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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: DJ Lance] * 1
    #17352349 - 12/07/12 08:08 PM (11 years, 1 month ago)

Quote:

DJ Lance said:
Wow, this is really helpful. With all of the things I'm trying to study and learn and read at the same time, this really relieved a lot of my stress!



This tek sucks imo.

LCs from spores are sketchy. No spore syringe/print is ever 100% clean.

This is because the cap from which the spores came was more than likely fruited in the open air.

You cannot know 100% if an LC is good or not by visual inspection.

Test jars must be shot up and grown out.

Conditions are not correct for germination of spores in sugar LCs, grain LCs or extra light malt extract based LCs are perfect for spore germination.

For best results, make LCs from a fully colonized grain jar(GLC) or from an agar wedge.

Or skip LC entirely and just G2G(Grain to Grain) transfers to expand your mycellium.


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz] * 1
    #17352756 - 12/07/12 09:51 PM (11 years, 1 month ago)

Quote:

Rahz said:
Quote:

Conditions are not correct for germination of spores in sugar LCs




My spores seem to like honey a lot.



:shrug:


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #17536088 - 01/12/13 02:54 AM (11 years, 18 days ago)

Quote:

_OttO_ said:
Fear based posts like this prevent people from playing with this hobby.



Fear based? LOL!

It's a known fact that LCS have a higher failure rate than G2G transfers.

It's also a known fact that LCs from spores are sketchy.

Also sugar based LCs are not the proper environment for spores to germinate, there are better methods.

If this scares noobs away from LCs, well then I think I did good, and I am only considering the massive failure rate that they could have when compared to G2G transfers.

Plus G2G is a faster and safer way to expand mycellium.

God forbid I teach noobs about a more successful way of doing things.

Quote:

_OttO_ said:
so go suck a cock. :smile:



No Thanks.


Edited by PussyFart (01/12/13 02:56 AM)


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OfflinePussyFart
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Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz] * 1
    #17537040 - 01/12/13 10:59 AM (11 years, 17 days ago)

Quote:

Rahz said:
Quote:

Also sugar based LCs are not the proper environment for spores to germinate




I pointed out earlier in this thread that this comment was odd. Spores germinate fine in various sugar solutions. You might want to provide some rational info to back up your claim, otherwise you just seem biased. In this case 'proper' seems more like a (wrong) opinion on your part.



Just something a TC posted in one of my threads...Not to mention all the sugar(honey) based LCs I have made from spores have failed, but the 2 I have made with ELME from spores I am having some success with.

http://www.shroomery.org/forums/showflat.php/Number/17167323

Ok, so even if you take that one statement away, there is still a higher failure rate when compared to other methods of expanding mycellium.

And in my opinion and experience, G2G is a far less risky move, especially for noobs, so why not start them off on a far more successful road then LCs from spores?

It's been my observation over the last 6 months that LCs are becoming less and less popular because of all the failures, and the search results speak for themselves.


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Re: A *beginner friendly* method for making Liquid Culture [Re: _OttO_] * 1
    #17540041 - 01/12/13 10:11 PM (11 years, 17 days ago)

I hear ya...and I never meant to offend you, I guess I never should have said it sucked, sorry.


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Re: A *beginner friendly* method for making Liquid Culture [Re: odbsmydog] * 2
    #17791808 - 02/12/13 07:45 PM (10 years, 11 months ago)

Check out TL's G2G tek in my signature.


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Re: A *beginner friendly* method for making Liquid Culture [Re: odbsmydog] * 1
    #17794109 - 02/13/13 07:52 AM (10 years, 11 months ago)

I have used it as a referencepoint, on some occasions, but the way I do it differs a little from the way TL does it.

First off the lid to the master jar stays off, he likes to put it back on after every jar, which is fine, but I see it as a couple seconds I can save.

I hold the master jar in my right hand and pour it the same way he does, but I usually only use 1 hand to close the receiving jar...now sometimes I have to use 2 hands, but not always. Before I start IO loosen every jar lid almost completely, so i can twist it a little with my left hand, tilt it so I get the ring and the lid to pop off, pour the grains and seal it back...I can do 10 jars like this in about 5 minutes, once I get started.

Just small little differences to get to the same outcome.


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Re: A *beginner friendly* method for making Liquid Culture [Re: PussyFart]
    #18907459 - 09/29/13 02:58 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
You cannot know 100% if an LC is good or not by visual inspection.

Test jars must be shot up and grown out.




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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
    #18985453 - 10/16/13 10:09 AM (10 years, 3 months ago)

Nope....maybe spores.....maybe a contam....no way to know for sure.


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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
    #18985581 - 10/16/13 10:52 AM (10 years, 3 months ago)

Quote:

wyatteb said:
Do the spores expand like that before myc growth?



Not really.

Quote:

wyatteb said:
Guess I should wait er out a few more days before I decide to toss.



Why would you toss it after only 3 days?

Spores have been known to take 2+ weeks to germinate and show growth.

Then you have to wait for the LC to grow out, then shoot up test jars...

Quote:

wyatteb said:
The is my first lc by the way



And I hope it will be your last, for your sake.

