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OfflineVisigoth
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Registered: 11/28/01
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mycelium syringes
    #510499 - 01/04/02 11:09 PM (14 years, 10 months ago)

I read that u can cut a piece of mycelium into tiny pieces, put it in some H2O2 and suck it into a syringe and use it to innoculate other jars. Will this speed up the rate of colonization? Which is better: This method or the Honeywater tek? Thanks!!!!

Vis


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OfflineTeRzMaStA
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Registered: 10/08/01
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Re: mycelium syringes [Re: Visigoth]
    #510506 - 01/04/02 11:19 PM (14 years, 10 months ago)

since you are inoculating with live mycellium you do not have to wait for the spores to germinate so this speeds up colonization. read this:

Mycelium Inoculation Technique


Submitted by Anno
This is a significant improvement of the PF-Tek which enables you to grow virtually hundreds of PF-cakes with only one spore syringe! Also the primordia will appear in as little as 16 days after inoculation! The key is first to colonize a sterilized rye jar and then use this mycelium to make a mycelium syringe.

For the first part I basically use the The Foolproof Psilocybe Cubensis Mycelial Culture Technique which can be found at The Shroomery. I use 100g (3,2 ounces) rye + 100 g water + a knife tip of gypsum in a 450 ml (1 pint) jar. There is an important detail to prevent airborne contamination entering the jars (the rye and later the rice-vermiculite!): Take a clean plastic bag and spray the inner side with Lysol. Rub it from outside so the Lysol is evenly dispersed on the inner surface of the bag. When the sterilization time is over, wait a few minutes for the pressure to build down to ambient. Then take a clean, folded cloth or thick gloves, open the cooker and transfer the (burning hot!) jars to the bag. Roll the rest of the bag together, so you get it pretty airtight. As the hot air in the jars is cooling down and its volume decreases, fresh (contaminated!) air from outside is drawn in. This setup will accomplish that the contaminants from the air set down on the wet bag surface and don't come into the jars! The sterilization time should be at least 45 minutes, better 1 hour to assure complete sterilization. You should shake the rye jars immediately after spore inoculation and than once after 3-4 days and after 8 days. The optimum growth temperature is about 29?C (85?F). Now the best part. You will need:

At least one empty plastic syringe, like the one from PF
A small (app. 200 ml) jar with a screw lid (I take one from a spaghetti sauce)
Tin foil
Alcohol lamp
A tea spoon
Punch a hole with a small nail into the middle of the lid. It has to be big enough for the needle of the syringe fits through. Fill the jar with app. twice the amount of water you will need to fill the syringes. (for instance when you have three syringes with each 10 ml to fill, take app. 60 ml).Screw the lid and cover with a double layer of tin foil, sterilize in a pressure cooker and then handle according to the above tips. After it has cooled down to room temperature prepare all for the transfer of the colonized rye to the water filled jar. Wash your hands, clean the working surface, take the colonized rye jar, loosen the lid of the water jar, but don't take it off jet. Sterilize the already washed and Lysol-sprayed spoon over the flame of the alcohol lamp. I use to cool the hot spoon by dipping it in a small glass of alcohol, but you can also wait a few moments the spoon cools down. Than open the rye jar, the water jar and transfer 2 spoon full of colonized rye to the water jar. This should obviously best be done under sterile conditions, but if you work fast an in a draft free room you should accomplish this without contaminating the whole jar. Replace the lids back and screw them tight. The rye jar can be kept in a fridge at least three months and be used over and over again.

Take the water jar, put a finger on the tin foil where the hole is and shake it vigorously at least 3 minutes until you see many tiny mycelium pieces swimming in the water. Take a clean, sterilized syringe, sterilize the tip of the needle over the flame and pull (holding the needle still in the flame) the plunger back 4 millimeters (1/5 inch). This will give you a little airspace so later on you can shake the mycelium water prior to inoculation of the PF jars.

Remove the tin cover, hold the jar at an angle, insert the needle into water and pull the plunger until the syringe is full. Be careful the syringe needle is clean and sterilized or you will contaminate the mycelium water.

That?s it! You can now use this syringe like you would use the spore-water one. You can keep the mycelium syringes in the fridge at least 2 months.

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