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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #18985707 - 10/16/13 11:24 AM (10 years, 3 months ago)

Good god this tek has Fail with a capital F written all over it :smbfacepalm:


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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #19261970 - 12/11/13 06:51 PM (10 years, 1 month ago)

Quote:

cronicr said:
Quote:

loki44 said:
:ohboyohboyohboy:

How is that I didn't see this thread before?  hahaha 

:hahyeahwoo:



well it's seven years old and
Quote:

cronicr said:
spores to lc is the worst route to take IMO



:whathesaid:



:whathesaid:

This part gives me chills

Quote:

_OttO_ said:
Flame sterilise or alcohool swab the tip of the needle then peel back the foil on the jar to expose the small hole you made in the lid. Insert the syringe and inject 2CC of spore solution -



Replace the foil.




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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
    #19834836 - 04/12/14 08:55 PM (9 years, 9 months ago)

I love LC for exotics and right now have 5 on the go. But LC needs to be done under strict sterile conditions and to me things like using ms solution and having open inoculation holes in a lid sound so sloppy. Not using an SAB for that is even worse. I use lids with SHIP and whatman filters,  inoculate with clean agar wedges and use 60 cc syringes to cutndown on the number of times the SHIP needs to be pierced.

I have had success with LC. I have also had fail. I learned quickly that the difference between the two was proper sterile tek, and fortunatly I had agar to teach it to me. The problem I have with LC is the notion that they are a shortcut.  They are a powerful tool that needs to be treated with respect.


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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
    #19835196 - 04/12/14 10:13 PM (9 years, 9 months ago)

I got no problem with people asking questions.  If I jumped on someone for simply asking a question I apologize. But I make no apologies for calling out a tek which advocates using spores to inoculate, having holes exposed or opening a lid in open air. These things might have been considered an acceptable practice ten years ago, but we know better today :shrug: I also dislike the notion that LC is "noob friendly". Agar is noob friendly.  If you can get agar down then you are ready for LC.
:2cents:


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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
    #19835413 - 04/12/14 11:05 PM (9 years, 9 months ago)

This hobby is constantly evolving. Mycology is a relatively new discipline. Information from 10 years ago might as well be a cave painting today. Ten years ago we didn't know that:

1) Mushrooms need FAE more than RH

2) Light on a 12/12 schedule is beneficial at all stages

3) Coir can be used as a substrate, it was thought to be good for casing only

Today everyone grows on coir, no one would think to fruit in the dark and most common FC designs are more concerned with FAE than RH. No matter what, Open air inoculation of sterile media is a terrible idea. The end.


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Re: A *beginner friendly* method for making Liquid Culture [Re: bodhisatta]
    #19838789 - 04/13/14 04:33 PM (9 years, 9 months ago)

Thanks for the testimony van der greigen, that is exactly what I am talking about. Proper sterile tek is not hard, like you said a cardboard box & saran wrap are not hard things to come by and will save a lot of grief. Ditto for the rest of the issues that have been mentioned. Noobs thinking proper sterile tek is hard is easily fixed. Growing nothing but contams will turn people off the hobby quicker.

Far more contam issues on the boards are traced back to LC than for agar. If someone asks me if agar is hard I will tell them no, its much easier than LC. I then will be satisfied that I gave them good info.


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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #19841770 - 04/14/14 05:46 AM (9 years, 9 months ago)

I might not be a TC but, I can't believe what I am reading. Open air with sterile media is not a good idea.


Quote:

FrankHorrigan said:
I will say that open air inoculations of anything except pasteurized substrate with fully colonized grain spawn contams on me in days, no exceptions.




http://www.shroomery.org/forums/showflat.php/Number/18484893#18484893

Quote:

FooMan said:
Do you live in a bubble? Advising people to do agar or any other type of sterile work in open air is just ridiculous. Any serious mycologist knows better.




http://www.shroomery.org/forums/showflat.php/Number/5741264#5741264


These are just 2 I pulled from a quick search which yielded hundreds of hits :shrug: As for open air agar, this guy found what happens when you expose a plate for just a few seconds in open air. He was working in a very still and sanitized environment, and had gotten away doing G2G like this. Was good enough to post his findings

As for spore syringes from trusted sources, they too are not infallible. I have had contams in spore syringes on occasion and when I emailed the vendor they merely asked for the lot number so they could test the batch, then replaced the syringe. They did this because they know that syringes are not always clean. Firing 2 cc of ms solution into a LC is risky IMO. It would only take a quick search to find hundreds of posts where you can obviously see the source of contam in a grain jar or pf cake was directly from the inoculate.

Like I said, I like LC. But it needs to be done with a certain level of care and attention to sterile tek. I won't beat this horse any longer. However I will direct any people with issues due to this tek to your thread 13, hope you won't mind.


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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_]
    #19845211 - 04/14/14 06:45 PM (9 years, 9 months ago)

Nice looking fruits OttO :thumbup: I can't say I wholeheartedly agree with your methods but, there is no denying that they work well for you. I'd be a hypocrite as well if I said I never did anything that was outside the recommended norm, shit I sterilize my coir/verm sub on a regular basis and am considering going back to the bucket as well. Guess I just love me agar too much, and I hate the fact that people think its hard. If it wasn't for agar, I'd probably still be doing the pf tek :lol:


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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
    #19846563 - 04/14/14 10:51 PM (9 years, 9 months ago)

Quote:

DeadHearts said:
:thisisterrible:




Really? Someone stressing sterile tek is facepalm worthy?  My god people, the goal is to grow mushies not to see how much we can half ass shit and get away with it :shrug:

I don't want to stir this shit any more than I have but seriously this hobby is not about doing as little as possible.