Spores are by far the worst way to start an LC.


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Re: A *beginner friendly* method for making Liquid Culture [Re: wyatteb]
    #18985691 - 10/16/13 11:20 AM (10 years, 3 months ago)

Quote:

wyatteb said:
Wow dude if you read the original post then you would see that this teks lc is said to be ready in about a week and has spore injection as one of the options. I followed this tek because it was easy and I had limited supplies. No need to be rude



I was not rude at all.

I simply stated facts.

I am sorry if you took it personal.

I was sincere about that last part, there are better ways than messing with LCs, especially LCs from spores.

I suggest researching G2G and spawning to bulk if you really want to expand your mycellium.

Not talkin shit about the tek, just being real.


Edited by PussyFart (10/16/13 11:21 AM)


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Re: A *beginner friendly* method for making Liquid Culture [Re: hypnofrog15]
    #19060800 - 10/30/13 10:28 PM (10 years, 2 months ago)

LCs from spores are sketchy as it is.

Adding tissue just adds more vectors for contams.


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Re: A *beginner friendly* method for making Liquid Culture [Re: hypnofrog15]
    #19061068 - 10/30/13 11:17 PM (10 years, 2 months ago)

Sure you can, but why not just use the tissue?

Why even use spores?

The spores will take at least a few days to germinate and show growth, but the tissue is live mycellium.

It will start growing right away.


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Re: A *beginner friendly* method for making Liquid Culture [Re: azur]
    #19066676 - 10/31/13 10:26 PM (10 years, 2 months ago)

Quote:

azur said:
I'm the biggest fan of liquid cultures. I've had two out of over a hundred contaminate and I was able to see those in the liquid. I find them to be much easier and faster than g2g.



By the time your LC colonizes and your test jars are shot up and grown, I can have 100 quart jars just about fully colonized in the same amount of time via G2G transfers.

LCs save no time....it just adds another step and more risk of contams.

I will never touch a syringe again....agar and G2G FTW!


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Re: A *beginner friendly* method for making Liquid Culture [Re: SuperLuigi]
    #20379339 - 08/05/14 05:12 PM (9 years, 5 months ago)

Quote:

SuperLuigi said:
I have tried an LC and it went clear and has some lil sphere that is about the size of a Q-tip head on it.  It's been almost 2 weeks and it hasn't gotten bigger.  Is that unusual?



:shrug: Test it!


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Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #20379587 - 08/05/14 06:18 PM (9 years, 5 months ago)

Quote:

Rahz said:
I would assume a contam and start again.



Ahh yes.....gotta love that LC......besides being a complete waste of time, what is the point?


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Re: A *beginner friendly* method for making Liquid Culture [Re: Rahz]
    #20380661 - 08/05/14 10:35 PM (9 years, 5 months ago)

Quote:

Rahz said:
Quote:

Ahh yes.....gotta love that LC......besides being a complete waste of time, what is the point?




It's an easy way to culture multispore. I've been using it for years -w- a silicone injection port and polyfill stuffed vacuum tubing for air exchange. Works great.



Sure it works great, when it doesn't contaminate.

Agar is better...you should graduate. With agar you can transfer away from contams...this makes it far superior to LCs....

And since no print or syringe is ever 100% clean, and LCs are the perfect breeding ground for everything not fungi related, I would say it's a piss poor method of culturing MS.....but that's just my opinion....I like a high success rate with as low of risk as possible, as should most cultivators.


Edited by PussyFart (08/05/14 10:39 PM)


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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647538 - 10/02/14 06:06 AM (9 years, 3 months ago)

Basically......but making an LC from spores is by far the worst way to start an LC.


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Re: A *beginner friendly* method for making Liquid Culture [Re: FilthyShroomz]
    #20647608 - 10/02/14 06:33 AM (9 years, 3 months ago)

No print/syringe is ever 100% clean, as the cap was most likely fruited in open air.

LC is the perfect breeding ground for everything nasty.

If you are going to make an LC, for the best success rate, use a clean agar wedge.


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Re: A *beginner friendly* method for making Liquid Culture [Re: 13shrooms]
    #20647870 - 10/02/14 08:56 AM (9 years, 3 months ago)

Quote:

13shrooms said:
you guys need to stop answering with your negitive bias against LCs, if you dont like them fine but dont discourage..  :



No one on this page was bias......cron said something about LCs not being needed for cubes, and the success rate is not right for him(and I agree, because the success rate is not that good when coming from spores)....

Then I said spores are by far the worst way to start an LC.....which is a fact.....I also said that making one from an agar wedge will give you the best chance of success....which is a fact.

What part of that was bias?

Quote:

13shrooms said:
my point is yes there are "better" or higher success rate beginner teks for all kinds of things like a flow hood is better than a glovebox or SAB BUT we dont tell others not to use them just how to do it correctly.  do you guys get the difference??  :wow:



A flow hood is not "Better" than a SAB....I have 100% success rate in my SAB....a flow hood would not improve that.....they are on the same level of effectiveness when used correctly.


Edited by PussyFart (10/02/14 08:58 AM)


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