Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery


This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Jar conditions for colonizing
    #514025 -

It's been two weeks since inoculation and nothing has happened inside the jars. Not contamination, not mycelium, nada. Today 4 of the 12 jars were opened up and checked for any internal growth, and there was none. The cakes seemed very dry, so water was added to the surface of two of them, and the other two were stirred and had water added. They were so dry it was hard to break apart the clumps. The other 8 were left alone in case these 4 go green in the next couple days from doing this. Any suggestions for salvaging these jars? Thanks

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: theseeker]
    #514053 -

Start over

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: ]
    #514064 -

Yeah.... so just take the easy way out. Is the dryness the most likely cause? Maybe jars were boiled too much (1 hr). Not sure what happened. Thanks man

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: theseeker]
    #514081 -

I previously boiled my jars for an hour with foil over the top. I never had problems w/ moisture levels as long as my moisture was correct to start with.

Are you using a dry heat source for incubation?

Joshua


--------------------
The Shroomery Bookstore

Great books for inquiring minds!

"Life After Death is Saprophytic!"

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: Joshua]
    #514095 -

Humidity for incubation was approximately 40-60%, just in a sterelite container. Foil was also used over the lids when boiling. Substrate was prepared according to the directions, 3 cups of water for 12 half-pint jars. Not sure what could have happened. Perhaps the conditions were sterile enough, but the spore syringes (from sporechicks) were bunk.

Thanks for the help.

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: theseeker]
    #514165 -

did you wait long enough to allow the jars to cool? if you didn't you killed the spores when you innoculated.

peace, ar393

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: theseeker]
    #514234 -

Also, if you released the steam from your PC before
it completely cooled down, it could have steamed
the moisture right out of your jars.



--------------------
The rest of those, who have gone before us,
cannot settle the unrest of those who follow.
(Finding Forrester)

Extras: Filter Print Post Top
Re: Jar conditions for colonizing [Re: WakingUpLate]
    #514257 -

This is probably what happened. The pot was opened to check to see if there was splashing a few times during the hour. The gasket to the edge of the lid for the PC was warped when bought, so it had to be used as a normal pot.

The jars were allowed to cool overnight - but the directions indicated that during inoculation to work on an open stove set between Warm and 200. It seemed hot after awhile and the purpose of this procedure was not clear (probably another sterilization tech). The heat was turned down, it didn't seem right.

Thanks for help.

Edited by theseeker (01/08/02 10:49 AM)

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   MagicBag.co Certified Organic All-In-One Grow Bags   Left Coast Kratom Buy Kratom Extract

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* Bottom of Jars not colonizing SpaceCruiser 3,670 2 04/25/02 07:39 AM
by frank_grimes
* Jars almost colonized, quick question words 1,725 2 05/28/02 06:28 PM
by XAZIA
* one jar is colonizing faster.. djira19 901 1 05/26/02 07:17 AM
by pleezr
* top of jars not colonizing jtseaweed 1,524 9 02/25/03 04:24 AM
by pillsbury420
* I have a 1 pint jar, 100% colon. But how do I get it out! moorhsum 1,107 7 03/02/03 11:53 AM
by MushMushi
* water floating in the jars during colonization jahfeelirie 1,192 7 10/14/02 07:14 PM
by jahfeelirie
* Mazapatec jar... properly colonized or not? (PIC) LilMerlin 1,197 2 06/03/02 11:49 AM
by DreaMaTrix
* 18 Jars no colonization WHAT GIVES SumGuy 1,709 6 10/23/01 11:35 AM
by SumGuy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,213 topic views. 8 members, 21 guests and 26 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 16 queries.