the future, without knowing where it came from.

There is no way to know if a culture is clean, unless you place it in 2 dimensions. Even if you get a culture to fruit, it still may have been contaminated the whole time. It's happened to me. :eek:


--------------------
Anything posted here, is total bullshit.
My Meyers-Briggs Personality: INTJ
New growers, or anyone else just needing help; I'm always glad to help right here. :grin:


We give cultivation advice here. :super:


AMU Q & A - We're glad to help
My Doggy Door Greenhouse!          First Ever Shmuvbox Tek!          Do Manure Right!


Edited by faceyneck (10/08/10 07:18 AM)


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InvisibleShadOWCrack
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Re: A *beginner friendly* method for making Liquid Culture [Re: faceyneck]
    #13307412 - 10/08/10 06:06 AM (13 years, 3 months ago)

Cool
the first link you posted was sporeworks b+
the second one was good but I don't have a pc
can you steam them or do you have to have a c
thank you for the feed back. :thumbup:


--------------------
AMU=Amateur Mycologists United

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Offlinefaceyneck
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Re: A *beginner friendly* method for making Liquid Culture [Re: ShadOWCrack]
    #13307541 - 10/08/10 07:14 AM (13 years, 3 months ago)

Quote:

longslocksmith said:
Cool
the first link you posted was sporeworks b+
the second one was good but I don't have a pc
can you steam them or do you have to have a c
thank you for the feed back. :thumbup:





Hahahaha! Wow, that must've been left over from another thread I was responding to. I'll edit it.

...and, I'll post the CORRECT link here!

Hahaha. Here, enjoy a sexy shroom shot as a token of my apology:

Albino Penis Envy, 2nd flush:



...now back to your questions and comments!

Yes, I do believe you could steam sterilize the rice paste. I see no reason why not.

Hey, thanks for bringing that up! Now I can convince ANYONE who grows - even if it's just cakes without a PC! - to prove cultures with plates!

Yeah, go ahead! Try it out!

...come to think of it - I will try it out!

Do me a favor: shoot me a PM in a couple weeks. Make sure I don't forget. Hell, I'll probably make up a few 'rice plates' steam sterilized over this weekend!

Thanks for the inspiration! +5 to you, buddy!

:dancingshroom: :dancingshroom: :dancingshroom: :dancingshroom: :dancingshroom:

EDIT_ you can probably steam sterilize the 'grocery store agar' as well. I'm not 100% confident on that one; maybe 85% confident. I'm over 95% confident on the rice paste, though. :thumbup:

...and I'm glad you appreciate the help. :smile:


--------------------
Anything posted here, is total bullshit.
My Meyers-Briggs Personality: INTJ
New growers, or anyone else just needing help; I'm always glad to help right here. :grin:


We give cultivation advice here. :super:


AMU Q & A - We're glad to help
My Doggy Door Greenhouse!          First Ever Shmuvbox Tek!          Do Manure Right!


Edited by faceyneck (10/08/10 07:20 AM)


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OfflineHarryPotter
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Re: A foolproof method for making Liquid culture [Re: _OttO_]
    #13367108 - 10/21/10 03:41 PM (13 years, 3 months ago)

OLD POST BUT GREAT INFO


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HARRY POTTER


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OfflineChoix Sinaloa
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Registered: 11/02/10
Posts: 992
Loc: California
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #13639605 - 12/15/10 10:16 PM (13 years, 1 month ago)

i purchased the clearest honey, but after i finished the tek i found out its sugar free!

am i doomed!


--------------------
thingy magigy




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InvisibleJavadog
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Re: A *beginner friendly* method for making Liquid Culture [Re: Choix Sinaloa]
    #13639690 - 12/15/10 10:36 PM (13 years, 1 month ago)

I gotta think so....sub-optimal certainly.

What was the replacement sweetener added?

Xylitol is at least a natually occurring substance...

Sorry bro,

JD


--------------------
Boyd Rice told my brother that life is a corny pack of freesakes

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InvisibleBase Icks
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Re: A *beginner friendly* method for making Liquid Culture [Re: Javadog]
    #13640557 - 12/16/10 04:20 AM (13 years, 1 month ago)

Sugar free honey???

