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afroman25
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Registered: 01/28/09 
Posts: 23
Last seen: 14 years, 10 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#9876461 - 02/27/09 11:40 AM (14 years, 10 months ago) |
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Nice tek OTTO I'm gonna try it this weekend
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n3kkidninj4
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Registered: 03/06/09
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Re: A *beginner friendly* method for making Liquid Culture [Re: afroman25]
#9926326 - 03/07/09 12:03 AM (14 years, 10 months ago) |
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I am just days away from starting my journey with this hobby. I read all thru this and see that it says I dont need a pc. Do I not need to sterilize my substrate before injecting the lc. Im planning on doing BRF cakes.
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OttoGenerated
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Registered: 02/23/09
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Re: A *beginner friendly* method for making Liquid Culture [Re: n3kkidninj4]
#9926573 - 03/07/09 12:58 AM (14 years, 10 months ago) |
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Awesome thread, very helpful. I'm more interested in doing it "right" than doing it easy, but having it written out for a beginner this way makes it so much easier to understand the more advanced techniques.
Thanks, Otto.
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: A *beginner friendly* method for making Liquid Culture [Re: n3kkidninj4]
#9926600 - 03/07/09 01:04 AM (14 years, 10 months ago) |
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Quote:
n3kkidninj4 said: I am just days away from starting my journey with this hobby. I read all thru this and see that it says I dont need a pc. Do I not need to sterilize my substrate before injecting the lc. Im planning on doing BRF cakes.
yes you do, but you can just steam/boil them in a large covered pot (no PC) for 90min.
--------------------
A M U Click here ^ for the AMU forum VVV AMUs Free Active/Edible/Exotic Spore Print or Syringe or Edible Culture Trade Thread VVV "Man is the sex organ of the machine world" ~ Marshall McLuhan
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n3kkidninj4
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Registered: 03/06/09
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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
#9926692 - 03/07/09 01:29 AM (14 years, 10 months ago) |
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but would it be quicker/easier just to use the pc the sterlize the brf cakes? that only takes 60 mins yes?
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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
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Re: A *beginner friendly* method for making Liquid Culture [Re: n3kkidninj4]
#9926739 - 03/07/09 01:46 AM (14 years, 10 months ago) |
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its best to sterlize the jars. to cut down on contams
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: A *beginner friendly* method for making Liquid Culture [Re: anakin3197]
#9928312 - 03/07/09 01:14 PM (14 years, 10 months ago) |
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Quote:
but would it be quicker/easier just to use the pc the sterilize the brf cakes? that only takes 60 mins yes?
Of course! If you're the least bit serious about this hobby you'll get and use a PC.
It's faster (30 min is plenty for 1/2 pints), it's more reliable (less contams), and it consumes less energy and water.
If you're boiling 90 min vs PCing for 30 min you'll save the cost of a cheap PC in a dozen runs or so just in power costs.
-FF
-------------------- It drinks the alcohol and abstains from the weed or else it gets the hose again. -Chemy The difference between the substances doesn't matter. This is a war on consciousness, on our right to the very essence of what we are. With no control over that, we have no need to speak of freedom or a free society. -fireseed "If we are going to have a war on marijuana, the least we can do is pull the sick and the dying off the battlefield." -Neal Levine (MPP) I find the whole "my drug should be legal but yours should be illegal" mindset disgusting and hypocritical. It's what George Bush and company do when they drink a cocktail and debate the best way to imprison marijuana users. -Diploid
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jdolphinbabe
One Who Seeks 2 Know



Registered: 09/26/08
Posts: 402
Loc: Washington
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#9928854 - 03/07/09 02:46 PM (14 years, 10 months ago) |
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wow i have totally wanting to try a LC but some what intimidated well NO MORE! this is simple and easy... thanks so much this thread ROCKS!
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j0nnyb0y05
IBelieveInBelivin



Registered: 09/01/07
Posts: 2,076
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Re: A *beginner friendly* method for making Liquid Culture [Re: jdolphinbabe]
#9930178 - 03/07/09 07:26 PM (14 years, 10 months ago) |
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well I just used this tek last weekend and it is going supurb. I just used a ker jar instead, a little less honey that suggested, and shot it up with 1.5 cc's of amazon. Its been 5 days and it is just flowing with myc. i believe it will be fully colonized by monday. I then plan to inoc some cakes. wish me luck and thanks for the easy tek otto.
-------------------- .....Healing Of People Everywhere.....
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: A *beginner friendly* method for making Liquid Culture [Re: fastfred]
#9932824 - 03/08/09 10:29 AM (14 years, 10 months ago) |
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Quote:
fastfred said:
Quote:
but would it be quicker/easier just to use the pc the sterilize the brf cakes? that only takes 60 mins yes?
Of course! If you're the least bit serious about this hobby you'll get and use a PC. -FF
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wigio
Courier of Time



Registered: 08/06/08
Posts: 22
Last seen: 14 years, 5 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Blutjager]
#9933240 - 03/08/09 12:32 PM (14 years, 10 months ago) |
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Easy to follow, and the pictures are mint. Will do tonight. Thanks!
-------------------- Live and forgive.
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DarkLotus
Its All Sunshine...



