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 brianjamez
technofool
Registered: 07/12/04
Posts: 149
Loc: Luna
Last seen: 17 years, 5 months
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 Tyvek time! 
#5150204 - 01/07/06 10:18 AM (18 years, 3 months ago) |
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Using a tyvek lid, can you inoculate a jar by peircing the tyvek then covering the hold with something like micropore tape or is the threat of contamination to great? Anybody tried this?
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~plop~
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 agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Tyvek lid? Show me.
If you mean a canning jar ring, with tyvek under it & no metal lid?
That much tyvek area will allow a jar to DRY OUT.
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Sudafeds
Stranger
Registered: 12/17/05
Posts: 15
Last seen: 18 years, 2 months
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Re: Tyvek time! [Re: agar]
#5150232 - 01/07/06 10:30 AM (18 years, 3 months ago) |
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That with a glue gun from walgreens for cheap- is part of my procedure
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brianjamez
technofool
Registered: 07/12/04
Posts: 149
Loc: Luna
Last seen: 17 years, 5 months
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Re: Tyvek time! [Re: agar]
#5150267 - 01/07/06 10:49 AM (18 years, 3 months ago) |
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Two 1/4 inch holes, tyvek under the lid then I take it? How do you inoculate then? Just peirce the tyvek I take it?
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~plop~
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krill
absolute gonzo
Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 9 months
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Re: Tyvek time! [Re: Sudafeds]
#5150269 - 01/07/06 10:49 AM (18 years, 3 months ago) |
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i REALLY like the idea of using Tyvek, but let me get this straight (the most advanced seal i've used is a Ramsey seal)...
JAR + TYVEK SQUARE + LID (W/ INOC. HOLES) + BAND + COFFEE FILTERS + HEAVY RUBBER BAND + FOIL (in my pc anyways) ???
is that right? you don't need polyfill underneath the Tyvek? you just stick the needle thru the Tyvek to inoculate and then tape the hole? what if you don't have a glove box?
is Tyvek the future or just a complicated, fancy seal? like i said, the most advanced seal i've worked with is a Ramsey (foil) seal...
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"DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson
NO LEFT TURN UN-STONED
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Quote:
brianjamez said:
Two 1/4 inch holes, tyvek under the lid then I take it? How do you inoculate then? Just peirce the tyvek I take it?
NOTE the difference in the lids on jars 2 & 3 in the picture above.
Jar lid 2 is a 2 hole lid.
Jar lid 3, is a 3 hole lid.
Either way, inoculate through 1 hole & tape it over.
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krill
absolute gonzo
Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 9 months
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Re: Tyvek time! [Re: agar]
#5150319 - 01/07/06 11:05 AM (18 years, 3 months ago) |
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so you only use one of the holes? (my gf feels the SAME way) what about putting foil under the Tyvek? any comment there?
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"DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson
NO LEFT TURN UN-STONED
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brianjamez
technofool
Registered: 07/12/04
Posts: 149
Loc: Luna
Last seen: 17 years, 5 months
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Re: Tyvek time! [Re: agar]
#5150392 - 01/07/06 11:34 AM (18 years, 3 months ago) |
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Can this be accomplished without a glovebox?
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~plop~
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coda
Banjo Goiter
Registered: 03/20/01
Posts: 8,750
Last seen: 1 year, 26 days
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Re: Tyvek time! [Re: krill]
#5150398 - 01/07/06 11:36 AM (18 years, 3 months ago) |
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foil under the tyvek would defeat the purpose of tyvek. It wouldnt allow for gas exchange. Yes, use only one hole to innoc. I do just one 1/4 inch hole and a small pinhole for innoc. I prefer putting polyfill in the 1/4 inch hole as a preventative measure but its not needed.
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To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .
-JG
i really am glad you came back to us instead of taking the other path. *hug*
-A_S (RIP your final words to me will never be forgotten)
 
Don't fuck with the laughing jesus.
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brianjamez
technofool
Registered: 07/12/04
Posts: 149
Loc: Luna
Last seen: 17 years, 5 months
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Re: Tyvek time! [Re: coda]
#5150411 - 01/07/06 11:43 AM (18 years, 3 months ago) |
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What I guess I don't get is that how can taping over the hole in the lid, after I inoculate, will adequately cover that hole. Is there much of a gap between the tyvek and the lid?
