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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10576367 - 06/26/09 11:51 AM (14 years, 7 months ago)

My temps are by the books not recomended. I live in Portugal and the temps got to 90 in some days. The temps are very important when you inoc though as the heat can kill the spores.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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Offlinedieselkush
Stranger


Registered: 02/02/09
Posts: 582
Last seen: 14 years, 4 months
Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10576502 - 06/26/09 12:20 PM (14 years, 7 months ago)

yea, I know theres a heat wave in the us. my temps are usually optimal. bit lately theyve ben rising into the high 80s close to 90. the mycelium seems do be doing ok. I inoculated some jars that where still pretty warm to the touch on a hot day and there colonizing fast. im not sure what temp spore death occurs..


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Invisible13shroomsM
Lightning Shaman
I'm a teapot User Gallery


Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
Trusted Cultivator
Re: A *beginner friendly* method for making Liquid Culture [Re: dieselkush]
    #10576623 - 06/26/09 12:49 PM (14 years, 7 months ago)

Quote:

im not sure what temp spore death occurs




if Im not mistaken it 104F,

Im dealing with heat issues also, they will still grow but tend to slow due to high temps and your flushes wont be as good as they would have been in optimal conditions.  my temps are in the 80-90F range and everything still colonizes just needs a couple more days to complete.  oh and mold likes to onset alot quicker in the high temps only giving a flush or 2 instead of 4 or 5.

I have to get a window A/C unit to cool my room down to 75F.

just some fyi.

peace 13:mushroomgrow:


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:takingnotes:A M U:helpdesk:
Click here ^ for the AMU forum
VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV
"Man is the sex organ of the machine world" ~  Marshall McLuhan


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Offlinepickboy87
Shroom n00b
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
    #10577508 - 06/26/09 03:51 PM (14 years, 7 months ago)

Wow, I've been digging around on the forums and had been debating doing a LC for a while, but could only find rather complex and time consuming teks (not that I don't have time for them). Came in today to check the forums for more useful information and found this.

I'm on my first (well technically second) attempt at growing mushrooms, but this time I'm much more serious about it and would love to get into a small bulk grow. I just want to say a large THANKS! to _OTTO_. I can't wait to give it a try (just waiting on spores). When I can give out 5 shrooms, you'll be one of the first people to get it :laugh:


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My first harvest:



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OfflineThunderShroomer
Sensei
I'm a teapot User Gallery


Registered: 05/16/09
Posts: 44
Last seen: 5 years, 2 months
Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10581608 - 06/27/09 01:22 PM (14 years, 7 months ago)

Quote:

ThunderShroomer said:





So what does everyone think? Is this good growth or not... Lots of tiny spider web-like filaments, Some very small, some a little more than a centimeter long.


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


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Offlinepickboy87
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Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10581957 - 06/27/09 03:13 PM (14 years, 7 months ago)

You got a better pic than that?


--------------------
My first harvest:



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Offlinedieselkush
Stranger


Registered: 02/02/09
Posts: 582
Last seen: 14 years, 4 months
Re: A *beginner friendly* method for making Liquid Culture [Re: pickboy87]
    #10581978 - 06/27/09 03:25 PM (14 years, 7 months ago)

lol small pic. I was curious would gypsom speed up a lc if used? I know its sped up the colonization of my jars significantly.


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OfflineThunderShroomer
Sensei
I'm a teapot User Gallery


Registered: 05/16/09
Posts: 44
Last seen: 5 years, 2 months
Re: A *beginner friendly* method for making Liquid Culture [Re: pickboy87]
    #10585878 - 06/28/09 11:33 AM (14 years, 6 months ago)

Here are some better ones.... But in order to not get this thread off topic, I have moved the conversation here



--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


Edited by ThunderShroomer (06/28/09 11:46 AM)


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Invisibleexplosiveoxygen
Prophet of TGMM


Registered: 07/10/09
Posts: 1,255
Re: A foolproof method for making Liquid culture [Re: Apache]
    #10705588 - 07/19/09 06:55 PM (14 years, 6 months ago)

Can the pot part be replaced with a Pressure Cooker?

Is the honey and the heat what make the spores germinate in this jar as opposed to inside a spore syringe?

If I wanted to take mycelium from the middle of a mushroom and throw it in here how would I go about it?


