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Offlinecokaznrebel
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Registered: 11/09/04
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Thermal Death
    #5176864 - 01/13/06 10:33 PM (16 years, 8 months ago)

I believe i had my incubation chamber set up at 84 degress, but just found out my thermometer was reading a little low and now its 89 degress with my new mercury one. They were incubating at that tempeerature for 4 days, and now i have made adjustments and they are now back at 84 degress. How much harm do you think I did to my jar?

Would it be ok to re-innolculate with fresh spores if they all suffered thermal death, assuming it was still sterile?


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Offlinexxanxx
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Registered: 12/14/05
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Re: Thermal Death [Re: cokaznrebel]
    #5176895 - 01/13/06 10:39 PM (16 years, 8 months ago)

They are fine, mine has been up to 96F and they just slowed down a little but did fine. I believe thermal death occurs at 106F(somoene correct me if i'm wrong?), for cubes.


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OfflineRazman
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Registered: 07/08/04
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Re: Thermal Death [Re: xxanxx]
    #5176911 - 01/13/06 10:41 PM (16 years, 8 months ago)

89 is nothing! dont worry about it..


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Offlinecokaznrebel
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Registered: 11/09/04
Posts: 328
Loc: Mile High
Last seen: 2 years, 29 days
Re: Thermal Death [Re: xxanxx]
    #5176923 - 01/13/06 10:43 PM (16 years, 8 months ago)

Only reason I am concerned is becuase i see no growth in the jars at all, they look the same as when i got them in the mail 4 days after innolculation.


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OfflineSnaggletooth
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Registered: 10/25/05
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Re: Thermal Death [Re: xxanxx]
    #5177147 - 01/13/06 11:24 PM (16 years, 8 months ago)

Quote:

xxanxx said:
They are fine, mine has been up to 96F and they just slowed down a little but did fine.  I believe thermal death occurs at 106F(somoene correct me if i'm wrong?), for cubes.





:thumbup:.....106


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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: Thermal Death [Re: Snaggletooth]
    #5177279 - 01/13/06 11:51 PM (16 years, 8 months ago)

84 is still too hot though. Drop it down to 80 and you'll have far fewer problems, even if it does take a day or two longer to colonize. Bacteria is encouraged at higher temps.
RR


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