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OfflineCmmaples
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Re: A *beginner friendly* method for making Liquid Culture [Re: fastfred]
    #10235476 - 04/26/09 09:07 AM (14 years, 9 months ago)

Just a quick question.. Can you use a resealable injection cap on these? I am curious if there needs to be gas exchange or any fresh air...


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Offlinekagera
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Re: A *beginner friendly* method for making Liquid Culture [Re: Cmmaples]
    #10235518 - 04/26/09 09:24 AM (14 years, 9 months ago)

for anything that is going to grow, It needs gas exchange. IF your going to put a resealable injection cap, you should make another hole and stuff poly fill in it.


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OfflineAlienTechKilla
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Re: A *beginner friendly* method for making Liquid Culture [Re: kagera]
    #10240697 - 04/27/09 01:20 AM (14 years, 9 months ago)

Knocked up 3 jars today using this tek. Jars are a yellowish brown after agitation and I placed a second piece of foil on the lid to cut down on contams hopefully. Incubating now at around 78 degrees. I'll definitely post my results.


ATK


Edited by AlienTechKilla (04/27/09 01:23 AM)


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InvisibleGerman Kahuna
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlienTechKilla]
    #10240722 - 04/27/09 01:31 AM (14 years, 9 months ago)

Quote:

I placed a second piece of foil on the lid



Wrong. You want none instead of two. If you seal the jars with foil, how's there going to be any gas exchange? Use either micropore tape over the hole or stuff polyfill into it. If you want an additional barrier you can use a coffee filter held in place by a rubber band.


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".


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OfflineAlienTechKilla
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Re: A *beginner friendly* method for making Liquid Culture [Re: German Kahuna]
    #10240794 - 04/27/09 01:52 AM (14 years, 9 months ago)

I could be wrong and I'm not trying to contradict you, but putting foil over the top of something doesn't make it air tight. If so, how did my PF Tek cakes get gas exchange. Same seal. I deffinitely  replace the top layer of foil  with some poly per your suggestion. The  hole is so small it probably doesn't NEED a filter at all. I just have had issues with to much fresh air durring colonization. Like with my mono. Thanks for the sugestions.


ATK


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Invisiblefastfred
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlienTechKilla]
    #10243610 - 04/27/09 03:45 PM (14 years, 8 months ago)

Ok, you guys are off track.

The question was about storage in the fridge.  You don't need or want any gas exchange there.  It's also fine to use a sealed plug or whatever.  But it is a waste of time and money.  And you might get problems with pressure buildup, which can cause air to get sucked into your jar contaminating it.

Always use a foil cap over everything you do.  It's standard practice and it's there to prevent contams from falling into your culture.  It's not airtight.


As far as gas exchange in any LC...  It's not important.  Your culture depends in the dissolved oxygen (DO) in the liquid.  It doesn't really matter how much air exchange you have, it won't change the DO level in the liquid.  Stirring, swirling, or aeration/aspiration will increase the DO and allow you to grow up a much thicker LC.  But it's fine to just let it go with what's in there and then referigerate it.  You don't need it to be very thick, and it is a lot harder to suck it out when it's too thick.

The easiest way to do these LCs is simply drill a hole in a jar lid, fill it, and put foil over the top.  Then sterilize.  You don't need plugs or filters or any of that happy horseshit.  The foil keeps contams from falling in and keeps the whole top clean.

The only time you need plugs, filters, or the like is if you have some sort of situation where you can't keep your jar upright or it has to be placed in a really dirty environment.


-FF


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Offlineyougotgot
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #10348191 - 05/16/09 11:32 AM (14 years, 8 months ago)

I'm going to try this one lots of pics and how too thank you for making this lc how to


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InvisibleEid

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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #10367767 - 05/20/09 01:30 AM (14 years, 8 months ago)

:cookiemonster:


Edited by Eid (05/17/11 11:20 AM)


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OfflineScucci
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Re: A *beginner friendly* method for making Liquid Culture [Re: Eid]
    #10382089 - 05/22/09 01:30 PM (14 years, 8 months ago)

Useful thread bump. (if it's not stickied... I come here from bookmarks, so I have no idea).

Anywho, just to add... some people are talking about how honey is expensive and how Karo is cheaper, but how honey works better and they wish they could use it.

Well, next time you're out and about, and you drive by a KFC or Bonjangles, stop in... get a glass of tea or something, and look around or ask the person behind the counter if you can get a few dozen honey packets. Most of the time, they'll just hand them to you or they'll be sitting out for the taking. Free honey, and it works just fine for starting LC.

Basically any place that serves biscuits will also have honey packets or dipping tubs. Just check around, free is always better because, hey... free, duh.