--------------------
The Great Mycelium (TGMM) is more than you and me, we are all part of One.


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Offlinesmurfyshmu
It gets crazy
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Registered: 08/10/06
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Re: A foolproof method for making Liquid culture [Re: explosiveoxygen]
    #10705614 - 07/19/09 07:00 PM (14 years, 6 months ago)

I hate to be one of "those people" but the search function is useful

like i found here
and the water is what makes the spores germinate. the honey provides a nutritive substance to live off of. Heat will kill your spores..


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Invisibleexplosiveoxygen
Prophet of TGMM


Registered: 07/10/09
Posts: 1,255
Re: A foolproof method for making Liquid culture [Re: smurfyshmu]
    #10705817 - 07/19/09 07:41 PM (14 years, 6 months ago)

Quote:

smurfyshmu said:
I hate to be one of "those people" but the search function is useful

like i found here
and the water is what makes the spores germinate. the honey provides a nutritive substance to live off of. Heat will kill your spores..



If the water is what makes your spores germinate then how do spore syringes last weeks and months?


--------------------
The Great Mycelium (TGMM) is more than you and me, we are all part of One.


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Offlinesmurfyshmu
It gets crazy
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Re: A foolproof method for making Liquid culture [Re: explosiveoxygen]
    #10708108 - 07/20/09 07:16 AM (14 years, 6 months ago)

Sometimes you will find mycelium strings in a syringe. I believe its the added nutrients that help it along though.


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OfflineMescalitoTed
Stranger
Registered: 06/25/09
Posts: 11
Last seen: 14 years, 5 months
Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10717288 - 07/21/09 07:48 PM (14 years, 6 months ago)

But how do you know that the mycellium growing in the LC is from the spores you've added and not the endospores that reside in Honey? Is there a way to differentiate the two?


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InvisibleDoc_T
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Posts: 42,395
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Re: A *beginner friendly* method for making Liquid Culture [Re: MescalitoTed]
    #10717322 - 07/21/09 07:52 PM (14 years, 6 months ago)

You shoot a test jar. It's the only way.


--------------------
You make it all possible. Doesn't it feel good?


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OfflineMescalitoTed
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Registered: 06/25/09
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Re: A *beginner friendly* method for making Liquid Culture [Re: Doc_T]
    #10717561 - 07/21/09 08:34 PM (14 years, 6 months ago)

And how long do you have to wait to see if its contaminated? Will contamination show immediately?


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OfflineArcanePerception
The only rule isthe golden rule
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Registered: 04/30/05
Posts: 435
Last seen: 13 years, 11 months
Re: A *beginner friendly* method for making Liquid Culture [Re: MescalitoTed]
    #10717760 - 07/21/09 09:07 PM (14 years, 6 months ago)

Wow cant believe this thread was bumped again.


--------------------
Oh, welcome to this world of fools
Of pink champagne and swimming pools


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Offlinequadracer
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Registered: 12/14/08
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Re: A *beginner friendly* method for making Liquid Culture [Re: ArcanePerception]
    #10717879 - 07/21/09 09:30 PM (14 years, 6 months ago)

I've heard that honey contains anti-fungal properties.  Has anyone come across this?


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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: A *beginner friendly* method for making Liquid Culture [Re: quadracer]
    #10717911 - 07/21/09 09:39 PM (14 years, 6 months ago)

Honey is a preservative. If used in big amounts, spores won't develop.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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Offlinedm_0001
Stranger
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Registered: 06/03/09
Posts: 32
Last seen: 13 years, 7 months
Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10757361 - 07/28/09 06:38 PM (14 years, 6 months ago)

... just a clarification.

After I see growth in the jar (i.e. one week later) do I need to fill up all my syringes with the LC? Or can I just leave the jar in my fridge and retrieve the LC with a syringe only when I need it.

Thanks ahead of time.


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InvisibleAlexP
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Registered: 06/04/09
Posts: 2,270
Loc: Europe
Re: A *beginner friendly* method for making Liquid Culture [Re: dm_0001]
    #10757381 - 07/28/09 06:42 PM (14 years, 6 months ago)

You may leave it in the fridge. :smile:


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


Extras: Filter Print Post Top
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Shop: Original Sensible Seeds Bulk Cannabis Seeds   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   North Spore Injection Grain Bag   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract


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