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Offlineneoboom
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Florida Tek
    #5113718 - 12/28/05 12:08 AM (19 years, 24 days ago)

Ok,

After a couple of years of trials and experience, I thought I'd piece together my current methods, and publish them in whole.

For those naysayers... I know, I know.. this is a combination of many other teks, but with some of my own contributions.

I'm posting this so that others can consider, review, USE, and grow successful variations of this tek for themselves.

neoboom's "Florida Tek"

Required Materials:
1 hammer & nail (to punch inoculation holes in jar lids)
8 Mason Quart jars (purchase by the dozen for around 8 bucks at walmart)
1 pressure cooker (I use Alum. Presto from Wal-Mart, which is perfect for the pressure drain in most all sinks.)
1 sharp chopping knife w/ cutting board or surface
1 large bucket (I use 3 gallon cleaning bucket)
1 pasta type spoon for scooping substrate into jars
8 coffee filters

1 large "Tyvek" mailing envelope (available @ local Post Office)
NOTE regarding tyvek: be careful acquiring these from Post Office.. the U.S. Postal Service has issued memos for Postal Inspectors regarding people using tyvek envelopes for drug related activities. It just happens to be a "Federal Offense" to use these freely available envelopes for anything other than mail... so proceed w/caution.

Ingredients for 8 Quart jars of substrate:

10 cups popcorn (best priced for purchased in bags from walmart)
2 large tomatoes
4 cups vermiculite
1/2 cups calcium carbonate
3 grams Vitamin B-6 (pure stuff powdered in capsule form, I use 6 500mg caps)
3 grams L-Tryptophan (same as B-6 here, powder caps if possible)
1/2 cup corn syrup

Bring popcorn to light boil for 30 minutes, steep for 1 hours, bring to a light boil again for 20 minutes, steep for 12 hours, bring to light boil for 10 minutes, drain....

Drain for 5 minutes with strainer or screen, or...

I pressure drain immediately after the final boil straight from pressure cooker by turning presto cooker upside down in sink (be careful, cooker will be hot!) Do so, after closing with cover now attached, but cap off.. to let water pressure drain while still hot from boil.

You will hear the water drain from steam pressure, and have an audible indication of when it's done.

While draining, take tomatoes, and slice, then dice into small (as tiny as you can get) pieces with sharp knife on cutting surface.

Note: toss the green part (stem area) of tomato, this is actually toxic to people and probably to spores.

Empty drained popcorn into large bucket.

First:
Add 1/2 cup calcium carbonate (adjust ph for things like acidity of tomatoes, etc) and stir gently.

Second:
Add 1/2 cups corn syrup and stir...

Third:
Now gently add diced tomato and then stir in vermiculite, you can probably go less then the 4 cups here, since the stuff is so damn expensive these days. It's only purpose to prevent moisture from accumulating at bottom of jar.

Now fill the jars 2/3Rd's to 90% full (popcorn is easy to shake in quart jars, so whatever you can fully, and evenly distribute between your 8 jars is what you want, just leave at least 10% space for shake room)

Now top jars with custom cut & sized pieces of Tyvek, making sure you have enough over the jar to cover the threads when top is place on.

Put pierced mason lids (center piece) over tyvek, upside down, so punched metal doesn't tear tyvek.

Now place coffee filter over both center lid and tyvek on jar.

Finally, place lid band (outside, screw piece) on top of the rest, and tighten.

Now, pressure cook jars at med-high (about 65%) on stove top, at 15psi, for 70 minutes each.

Let cool.

Then innoc.

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InvisiblePsychoslut
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Re: Florida Tek [Re: neoboom]
    #5113762 - 12/28/05 12:18 AM (19 years, 24 days ago)

What purpose do the tomato's serve?


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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InvisibleDragonaut

Registered: 06/24/04
Posts: 6,487
Re: Florida Tek [Re: Psychoslut]
    #5113823 - 12/28/05 12:34 AM (19 years, 24 days ago)

Really, I was wondering that too. Is it something to do with adding nutes, or adjusting pH, or what?


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:dragon:

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Offlineneoboom
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Re: Florida Tek [Re: Psychoslut]
    #5113854 - 12/28/05 12:42 AM (19 years, 24 days ago)

Tomatoes are a widely available, abundant natural source of the very thing we are using here to get what we want...

The indole alkaloid tryptamine... =)

This direct metabolic path will no doubt increase the potency of your babies; plus the dextrose in the corn syrup.

well... you have the a most potent mix of the "right stuff" to create your magic mushies at full throttle strength.

So this combine with the recent "lemon" ingestion techniques...

