Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblemikonn
me

Registered: 05/29/05
Posts: 192
New to Microscopy and looking for some help...Pics...
    #5211427 - 01/22/06 06:34 PM (18 years, 3 months ago)

I've been lurking around here for a while and I hope this is the right place for this post. If there was an introductory mycology forum it would probably go there. So I found an interesting specimen last summer, the original post is here
I've been working cloning it from perioxided section of the stem (it's finally coming along, and I'll maybe post some picture for questions later). But while I'm doing that, I've been trying to ID some of the microscopic features with a few of the specimens that I have. It's sorta hard, as the caps a particularly small and the gills are really really small. I don't have anyway of doing any measurements at the present time, so I understand that really IDing the species is pretty much out, but I've been trying to document the various features that I can find and try to figure out what they are. So here they are...

Here are a picture of the spores and some unknown little squiggly things.

Here a farther away shot of a gill and a gill edge with some squigglies.

Cap Cuticle Cellular

Basidia is four-spored.

Some cheilocystidia (I think...)

Here is an interestingly shaped cell.

Here are some yellowish cells that I found on the cap.

Now here is what appears to be some sorta of cells that are on the gill face I don't know if they are pleurocystidia or something else.

Here is a picture of the edge with shows off the whitening effect caused by the cheilocystidia.

Lastly here are some contaminents that where hiding in the cap.

So thanks so much for looking and please let me know if I've totally screwed up on anything or everything.

Extras: Filter Print Post Top
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5232090 - 01/27/06 04:59 PM (18 years, 3 months ago)

VERY NICE microscopy work.

Keep them coming.

Since you invested in a scope you should really invest in a ocular micrometer and a stage micrometer to calibrate. You can get the two brand new for around a hundred bucks or so. Go with the .001 size micrometers. :thumbup:

Edited by EonTan (01/27/06 05:11 PM)

Extras: Filter Print Post Top
InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
 User Gallery
Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5232135 - 01/27/06 05:10 PM (18 years, 3 months ago)

That is very nice, what kind of microscope/camera did you use?


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

Extras: Filter Print Post Top
OfflineCubenisseur
Mad Props
Male

Registered: 12/04/05
Posts: 1,392
Loc: Indian Land
Last seen: 14 years, 1 month
Re: New to Microscopy and looking for some help...Pics... [Re: Psychoslut]
    #5233184 - 01/27/06 09:52 PM (18 years, 3 months ago)

I'm seeing all sorts of shit in those pictures! Cool stuff.

Extras: Filter Print Post Top
OfflineWorkmanV
1999 Spore War Veteran
Male User Gallery

Registered: 03/01/01
Posts: 3,604
Loc: Oregon, USA
Last seen: 41 minutes, 28 seconds
Trusted Cultivator
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5238411 - 01/29/06 12:33 PM (18 years, 3 months ago)

Looks great for your first attempt. Eontan is correct that you need to be able to measure the structures.

I am interested in the photo editing software or technique you use to outline the structures. I have done it by hand and its not as crisp as yours.


--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply

My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification :amanitajar:

Extras: Filter Print Post Top
Invisiblemikonn
me

Registered: 05/29/05
Posts: 192
Re: New to Microscopy and looking for some help...Pics... [Re: EonTan]
    #5239056 - 01/29/06 03:40 PM (18 years, 3 months ago)

Thanks alot. It's been fun. As soon as I get a little extra money, I'm going to get the micrometers. EonTan, do you have any suggestions as to a reputable supplier online? Workman, the outlines were done in Photoshop using the pen tool, I simply used it to create the path then stroked the path at 2 pixels. I also used Photoshop to composite 5 pictures of varying focus in the picture of the gill edge, though it's still not very much in focus.

As for the structures shown, do they appear to be what I've labeled them as? What is the yellowish cell on the cap? Any thoughts? Also is anyone familiar with a species that has similiarly shaped cheilocystidia, they have the shape very much like a duckpin bowling pin. Like so...


Also, I've been noticing that there doesn't seem to be many pictures online of various species microscopic characteristics. The Shroomery, meaning it's members, should try to cull together a collection which could act as a reference for id and educational purposes. It seems that it would be an incredibly value tool. Perhaps a Microscopy section of the wiki? Just a thought.

Extras: Filter Print Post Top
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5239552 - 01/29/06 06:29 PM (18 years, 3 months ago)

Cystidia can be found growing from the cap surface, Pileocystidia. Those found from the stem surface are Caulocystidia. The ones from the gill surface are Pleurocystidia and Cheilocystidia(edge). Endocystidia are found inside the trama( stem, cap, or gill).

