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Offlineshroomer68
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liquid culture mix
    #5172670 - 01/12/06 05:46 PM (15 years, 1 month ago)

i was wondering what are all of the different mixes you can use for lc's and which ones the best...i have 5 cc of innoculant left which i want to inoculate 12 quart jars of birdseed with...i went to the grocery store, the drug store, and the health food store and none had any clue what malt extract was or where to get it. and i was wondering if dextrose is needed in the mix or just malt extract...anyone with any experience with LC's, please share your procedure on making an LC.


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InvisibleJaeger
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Re: liquid culture mix [Re: shroomer68]
    #5172676 - 01/12/06 05:48 PM (15 years, 1 month ago)

Malt extract and dextrose can be found at a Home Brew shop.
Do a search for a karo or honey water tek :smile:  Karo is easily found at the supermarket!
And you only need like 1 cc


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Offlinewillmafingerdo
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Re: liquid culture mix [Re: shroomer68]
    #5172834 - 01/12/06 06:14 PM (15 years, 1 month ago)

96 cc water 4 cc honey/karo (both can be found at a grocery store, and ppl report that karo w/ vanila helps out. just look in the baking section) and 1 glass marble.

there are a couple ways of doing this..

1. get your jar, place the marble in and honey/karo in then add the water mix it up so all the honey/karo is disoved and PC for 10 min at 15 psi. only thing is that if you PC for to long the sugars will carmelize and turn the liquid a brozish color.

2. (this is the way i use no problems w/ contams so far) put some water on to boil, more than the 96cc, and let it boil for a couple min. while thats going put the marble and honey/karo into your jar. mesure out the boiling water and add to the jar and shake. the jar will be very hot!! use oven mits or something like that..

hope this is what you were looking for.. and hope it helps

l8r,
will


--------------------
:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

"are you sure u want to eat all them shrooms??"  "yes i want to hear what my mind has to say..."

remember opions are like assholes, everyone has one and they all stink.


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Offlineshroomer68
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Re: liquid culture mix [Re: willmafingerdo]
    #5173067 - 01/12/06 07:06 PM (15 years, 1 month ago)

yea this honey/karo tek seems okay, i think ill give it a go. how do u get the exact amount of honey, karo and water though (96 cc - 4 cc)?


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Offlinexxanxx
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Re: liquid culture mix [Re: shroomer68]
    #5173155 - 01/12/06 07:26 PM (15 years, 1 month ago)

I like the Karo Tek myself, and I use Agar's baby bottle tek - thanks Agar for the excellent tek!!

http://www.shroomery.org/forums/showflat.php/Number/4141612#Post4141612


--------------------
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Offlinewillmafingerdo
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Re: liquid culture mix [Re: shroomer68]
    #5173182 - 01/12/06 07:31 PM (15 years, 1 month ago)

i use an empty syringe to get the 4 cc of karo and my one mesuring cup has ml on it so i get the 96 cc from that..


--------------------
:scaryshroom: :shitstorm: :mattz: :bongload: :gethigh: :scaryshroom:

I am not lost.. i am going there looking for it.. and when i get there and find it, i will know what it is..

"The way to stop violence is not to go out and let your head be beaten in but to say, you want to take my life risk yours!"

"are you sure u want to eat all them shrooms??"  "yes i want to hear what my mind has to say..."

remember opions are like assholes, everyone has one and they all stink.


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InvisibleLiquidkick
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Registered: 05/03/02
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Re: liquid culture mix [Re: willmafingerdo]
    #5173194 - 01/12/06 07:33 PM (15 years, 1 month ago)

Search: Liquid Culture.


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InvisibleJaeger
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Registered: 10/01/05
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Re: liquid culture mix [Re: Liquidkick]
    #5173213 - 01/12/06 07:36 PM (15 years, 1 month ago)

96cc's of water weighs 96g, so use 4g of karo


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OfflineOatman2000
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Re: liquid culture mix [Re: shroomer68]
    #5173233 - 01/12/06 07:40 PM (15 years, 1 month ago)

if you use empty syringes.... they have cc measurements.

and if your going to measure the karo do it without the needle.


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OfflineHotnuts
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Re: liquid culture mix [Re: Oatman2000]
    #5173250 - 01/12/06 07:44 PM (15 years, 1 month ago)

Or you can use Pure Vanilla extract as well. 2 tablespoons per 500ml's of H20. 3 really hauls ass. Then sterilize in a pc for 25 minutes @ 15psi or steam sterilize for 40 minutes. Easy as pie and Pure Vanilla extract is available at every grocery store on earth. Hauls ass on the colonizing...


Edited by hotnutz (01/12/06 07:47 PM)


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Invisiblejmg5
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Re: liquid culture mix [Re: Hotnuts]
    #5173284 - 01/12/06 07:53 PM (15 years, 1 month ago)

^^
When using vanilla extract how many cc's of spores do you use (for your mix hotnutz)?

thanks.


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OfflineHotnuts
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Re: liquid culture mix [Re: jmg5]
    #5173441 - 01/12/06 08:33 PM (15 years, 1 month ago)

Very little. 1/2 to 1 cc of spore solution..


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Invisiblejmg5
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Re: liquid culture mix [Re: Hotnuts]
    #5173688 - 01/12/06 09:16 PM (15 years, 1 month ago)

I'm assuming when doing this I should use some kind of agitating device, too?


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OfflineHotnuts
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Re: liquid culture mix [Re: jmg5]
    #5173858 - 01/12/06 09:51 PM (15 years, 1 month ago)

No. Just lightly swirl once a day to aerate the contents. 4-6 days your culture will be ready to use for inoculation....


Edited by hotnutz (01/12/06 09:52 PM)


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InvisibleJaeger
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Re: liquid culture mix [Re: Hotnuts]
    #5173894 - 01/12/06 09:57 PM (15 years, 1 month ago)

Pop a marble or 3 in there and that will agitate/break up stuff


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OfflineHotnuts
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Re: liquid culture mix [Re: Jaeger]
    #5174303 - 01/12/06 11:20 PM (15 years, 1 month ago)

I use vented tubes in the lids so I can simply shake the hell out of the jar when it's inoculation time. Or, I use a piece of a stainless steel hose clamp for an agitator with swirling if all of my vented lids are used up....


Edited by hotnutz (01/12/06 11:20 PM)


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Offlinedyzio
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Re: liquid culture mix [Re: Hotnuts]
    #5174327 - 01/12/06 11:26 PM (15 years, 1 month ago)

do you put hydro peroxide in the solution?
how often and how much?
just tell me what you guys use for these amounts


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OfflineHotnuts
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Re: liquid culture mix [Re: dyzio]
    #5174369 - 01/12/06 11:35 PM (15 years, 1 month ago)

Quote:

dyzio said:
do you put hydro peroxide in the solution?
how often and how much?
just tell me what you guys use for these amounts




No peroxide is needed...


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Offlineshroomer68
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Re: liquid culture mix [Re: Hotnuts]
    #5177737 - 01/13/06 06:50 PM (15 years, 1 month ago)

i just finished sterilizing a pint jar of water and honey (1-1.5 tea spoons per 500 ml. will this work? or do i absolutely need to have karo in the mixture??????... if so i might start over again with vanilla extract or i might do both if this one still works.


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Offlineshroomer68
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Re: liquid culture mix [Re: shroomer68]
    #5177809 - 01/13/06 07:06 PM (15 years, 1 month ago)

will this work for that vanilla tek?


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