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OfflineMepher
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LC - how long is it good?
    #5201337 - 01/20/06 12:45 AM (18 years, 2 months ago)

How long is a liquid mycelium culture really good for? I've seen a few people say "use it within a few days", while some have said a couple of weeks up to a month. Is this accurate? Will it be good for longer if there are no visible contams?

What about two months if there are still many obvious strands of mycelium and nothing else apparent in the container? Just curious, as I certainly wouldn't want to use an out-dated or LC only to get huge contam rates.

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OfflineSnaggletooth
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Re: LC - how long is it good? [Re: Mepher]
    #5201352 - 01/20/06 12:53 AM (18 years, 2 months ago)

I don't now if there is a time limit given

I thought Agar said indefinitely...(of course it depends on how you store them like in a Vacutainer cool, dark place and stall the growth)

I have not seen study on long term storage of LC..I know that it could degrade over time...but that's still a viable sample..

I don't doubt Agar...longer then you would probably would need it... :grin:


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Offlinexaxphaanes
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Re: LC - how long is it good? [Re: Snaggletooth]
    #5201444 - 01/20/06 01:51 AM (18 years, 2 months ago)

i have used an lc that was stored in an quart jar sealed in a food sealer stored for about 3 months


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Offlineblackout
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Re: LC - how long is it good? [Re: xaxphaanes]
    #5201500 - 01/20/06 02:22 AM (18 years, 2 months ago)

Some have used them after a year in the fridge. Lately mine are all dying on me after only about 1 month at room temp. I now think it is best to use them 1/2 way to full growth, when there is still vigorous growth, thats when I have had my fastest colonisations.

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OfflineDSD
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Re: LC - how long is it good? [Re: blackout]
    #5201536 - 01/20/06 02:49 AM (18 years, 2 months ago)

good for a couple months if stored in the fridge, but the older they get the slower they are - but still good.

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