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goodoldrascal
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Registered: 01/14/06
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Disposable Glove Box- To HEPA or not to HEPA
#5193104 - 01/17/06 10:10 PM (18 years, 1 month ago) |
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Can a disposable glove box (or standard portable) be used w/o the aid of a HEPA filter? Would it be ok to use something like lysol to sterlized the air within the glove box before use?
-------------------- Recent Discovery: Check out the Tibetan Book of the Dead, relevant information, and esp. Leary's translation/interpretation. I hope you will be as astounded by this ancient texts' relevance to the psychedelic experience as I have been.
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BataviaVakereli
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Re: Disposable Glove Box- To HEPA or not to HEPA [Re: goodoldrascal]
#5193226 - 01/17/06 10:39 PM (18 years, 1 month ago) |
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I don't think this is an advanced question, but I'll answer you anyway. You don't need a HEPA. The purpose of a hepa would to allow dirty air from the outside to filter though and become clean on the inside. I use a HEPA in mine to allow a flow of positive pressure sterile air to keep contams out. If the box is well sealed, you can get away with just using lysol on the inside. That'll keep everything inside clean. Just make sure nothing else can get in after you get it clean.
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RogerRabbit
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Registered: 03/26/03
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Re: Disposable Glove Box- To HEPA or not to HEPA [Re: BataviaVakereli]
#5193935 - 01/18/06 02:18 AM (18 years, 1 month ago) |
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A filter on a glovebox will only cause problems. A sealed, still environment is what you want. Also, I never use lysol or anything else but soap and water to clean the glovebox. There is nothing 'sterile' about a glovebox. It is supposed to stop air currents.
When I go into the field to get wild specimens for cloning, I take clear plastic trash bags with latex gloves and a petri dish in each one. A clear trash or oven bag makes an excellent ghetto glovebox. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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goodoldrascal
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Registered: 01/14/06
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Re: Disposable Glove Box- To HEPA or not to HEPA [Re: RogerRabbit]
#5194477 - 01/18/06 10:16 AM (18 years, 1 month ago) |
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Thanks for the info guys. I hope my first experience using spawn bags turns out to be a good one. I am wondering cleaning can be done before the materials used to begin colonization are put in the glove box. As RR said, it is not intended to be a 'sterile' enviornment, it shouldn't be a huge concern.. right?
-------------------- Recent Discovery: Check out the Tibetan Book of the Dead, relevant information, and esp. Leary's translation/interpretation. I hope you will be as astounded by this ancient texts' relevance to the psychedelic experience as I have been.
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RogerRabbit
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Registered: 03/26/03
Posts: 42,214
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Re: Disposable Glove Box- To HEPA or not to HEPA [Re: goodoldrascal]
#5194837 - 01/18/06 12:16 PM (18 years, 1 month ago) |
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Wait. The glovebox itself need not be sterile because we don't dump our spawn out on the floor or walls of the glovebox.
However, any tools that come in direct contact with your crop need to be sterile. Wear surgical gloves and flame sterilize needles, scalpels, etc. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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ohmatic
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Registered: 02/28/04
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Re: Disposable Glove Box- To HEPA or not to HEPA [Re: RogerRabbit]
#5194879 - 01/18/06 12:38 PM (18 years, 1 month ago) |
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Quote:
RogerRabbit said: Wait. The glovebox itself need not be sterile because we don't dump our spawn out on the floor or walls of the glovebox.
you dont want it to be encrusted in dirt though ..
when i was using my box (before i blew it up ..) i used to give it a nice hot water wash before wiping down the ENTIRE internal surface with rubbing alcohol so sanitize the whole surface.
i simply hated the idea of ANY possible germ to fall down from the lid and enter a petri dish of mine for example, you can overall eradicate this possibilty but sanitizing the surfaces. peace ohm
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