If you are really into this stuff Get the book HOW TO IDENTIFY MUSHROOMS TO GENUS 3: Microscopic features. By largent,johnson, and watling.

Cynmar.com not a sponsor sorry guys( don't think any sponsor carrries these anyway). Has many ocular micrometers and a stage micrometer.

Extras: Filter Print Post Top
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5239633 - 01/29/06 06:50 PM (18 years, 3 months ago)

lecythiform is the bowling pin type cystidia.

variations of this shape are common. Lageniform is one type(pear shaped).

To me your unknown is similar to the unknown Panaeolus we get here on new turf. Turf originating from the Everglades regions. Unfortunately some new St.Augustine sod is being used frequently where I live, and it is full of mycena and conocybe, not panaeolous.

Extras: Filter Print Post Top
Invisiblemikonn
me

Registered: 05/29/05
Posts: 192
Re: New to Microscopy and looking for some help...Pics... [Re: EonTan]
    #5240005 - 01/29/06 08:28 PM (18 years, 3 months ago)

Thanks alot.
I found this pretty good reference for beginners.
here
It was linked to at sporeworks.

I wish I could find something like that with pictures. I quess I'm more of a visual learner...

EonTan, do you know of any posts pertaining to the unknown you are refering to? Just curious. It would be interesting to find out where the place around here got their turf from...

Extras: Filter Print Post Top
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5240183 - 01/29/06 08:57 PM (18 years, 3 months ago)

Search Paneolus ggreatoneifolius. Ggreatone was the first to post about these on the OMC. There have been many posts about the unknown Panaeolous growing from new and old turf in florida. Many members have found it.  Search mushroom hunting forums and unknown panaeolus.

I think workman grew one recently and labled it a Petite lawn Panaeolous subbalteatus.

I don't think it is a subbalteatus. Its microscopic features are not consistent with subbalteatus, or any other Pan I have seen in textbooks, or in my hands.

Paneolus acuminatus
Panaeolus subbalteatus
Panaeolus castaneifolius
Panaeolus ggreatoneifolius :smile:

It is a mushroom that is not refrenced in any english Language text
I can find. I have seen it mislabled by mycologists in the Southern Mushrooms field guide, as acuminatus because it was blackspored and the spores were smooth, growing from grass with no visible manure present in the soil.

Extras: Filter Print Post Top
Invisiblemikonn
me

Registered: 05/29/05
Posts: 192
Re: New to Microscopy and looking for some help...Pics... [Re: EonTan]
    #5243883 - 01/30/06 08:21 PM (18 years, 3 months ago)

Thanks EonTan.
Just to clarify, the pictures here are from different type of unknowns than the ones you refer to. I believe you are refering to these
which were from a specimen that was collected in Maryland (there is a link at the beginning of his post to my original post). These are quite different(see link at top of post), at least macroscopically, but who knows. I am continuing to try to figure them out.

Extras: Filter Print Post Top
InvisibleEonTan
bird
 User Gallery
Registered: 08/18/04
Posts: 468
Loc: very south
Re: New to Microscopy and looking for some help...Pics... [Re: mikonn]
    #5260223 - 02/03/06 06:15 PM (18 years, 3 months ago)

Yes the macros are confusing. Microscopically they look pretty close.

I am referring to BOTH collections YOU posted. Microscopically both of your collections look similar to the Florida finds. MAcroscopically, I have had collections that look closer to the maryland finds. The incurved margin, is what really looks different to me!!!

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals Bridgetown Botanicals   Kraken Kratom Red Vein Kratom   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Substrate Bags   MagicBag.co All-In-One Bags That Don't Suck   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale


Similar ThreadsPosterViewsRepliesLast post
* Micrometer ideas? Joshua 1,433 12 04/13/04 12:47 AM
by Joshua
* Lowest power on microscope? VividDreams 1,754 11 10/17/03 08:00 AM
by micro
* more samui (pic) B.I.O. 1,535 8 11/07/01 09:49 PM
by ralphster44
* Cyans Trops? w / pics FungusmaximusFM 1,199 8 07/26/03 09:21 AM
by Zen Peddler
* Will this stage micrometer work for measuring spores? BlimeyGrimey 811 3 03/31/09 10:10 AM
by fastfred
* Microscope recommendations anyone? armedia 2,465 6 04/24/02 05:20 AM
by zeronio
* stage micrometer blackmold 993 4 08/29/04 03:01 PM
by ragadinks
* micrometer calibrator Zen Peddler 1,084 6 09/06/06 03:35 AM
by Zen Peddler

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
2,132 topic views. 1 members, 2 guests and 1 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.019 seconds spending 0.004 seconds on 12 queries.