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Offlineacidbuba
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Registered: 01/31/06
Posts: 125
Last seen: 16 years, 6 months
Re: A foolproof method for making Liquid culture [Re: _OttO_] * 1
    #5799141 - 06/28/06 12:04 PM (17 years, 7 months ago)

i made a LC last week (after reading this tek) with a 1/2 pint wide mouth canning jar. i have a PC so i just boiled some water for a while filled the jar with that added some honey and a small piece of a paper clip for a stirring rod. i then PC'd if for about 20-25 min. let it sit overnight then injected a half syringe of spore solution on accident. the reason that i used a half syringe (about 5cc) was because when i made the hole for the syringe needle in the lid i sealed it with silicone so when i inserted the syringe it caused a vacuum and sucked in the content of the syringe by itself. OK so after a week of it mixing at around 80 degrees nothing really happened the water still had a yellowish hue and there was minimal growth. was it because i used to much spore solution of that the jar was air tight? i have since made a second hole in the lid that is about a 1/2 inch big and silicone a piece of tyvek over it as a breather hole. i haven't started the the process of making the new LC until i get some feedback though and the syringe that i used was good i used it in a previous successful grow... thanks in advance...


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