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~plop~
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Rahz
Alive Again
Registered: 11/10/05
Posts: 9,336
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Here's the method I use. Cut out a tyvek square large enough to cover the jar. Place a dab of RTV sealant in the center and pat it down into a coin shape with something wet. Let dry. Drill a single 3/8 inch hole in the center of the jar lid. Place the lid on the jar. Place the tyvek square, with the sealant _down_, over the lid. Screw the rim on. Inject and forget.
Rahz
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rahz
comfort pleasure power love truth awareness peace
"Human beings are born with different capacities. If they are free, they are not equal. And if they are equal, they are not free."
~Aleksandr Solzhenitsyn
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brianjamez
technofool
Registered: 07/12/04
Posts: 149
Loc: Luna
Last seen: 17 years, 5 months
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Re: Tyvek time! [Re: Rahz]
#5150470 - 01/07/06 12:05 PM (18 years, 3 months ago) |
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Interesting -- whats RTV?
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~plop~
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Quote:
brianjamez said:
Interesting -- whats RTV?
R= Rhinogluzen T= Tanglzifin V= Vortofincklr
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JOKE
RTV is a silicone high temp type gasket sealer @ any autoparts store
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Rahz
Alive Again
Registered: 11/10/05
Posts: 9,336
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Gasket sealant. You can get a tube at the auto supply shop for 5 bucks. It will withstand PC temps  and comes in different colors 
Rahz
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rahz
comfort pleasure power love truth awareness peace
"Human beings are born with different capacities. If they are free, they are not equal. And if they are equal, they are not free."
~Aleksandr Solzhenitsyn
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Rahz
Alive Again
Registered: 11/10/05
Posts: 9,336
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Re: Tyvek time! [Re: agar]
#5150510 - 01/07/06 12:17 PM (18 years, 3 months ago) |
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Room Temperature Vulcanizing  didn't know myself.
Rahz
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rahz
comfort pleasure power love truth awareness peace
"Human beings are born with different capacities. If they are free, they are not equal. And if they are equal, they are not free."
~Aleksandr Solzhenitsyn
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TheStudent
Learning
Registered: 05/08/05
Posts: 281
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Re: Tyvek time! [Re: Rahz]
#5150533 - 01/07/06 12:23 PM (18 years, 3 months ago) |
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did someone say coffee filters? can you use them? lol
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agar
old hand
Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: Tyvek time! [Re: Rahz]
#5150538 - 01/07/06 12:25 PM (18 years, 3 months ago) |
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Quote:
Rahz said:
Room Temperature Vulcanizing didn't know myself.
Rahz
Room Temperature Vulcanizing ---->LMFAO........  
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Rahz
Alive Again
Registered: 11/10/05
Posts: 9,336
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What would you want to use those for? 
Rahz
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rahz
comfort pleasure power love truth awareness peace
"Human beings are born with different capacities. If they are free, they are not equal. And if they are equal, they are not free."
~Aleksandr Solzhenitsyn
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krill
absolute gonzo
Registered: 10/25/05
Posts: 521
Loc: mars hotel
Last seen: 11 years, 9 months
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Re: Tyvek time! [Re: Rahz]
#5150603 - 01/07/06 12:44 PM (18 years, 3 months ago) |
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someone was saying he puts 2 coffee filters OVER the jar lid (lid over Tyvek) and secures them w/ a heavy duty rubberband. he said as an added filter/protection for the substrate. he also said you may wanna cover them again w/ foil to prevent any boiling water from getting in.
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"DO NOT ADJUST YOUR MIND. IT IS REALITY THAT IS MALFUNCTIONING." - Robert Anton Wilson
NO LEFT TURN UN-STONED
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Rahz
Alive Again
Registered: 11/10/05
Posts: 9,336
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Re: Tyvek time! [Re: krill]
#5150660 - 01/07/06 12:53 PM (18 years, 3 months ago) |
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coffee filters are for peace of mind. I won't disuade against using them, but if something can get throught the tyvek, it's going through the coffe filter too.
rubber bands are a good idea with half pints, but if you're doing quarts they're not much use.
These opinions are from my limited experience and reading
Rahz
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rahz
comfort pleasure power love truth awareness peace
"Human beings are born with different capacities. If they are free, they are not equal. And if they are equal, they are not free."
~Aleksandr Solzhenitsyn
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