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InvisibleAlexP
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Re: A *beginner friendly* method for making Liquid Culture [Re: Scucci]
    #10509864 - 06/15/09 09:56 AM (14 years, 7 months ago)

Great!


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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OfflineFungiFarmer
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Re: A *beginner friendly* method for making Liquid Culture [Re: ruslah]
    #10510423 - 06/15/09 12:14 PM (14 years, 7 months ago)

Quote:

ruslah said:
I think that I will try 3 different jars all up. One with flesh from my first fruits that have popped up in the past week







Is it possible to make an LC with flesh from a fresh mushroom?


--------------------
:mushroom2::mushroom2::mushroom2:


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InvisibleAlexP
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Re: A *beginner friendly* method for making Liquid Culture [Re: FungiFarmer]
    #10522806 - 06/17/09 09:58 AM (14 years, 7 months ago)

Hummm I was doing this LC last night... and I was smoking some really good grass. I sterilized everything up and I use alcohol to clean my hands while I'm working and to clean the things I touch. When I grabbed my LC bottle I noticed it was still warm so I covered it with a napkin, soaked in alcohol, in my glove box. I had a candle going for sterilizing the needle and all of a sudden it all bursts in flames! All my lab is burning like hell! HAHAHAHAH

The good thing is I've received training to put fires out so it was all over in seconds... I know it sounds obvious but never use a flame near alcohol... it doesn't need contact. The vapors are a lot more inflammable.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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Offlinedetuned radio
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10522903 - 06/17/09 10:22 AM (14 years, 7 months ago)

awesome tutorial!

thank you!


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OfflineThunderShroomer
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Re: A *beginner friendly* method for making Liquid Culture [Re: detuned radio]
    #10553577 - 06/22/09 01:17 PM (14 years, 7 months ago)

So I tried this method with a good spore print that i had taken myself on a piece of paper, then folded up and put in a ziplock bag. It has been two weeks and both the jars I tried have no growth whatsoever. No contamination, no myc, nothing but honey slightly clumping together. But this can be put back into solution with a quick twirl of the glass. It has been at about 74 degrees in the dark for this time. Any advice?


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


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OfflineThunderShroomer
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Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10567560 - 06/24/09 10:08 PM (14 years, 7 months ago)

Correction. I do, in fact, have growth... I think:


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


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InvisibleAlexP
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Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10567830 - 06/24/09 10:57 PM (14 years, 7 months ago)

Quote:

Correction. I do, in fact, have growth... I think:




That's your growth?... =/


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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OfflineThunderShroomer
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10573406 - 06/25/09 08:34 PM (14 years, 7 months ago)

Quote:

AlexP said:
Quote:

Correction. I do, in fact, have growth... I think:




That's your growth?... =/



Yea... I think so... after it is shaken lightly to break up the clumps. But there are clearly tiny spiderweb-like filaments. It is pan subs. Good or bad?


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


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InvisibleAlexP
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Re: A *beginner friendly* method for making Liquid Culture [Re: ThunderShroomer]
    #10573643 - 06/25/09 09:05 PM (14 years, 7 months ago)

I don't see any growth... maybe there is...? My LC was ready in one week. A big disform white mass at the bottom. It was Pan. Cyans.


--------------------
"To feel today what one felt yesterday isn't to feel - it's to remember today what was felt yesterday, to be today's living corpse of what yesterday was lived and lost." Fernando Pessoa

"When one creates phantoms for oneself, one puts vampires into the world, and one must nourish these children of a voluntary nightmare with one's blood, one's life, one's intelligence, and one's reason, without ever satisfying them." Eliphas Levi

"The joy of life consists in the exercise of one's energies, continual growth, constant change, the enjoyment of every new experience. To stop means simply to die. The eternal mistake of mankind is to set up an attainable ideal." Aleister Crowley

"The Universe is an artistic catharsis." Artifex Infinitum


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OfflineG1assjaw
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10573653 - 06/25/09 09:06 PM (14 years, 7 months ago)

Yeah this tek works great! Though I found using less honey works better.


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OfflineThunderShroomer
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Re: A *beginner friendly* method for making Liquid Culture [Re: AlexP]
    #10576355 - 06/26/09 11:49 AM (14 years, 7 months ago)

Quote:

AlexP said:
I don't see any growth... maybe there is...? My LC was ready in one week. A big disform white mass at the bottom. It was Pan. Cyans.




What temp did you keep it at etc.? I will be trying Pan Cyans very soon and any tips would be helpful.


--------------------
"Happiness is when what you think, what you say, and what you do are in harmony." -Me announcing that I'm about to take a massive shit.



:peace: on :earth:


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