Hell, you may need to ingest something like 1/8th of what you used to to get where you want.

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OfflinePsiloman
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Re: Florida Tek [Re: neoboom]
    #5114246 - 12/28/05 04:16 AM (19 years, 24 days ago)

"Tomatoes are a widely available, abundant natural source of the very thing we are using here to get what we want...

The indole alkaloid tryptamine... =)"

That would increase ,as demonstrated by Gartz, psilocin in very good levels but in expense of psilocybin ,am i wrong?

If indeed it is so,then the mushrooms would be perfect for short-term use (you know the majority that eat mushrooms simply -because- they are available) rather than long term use (drying) so one could pick the exact moment for the use not confined by "eat em while they are fresh/available".

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Offlineneoboom
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Re: Florida Tek [Re: Psiloman]
    #5118618 - 12/29/05 11:25 AM (19 years, 23 days ago)
Log in to view attachment

Quoted from Jensens paper:

Psilocybin biosynthesis
The exact nature of the psilocybin biosynthesis pathway (Figure 1) is still controversial.
Tryptamine has been detected in small amounts in psilocybin containing mushrooms (88, 36). 4 hydroxy-tryptophan, fed as a radioactive precursor to Ps. cubensis mycelium culture, was not incorporated into alkaloids, in contrast to tryptophan, tryptamine, N methyl-tryptamine, N,N dimethyl-tryptamine, N,N diethyl-tryptamine, 4 hydroxy-tryptamine, and psilocin (11, 2, 5, 13). These results suggest that the first step in psilocybin biosynthesis is tryptophan decarboxylation to tryptamine. This is in contrast to the mammalian serotonin (5 hydroxy-tryptamine) synthesis from tryptophan, where 5 hydroxylation is the first reaction (13).
Feeding tryptamine to Ps. cubensis yields the highest amounts of psilocybin ever detected in mushrooms (33). In contrast, the feeding of tryptophan had no effect on the psilocybin content of Ps. baeocystis (65) or Ps. cubensis (12), suggesting that tryptophan decarboxylation is the rate limiting or regulated step of the psilocybin biosynthesis pathway.

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Offlineneoboom
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Re: Florida Tek [Re: neoboom]
    #5118630 - 12/29/05 11:28 AM (19 years, 23 days ago)

Not sure if the attachment is coherant, but in short your answer is:

This is perfect for increasing stable psilocybin content for long term use.

IMHO, This is as good as it gets without genetic modification.

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Offlineneoboom
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Re: Florida Tek [Re: neoboom]
    #5118651 - 12/29/05 11:35 AM (19 years, 23 days ago)

You may be asking "why add the Tryptophan if he quotes the paper that suggests it has no effects?"...

The B-6 lifts the lid on the enzymatic rate limiting that occurs, and allows the metabolism to occur at a peak natural ability.

I'd be interested in measurements from anyone with the ability out there to exact what presice increases this provides.

I'd do it, but I don't have access to the lab or materials necessary.

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Invisiblealtarego
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Registered: 10/25/01
Posts: 130
Re: Florida Tek [Re: neoboom]
    #5118997 - 12/29/05 01:42 PM (19 years, 23 days ago)

Do you have any references for the presence of tryptamine in tomatoes? That was news to me.

- AE

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InvisibleFooMan
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Re: Florida Tek [Re: neoboom]
    #5119100 - 12/29/05 02:17 PM (19 years, 23 days ago)

How do you normally fruit using this substrate? As a cake, cased or do you use it as spawn?


--------------------

Quick WBS Prep

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Offlineneoboom
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Re: Florida Tek [Re: FooMan]
    #5120104 - 12/29/05 07:08 PM (19 years, 23 days ago)

I've been searching for reliable measurements of Tryptamine content of tomatoes.. and haven't had much luck..

But I know it's there from documents around the net, and Lycaeum's tryptamin foods matrix.

(apparently Barley and Brie Cheese have it too, don't know how that'd work though)

For casing, I do as follows:

I use a casing of a mixture of 60% Peat , 10% Perlite , %30 verm, with same measurement of B6 -n- Tryptophan listed above (for good measure of potency increase); along with calcium carb ofcourse to buffer.

colonization in pint jars (last run, was quickest yet...)
took just at two weeks from a 2-3 ML spore injection.

Quart Jars took about 2.5 - 3 weeks for full colonization.

Casing, works wonderfully too as expected this week..

growth seen at 1.5 days after casing that indicated it was time for pinning already!

CAM strain is vigorus, and was in refridge storage for 5 months!
Thank you "little guy".