Registered: 02/17/09
Posts: 135
Loc: Right next to the Beach, ...
Last seen: 12 years, 4 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#9944564 - 03/10/09 08:53 AM (14 years, 10 months ago) |
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Just made my first LC today using this tek. Thank you Otto for breaking it down laymen. Before this tek I thought it was going to be awhile before I started making LC's. will post my results...
-------------------- "keep your schnoz clean ears open choppers zipped up food clothes medicine 'cuz hungry naked sick sucks" -Aesop Rock "Walk through the time flux hand and hand with clear mind Thoughts are harmonious like the rhythm of wind chimes" -Twiztid
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shroomiin
unprofound


Registered: 12/24/07
Posts: 2,470
Loc: Zone 6
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#10038429 - 03/25/09 11:53 AM (14 years, 10 months ago) |
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nice
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Jezuz2point0
Stranger
Registered: 03/19/09
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Last seen: 14 years, 7 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#10049221 - 03/26/09 09:12 PM (14 years, 10 months ago) |
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I was just wondering if I could use the fluffy mycelium of a freshly birthed cake??? Would either stage matter as long as it's fresh, say second or third flush??
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lethargic
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Registered: 03/09/09
Posts: 351
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Last seen: 12 years, 7 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: Jezuz2point0]
#10049238 - 03/26/09 09:16 PM (14 years, 10 months ago) |
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cool tek, i am sure this well help many!
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Edited by lethargic (03/28/09 06:47 PM)
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matan
newcommer


Registered: 02/25/09
Posts: 27
Last seen: 10 years, 8 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#10054405 - 03/27/09 03:17 PM (14 years, 9 months ago) |
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Just Thank you man...
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pftek
1337


Registered: 08/18/08
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Last seen: 13 years, 3 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: matan]
#10054875 - 03/27/09 04:20 PM (14 years, 9 months ago) |
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1) In the original tek posted you stated you do 1 tablespoon for every 250 ml. That's about 6%. Is that on the high end? If you had to give a range that'd be very acceptable - would you say 2-8%? so for 250 ml between 5 ml of honey to 20 ml of honey? Cause I hear a lot of people going towards 3%.
2) Also is it possible to this method but in a larger quantity and just multiply?
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scoops
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Registered: 03/10/09
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Last seen: 13 years, 1 month
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Re: A *beginner friendly* method for making Liquid Culture [Re: pftek]
#10055156 - 03/27/09 04:58 PM (14 years, 9 months ago) |
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[[1) In the original tek posted you stated you do 1 tablespoon for every 250 ml. That's about 6%. Is that on the high end? If you had to give a range that'd be very acceptable - would you say 2-8%? so for 250 ml between 5 ml of honey to 20 ml of honey? Cause I hear a lot of people going towards 3%. ]]
6% will work, but I would try to stay closer to 4%. I do 1 tablespoon per 350 ml. Im pretty sure you can go up to about 10% before the sugar levels are toxic for the mycelium.
[[2) Also is it possible to this method but in a larger quantity and just multiply?]]
yes! it sure is.
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ruslah
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Registered: 02/18/09
Posts: 40
Loc: Melbourne, Australia
Last seen: 7 years, 9 months
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
#10146888 - 04/11/09 09:39 AM (14 years, 9 months ago) |
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As everyone has said already, thanks so much for such an easy tutorial. Very keen to give it a try once I get back from holidays.
I think that I will try 3 different jars all up. One with flesh from my first fruits that have popped up in the past week (Cambodian), one with the Cambodian spores that are still in the syringe and another with the Hawaiian syringe which I haven't used yet.
Reason that I'm using the flesh for a jar as well as one with spores of the same strain is because the syringe is of the 'New Moon' variety that I purchased from ET (rookie mistake). For those that don't know, the solution in these syringes are black/purpleish colour. It would be hard to tell what's going on in my LC if there's all this black crap floating around too. I also didn't keep this syringe refrigerated after I first used it either, so don't know if it's any good at all.
Quick question - is there any need at all to give the LC a swirl every now and then after it's been sitting in the fridge for a few weeks after the myc has fully grown?
-------------------- Boats 'n hoes.
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fastfred
Old Hand



Registered: 05/17/04
Posts: 6,899
Loc: Dark side of the moon
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Re: A *beginner friendly* method for making Liquid Culture [Re: ruslah]
#10148313 - 04/11/09 03:13 PM (14 years, 9 months ago) |
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> is there any need at all to give the LC a swirl every now and then after it's been sitting in the fridge for a few weeks
No. You want to keep temp and DO levels down to limit metabolism during storage.
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