:huxleyfacepalm:


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Offlinetriptogod
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Registered: 12/16/10
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #13675387 - 12/23/10 11:52 AM (13 years, 1 month ago)

-OTTO, I Know it's been several years since you posted this, but I wanted to let you know it's still super helpful to us noobs!  Props to you and Thanks!


--------------------


“There are children playing in the streets who could solve some of my top problems in physics, because they have modes of sensory perception that I lost long ago.”
-J. Robert Oppenheimer

Tell a man there are 300 billion stars in the universe and he'll believe you. Tell him a bench has wet paint on it and he'll have to touch to be sure.


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OfflineMr.Anthrax


Registered: 11/22/10
Posts: 207
Last seen: 12 years, 3 months
Re: A *beginner friendly* method for making Liquid Culture [Re: triptogod]
    #13675402 - 12/23/10 11:56 AM (13 years, 1 month ago)

Once it is 100% colonized, how do you transfer it to sayyy, wood chips?


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InvisibleShadOWCrack
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Re: A *beginner friendly* method for making Liquid Culture [Re: Mr.Anthrax]
    #13676046 - 12/23/10 03:00 PM (13 years, 1 month ago)

Get a sterile syringe suck it up lol
Uhhhh knock up some grain then spawn to the chips


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
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:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


Edited by ShadOWCrack (12/23/10 03:06 PM)


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OfflineMr.Anthrax


Registered: 11/22/10
Posts: 207
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Re: A *beginner friendly* method for making Liquid Culture [Re: ShadOWCrack]
    #13676169 - 12/23/10 03:37 PM (13 years, 1 month ago)

I'm surprised this doesn't get contaminants since there is an open hole.


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InvisibleBase Icks
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Re: A *beginner friendly* method for making Liquid Culture [Re: Mr.Anthrax]
    #13676252 - 12/23/10 04:13 PM (13 years, 1 month ago)

Technique > equipment in the right hands.


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OfflineChoix Sinaloa
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Registered: 11/02/10
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Re: A *beginner friendly* method for making Liquid Culture [Re: Base Icks]
    #13676413 - 12/23/10 05:01 PM (13 years, 1 month ago)

using sugar free honey does not work.


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InvisibleShadOWCrack
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Re: A *beginner friendly* method for making Liquid Culture [Re: Choix Sinaloa]
    #13676663 - 12/23/10 06:20 PM (13 years, 1 month ago)

Guess u can add dextrose lol
I like malt but still use this too.
I use this for my fist time and it worked great. I have half a pint of my ks ksss and cn
But have been using just spores, I don't seem to have good luck with my pc and wbs but cake it does fine but fucked cakes lol


--------------------
AMU=Amateur Mycologists United

AMU ------->:opensign:--->:goatse:<---:opensign:<------Q&A
:squirrelnut:I'm just a squirrel man, trying to get a nut:urinate:

:v::v:

:opensign: VVV Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV :opensign:


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OfflineArJunaSpore
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #13706331 - 12/30/10 02:35 PM (13 years, 1 month ago)

This TEK works great. I used it to make my first batches of LC. I had tried going straight from 2 different spore syringes to agar petri dishes and had no growth of any kind in two weeks.

So I made up three 1/2 pint jars of this tek and inoculated two of them from 2 different spore syringes and one culture syringe of Lentinula edodes (Shiitake). One week later all three look just like the image of the completed tek in the first post of this thread. I am about to transfer them to rye grain subs I made a few days ago.

I used plain old honey from Costco. I just bought some raw organic honey for the next go at this.

One of the jars has black specks in it because the spores came from an EarthsTongue.com "New Moon" type spore syringe, which is in a black liquid preservative of some kind. I can only hope that the black specks are entirely from the syringe and not from anything else. It appears so to me.

Thanks for the TEK. It's so simple and effective.


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Invisiblevirus1824
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #13706684 - 12/30/10 03:27 PM (13 years, 1 month ago)

Quote:

d incubate this in a dark place at 29C or 82F




I'm hearing 70-75F for incubation which is it?


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Offline_OttO_
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Re: A *beginner friendly* method for making Liquid Culture [Re: virus1824]
    #13710473 - 12/31/10 08:14 AM (13 years, 1 month ago)

Anywhere from 70-80 is fine. The closer to 80 the faster the colonisation times...

Still to this day I've lost only one LC to this method, so happy it still helps people and -

HAPPPY HEW YEAAAR


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