Casing 50% colonized on day 4 (evenly) on surface, with little strands apparent everywhere. Very aggressive growth so far.. wish I had camera.

(may use webcam and laptop this weekend if New Years plans fall through.)

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Offlineneoboom
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Re: Florida Tek [Re: neoboom]
    #5120170 - 12/29/05 07:23 PM (19 years, 23 days ago)

Oh, add (eyeball the amounts) filtered or mineral water to casing mix, before nuking in microwave for 10 minutes.

solid.

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Offlineneoboom
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Re: Florida Tek [Re: neoboom]
    #5120196 - 12/29/05 07:30 PM (19 years, 23 days ago)

Interesting, it seems bananas also have tryptamine.
Saves dicing effort.

hrmmm.

know what I'm gonna try next.

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OfflineNESpores
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Re: Florida Tek [Re: neoboom]
    #5120790 - 12/29/05 10:44 PM (19 years, 22 days ago)

good for you! I am really diggin this tek. I think ideas like this are great, I am gonna nominate this tek for the archive....
In the mean time, lets see some results with bananas and did someone say Barley?

Peace


--------------------
Did your grow fail, and now your out of spores? Problem Solved. Check out my CakeSaver Tek.
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/5236115/an/0/page/0

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Invisiblealtarego
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Registered: 10/25/01
Posts: 130
Re: Florida Tek [Re: NESpores]
    #5121464 - 12/30/05 07:46 AM (19 years, 22 days ago)

I'm sorry but I'm gonna remain skeptical until I see some references.

Are you sure you aren't confusing tyramine and tryptamine? Those are completely unrelated molecules (aside from being amines). A quick google search does show that tyramine is in tomatoes, bananas, all fermented cheeses (brie). Don't know about barley, but it is in beer.

Where is this Lycaeum tryptamine matrix?

I'm not trying to sound critical, but it is important to be able to justify these kind claims with data or references, particularly here in the "Advanced" forum.

- AE

Edited by altarego (12/30/05 07:48 AM)

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Offlinesublimistri
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Re: Florida Tek [Re: altarego]
    #5121569 - 12/30/05 08:54 AM (19 years, 22 days ago)

Whats this got to do with Florida?


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Tradelist
My Ethnobotanical Garden
Cup Greenhouse Tek. Make small sturdy pots (With detachable grenhouse tops) with cups.


Your human friend will have to dose atropine uninformed, I will not spread information that may harm a cat. - Wiccan_Seeker

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Offlineneoboom
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Re: Florida Tek [Re: sublimistri]
    #5121668 - 12/30/05 09:31 AM (19 years, 22 days ago)

Quote:

sublimistri said:
Whats this got to do with Florida?






Here come the bad vibes:Guys, as much as I would love to spend "all" of my time piecing this stuff together to answer all questions for skeptics.. you will have to do a certain amount of your own homework if you aren't convinced of any of the information posted above. I promise you the above information is valid, and well researched.. believe it or dont..
But at least find out for yourself. I'm just sharing my successes with the world here. May have something more scientific in terms of data after the weekend for you guys; right now time's a little tight.

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Invisiblealtarego
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Posts: 130
Re: Florida Tek [Re: neoboom]
    #5123015 - 12/30/05 03:07 PM (19 years, 22 days ago)

Sorry, but asking for references for such claims is standard practice, and nothing to do with promoting 'bad vibes.'

I can assure you that most of us around here have done our fair share of homework over the years, and if we ask for data to back up claims proffered by others it is because we can and have done the same for our own.

Until I hear otherwise, I'm going to assume you made the understandable mistake of confusing tyramine for tryptamine. With all due respect, I suggest others hold off on putting tomatoes, bananas, brie, etc. in their cultures, hoping they will provide added potency, until we hear back from you.

- AE

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InvisibleFooMan
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Re: Florida Tek [Re: altarego]
    #5123143 - 12/30/05 03:29 PM (19 years, 22 days ago)

I'm pretty skeptical too. Looking at  THIS CHART, I don't see anything leading me to believe that tomatoes have much tryptamine in them at all. :shrug:


Bad vibes, coming at ya...... :stonedjerk:


--------------------

Quick WBS Prep

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OfflineCubenisseur
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Re: Florida Tek [Re: FooMan]
    #5123214 - 12/30/05 03:41 PM (19 years, 22 days ago)

The onyt thing that I know of that has Tryptamine in it is turkey. As in the stuff that makes you tired.... Is that a correct notion?

Also, I see that according to the above posted chart that the tomatoes do have Tryptophan, but not Tyramine or Tryptamine!! DOH!

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