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Offlineblackout
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Starter LCs in small containers.
    #5220794 - 01/25/06 06:32 AM (18 years, 25 days ago)

I am trying out starter cultures for LCs. The idea is injecting spores into small amounts of LC, they should not get lost in the large volume and so find a mate quicker. I injected 1/4cc of SA in 2.5ml of LC and 2 days later had growth. I was able to shake very hard in the vacutainer type bottle. Next I will inject 1ml into a large 750ml jar.
A better test would be to do 1/4cc in say 10 starters and 10 in big 1 litre jars. After the starter is visible inject it into a new litre jar of LC. Then after a week or so compare results, I think the ones from starters will be ahead. I have had little success with large jars, most advise to add more spores to larger jars. In commercial setups they use starter cultures, same in brewerys using starter yeast cultures, so do some homebrewers.

The vacutainer type tube was laid on its side so it is well exposed to air, I think this is also important, a large liquid to surface ratio. Now I can take out my 1ml and store the small container in the fridge.


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InvisibleMrMaddHatter
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Re: Starter LCs in small containers. [Re: blackout]
    #5220827 - 01/25/06 07:05 AM (18 years, 25 days ago)

:thumbup: Sounds like a good idea.


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