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OfflineNESpores
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Cloning PE For Spores
    #5209926 - 01/22/06 11:33 AM (18 years, 3 months ago)

quick qeustion here guys, would this work fine?
I traded a spawn bag for a PE print in a vacutainer, and I plan to make live culture from it.
Im goint to inoculate like 10 .5pint jars of wbs to spawn to cow poo compost.
When they fruit, I will look for any that sporulate, and select the biggest, clone it, and make live culture of that.
Doesn't that solve the problem of not getting many spores from PE?
I haven't heard of any body else doing this before... will i be the first?


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Offlineblackout
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Re: Cloning PE For Spores [Re: NESpores]
    #5209952 - 01/22/06 11:39 AM (18 years, 3 months ago)

Never heard of it either. It makes sense. Not sure it will guarantee they will all be spore depositors, but it makes more sense than cloning one that doesnt drop spores, if that is what you are really after (i.e. there may be good characteristics in another that doesnt drop spores)

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OfflineWronguy
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Re: Cloning PE For Spores [Re: blackout]
    #5210011 - 01/22/06 11:53 AM (18 years, 3 months ago)

I'm not sure what kind of responses you'll get in here NESpores. If you'd like, I can move this into the Advanced Forum. Most of those guys know quite a bit about cloning.

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OfflineNESpores
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Re: Cloning PE For Spores [Re: blackout]
    #5210025 - 01/22/06 11:57 AM (18 years, 3 months ago)

i didn't explain well enough.
Ill let the first batch mature, and find one good sized SPORULATING MUSHROOM. then clone it, and grow a big batch, and in theory, all of those mushrooms will also sporulate.
i want to be able to make a but load of spore ring donations. Its taken me months to get these spores and I think anybody should have access to them.

please move this to advanced cult


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Edited by NESpores (01/22/06 12:00 PM)

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Offlineblackout
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Re: Cloning PE For Spores [Re: Wronguy]
    #5210028 - 01/22/06 11:57 AM (18 years, 3 months ago)

On second reading of that I think I picked it up wrong. If you are going to just clone to keep it going (I think many do with PE) then there is no need to specifically pick a sporulating one, pick the best one like I mentioned before.

I thought you were cloning a sporulating shroom in the hope that the future shrooms from that isolate will all be sporulators too.

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OfflineWronguy
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Re: Cloning PE For Spores [Re: NESpores]
    #5210042 - 01/22/06 12:01 PM (18 years, 3 months ago)

Quote:

NESpores said:
please move this to advanced cult




You got it.

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OfflineNESpores
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Re: Cloning PE For Spores [Re: blackout]
    #5210043 - 01/22/06 12:01 PM (18 years, 3 months ago)

"cloning a sporulating shroom in the hope that the future shrooms from that isolate will all be sporulators" is exactly what i plan to do

anybody over here in the advanced form have any ideas/comments/raves?


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Edited by NESpores (01/22/06 12:04 PM)

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OfflineWorkmanV
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Re: Cloning PE For Spores [Re: NESpores]
    #5215012 - 01/23/06 06:41 PM (18 years, 3 months ago)

In my experience, all PE strains from spores are pretty uniform with limited genetic variability. This is from repeated subculturing from spores which reduces variability by half with each subsequent generation. I doubt you will find a PE strain that sporulates much better than any other from a multispore culture. Also, the strain is a poor sporulater mostly because of its structure (reduced cap size, swollen stem that covers the gills and overly crowded gills). Actual spore production isn't that bad, as indicated by the darkening of the gills, its just that the spores don't drop that well from the malformed cap (which is the main attribute of the strain). So, even if you could select out a heavy sporulating PE strain, if it didn't have the penis shaped cap noone would be interested.

That said, your method would be the correct one, especially for wild strains with maximum genetic variability still intact.


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Offlineonetime
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Re: Cloning PE For Spores [Re: Workman]
    #5215917 - 01/23/06 10:21 PM (18 years, 3 months ago)

ralphsters is selling what they called PE #6 or something like that they really don't look much like the typical PE's but are said to generate more spores. I guess it was made by roger rabit.

http://www.ralphstersspores.com/index_files/Page3940.html



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Invisiblemycogirl
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Re: Cloning PE For Spores [Re: onetime]
    #5222777 - 01/25/06 06:15 PM (18 years, 3 months ago)

Also, from what I understand...it would depend on whether sporulating is genetic or occurs as a result of environmental cues... I don't know enough about this particular species though to know.


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OfflineRogerRabbitM
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Re: Cloning PE For Spores [Re: mycogirl]
    #5224480 - 01/26/06 12:56 AM (18 years, 3 months ago)

PE6 is a Tex/PE cross, and unfortunately the 'texas' shape presents itself much more than the PE. I agree with workman in another thread when he said the penis shape and albino coloration are recessive.

PE6 shouldn't be considered a true PE because of this.
RR


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Offlineblackout
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Re: Cloning PE For Spores [Re: Workman]
    #5224836 - 01/26/06 03:29 AM (18 years, 3 months ago)

Quote:

Workman said:the spores don't drop that well from the malformed cap



Is there another way? I have heard of cutting the caps lenghtways. Could you dump the cap in sterile water and shake it will to draw them out?

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Offlinegourmetgrower
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Re: Cloning PE For Spores [Re: blackout]
    #5240122 - 01/29/06 08:48 PM (18 years, 3 months ago)

You could do a spore dilution and isolate dikaryons to find multiple sporulating strains. The Mushroom Cultivator describes the process in detail, in the Appendix somewhere.

You dilute a small sample of the print in sterile water. Then, depending on the concentration, you dilute 2+ more times. Put a few drops on some petris. (I have overdiluted when doing this - no growth)

Basically, some uncompatible spores will not join, and some will. The ones that do are sterile. When you isolate the dikaryons, you can grow out, say, a dozen or 20 colonies (whatever a stack of your petri brand is). Fruit them either from a bag or cake, or even fruit from your agar if it is nutritionally sufficient.

When you have sporulating strains, you can take samples of the mushroom stems and propogate them on petris, and this will give you multiple (sub)strain cultures that are not sterile, and should theoretically fruit.

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Invisibleshymanta
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Re: Cloning PE For Spores [Re: RogerRabbit]
    #5355672 - 03/01/06 11:00 PM (18 years, 2 months ago)

From what I've read, PE has a distinctive trip (less wave like). ralphstersspores.com says: "... Not quite as phallic looking as it?s original strain, but effectively similar."

Is effectively similar talking about the feeling they produce?

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