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Re: A *beginner friendly* method for making Liquid Culture [Re: DeadHearts]
    #19846721 - 04/14/14 11:38 PM (9 years, 9 months ago)

Quote:

DeadHearts said:
But do not deny open air teks do not work and work well because they do :lol:




I will say that it is possible that open air teks work. It is also possible for me to win the lottery. What I have been saying all along, is that I like my advice and/or recommendations to be based on a certain level of certainty. A clean agar wedge is clean. Anything inoculated in open air is quite possibly not. Spore prints from mushrooms fruited in open air are sometimes not. Syringes made with those prints are sometimes not. A clean agar wedge is still clean, I can tell by looking. An LC can be contamed and I might not know until I grow a pile of nasty.

I like clean LC. I hate contamed LC. I would rather have LC that I know has the best chance to be clean, than have one that someone thinks has a good chance of being clean. I don't care if one is perceived to be easier than the other. If anyone thinks agar is hard, then maybe they should try finger painting.

Sorry OttO, I am now done.
*hides thread*


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Re: A *beginner friendly* method for making Liquid Culture [Re: spacechildo]
    #20649549 - 10/02/14 04:32 PM (9 years, 3 months ago)

To be honest my biggest issue isn't even using spores to inoculate,  I have had bad luck with syringes but that's not to say everyone will. Its not even the open air inoculation, some people gave gotten lucky with open air g2g etc. No my problem is the unfiltered hole in the lid with nothing but some foil over it. If all we need for GE of any sterile media is a hole and some foil then why the hell do we bother with dry verm layers for pf tek or SFD's and polyfil for grain jar lids. Why would so many people have bothered to write teks on these things when all we need for 95% success is a hole and some foil :shrug:

Personally I think that anyone who advocates something like this had better use it themselves. Not used it once or twice and got away with it, they should be using it everytime cause its easier and if it works as well as they claim then making proper grain and LC lids would just be a waste of time and money.

I'm done. *hides thread*


Edited by Pastywhyte (10/02/14 05:04 PM)


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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20762991 - 10/28/14 08:58 AM (9 years, 3 months ago)

Use a big syringe. I like 60cc myself. Fill 4 of them up, test each and then good to go.


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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20763185 - 10/28/14 10:01 AM (9 years, 3 months ago)

Agar is a pretty fast way to check. Brf and grain jars are ok too but I like agar as its really easy to spot anything not right. If you go with agar do a few plates tho just to be sure.


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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20763287 - 10/28/14 10:29 AM (9 years, 3 months ago)

If you do ten plates and all ten turn out good, I would say your good. Otherwise your just rolling dice.


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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20763333 - 10/28/14 10:40 AM (9 years, 3 months ago)

Depends on a few things. Mold spores and bacterial endospores take time to germinate. Even after they do it will take them sone timw to take over. If you have a few endospores from poor sterilization or bad inoculation practices its possible thqt you could have a few come up clean. A week later tho while your test plates are growing out could mean the entire LC is comprised.  There is no mostly clean with LC. Either it is and every test is successful, or it isn't.


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Re: A *beginner friendly* method for making Liquid Culture [Re: stareatclouds]
    #20763355 - 10/28/14 10:48 AM (9 years, 3 months ago)

LC has lots of upside just be smart how you use it :thumbup:


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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20803539 - 11/06/14 09:02 PM (9 years, 2 months ago)

Honestly I like LC. I think the reason I take issue with LC from spores is simply because of the number of contamed syringes I have gotten from vendors (even got a couple from sponsors). My own prints have always turned out clean and so I probably could pull off a spores inoculation with prints I made myself. But most noobs won't be using prints they made themselves and so I shudder at the prospect of them having the same bad luck I had.

There is only one sponsor I would trust to make LC from spores with. Fact is I already did that and it was fine. Maybe I just have bad luck. But it has made me wary, which is not always a bad thing.

My last cube LC was a purple mystic clone and that shit was aggessive as hell, a thing of beauty to combine those genetics with LC :rockon:


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Re: A *beginner friendly* method for making Liquid Culture [Re: cronicr]
    #20803613 - 11/06/14 09:16 PM (9 years, 2 months ago)

My first time dunking grains had me sold, was 4 days to colinize and five days after that I had



Snap eat ya always got some great stories, fucking great stuff :rockon:


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Re: A *beginner friendly* method for making Liquid Culture [Re: eatyualive]
    #20803697 - 11/06/14 09:33 PM (9 years, 2 months ago)

Just a light duty trash bag for bathroom garbages. Just something to keep the tray intact :lol:


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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #20807901 - 11/07/14 10:14 PM (9 years, 2 months ago)

Once I had a jar with the top grains all taken over by penicillin that worked through the filter.  Just scraped them off outside, brought it back in and spawned. Was one of my best looking trays ever, I shoulda taken pics :facepalm:


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