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Jalruza
Boot Lover


Registered: 10/09/04
Posts: 1,985
Last seen: 15 years, 8 months
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Sterilization of grains without pressure!
#5100835 - 12/24/05 05:20 AM (18 years, 2 months ago) |
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I found this on overgrow
Fractional sterilization or tyndallization is a method used to destroy bacteria and endospores in preparation of grain spawn(rye, wheat, millet, birdseed...) and agar, which requires no pressure cooker. In this case, the jars fitted with a filter disc or a polyfill lid filter are boiled or steamed at 212?F (100?C) for 30 min in a pot with lid, three days in a row. Between the boiling steps the jars are kept warm, around 30?C(but room temperature will work too), to allow the remaining endospores to germinate. The basic principle behind this method is that any resistant endospores will germinate after the first heating and therefore be susceptible to killing during the second and third heating.
Timetable of the tyndallization (=fractional sterilisation) process
1) Steam heating to 100 ?C for 30 min Vegetative cells are destroyed but endospores survive
2) Incubate at 30?C-37?C overnight Most bacterial endospores germinate
3) Second heat treatment, 100 ?C, 30 min Germinated endospores are killed.
4) Second incubation at 30?C-37 ?C overnight Remaining endospores germinate
5) Third heat treatment, 100 ?C, 60 min Last remaining germinated endospores are killed
-------------------- Time keeps ticking and running away And It's taking us fast to a brand new free dimension Too cool to mention well that's the intention But some of us too dame blind to see Jesus is the King Volume I Jesus is the King Volume II Shroomery MSN club I'm talking to aliens! Volcano Vap and Brain Chakras Hilary Duff!!
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Holydiver
Stranger



Registered: 03/19/01
Posts: 5,156
Loc: The midnight sea
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Re: Sterilization of grains without pressure! [Re: Jalruza]
#5100842 - 12/24/05 05:31 AM (18 years, 2 months ago) |
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That's been around awhile. It's pretty labor intensive and demanding, for something that could otherwise be solved in one hour @ 15 PSI via a pressure cooker.
-------------------- To find a place to live between the negatives and positives.
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HyphalTryptamin
Stranger

Registered: 05/07/05
Posts: 390
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Re: Sterilization of grains without pressure! [Re: Holydiver]
#5100849 - 12/24/05 05:39 AM (18 years, 2 months ago) |
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You could also use Blackout's pioneering microwave method, if one is still hell-bent on avoiding using a pressure cooker : Microwave Growlog
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FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Re: Sterilization of grains without pressure! [Re: Jalruza]
#5100953 - 12/24/05 07:36 AM (18 years, 2 months ago) |
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This method does work, but doing it once will probably convince you to buy a PC!
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Quick WBS Prep
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Roadkill
Retired Shroomery Mod


Registered: 12/11/01
Posts: 22,674
Loc: Montana
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Re: Sterilization of grains without pressure! [Re: Jalruza]
#5101675 - 12/24/05 12:00 PM (18 years, 2 months ago) |
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Anno has been talk about this for years...
fractional sterilization.
It seems like its a royal pain in the ass to me.
If your a broke ass hippie with no $$$...
it might be a good option!~
-------------------- Laterz, Road Who the hell you callin crazy? You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch! Brainiac said: PM the names with on there names, that means they have mushrooms for sale.
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Jalruza
Boot Lover


Registered: 10/09/04
Posts: 1,985
Last seen: 15 years, 8 months
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Re: Sterilization of grains without pressure! [Re: Jalruza]
#5104157 - 12/25/05 04:09 AM (18 years, 2 months ago) |
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I have another silly question, can i sterilise my casing mix like this, 3 times? Or 1 should be enough?
-------------------- Time keeps ticking and running away And It's taking us fast to a brand new free dimension Too cool to mention well that's the intention But some of us too dame blind to see Jesus is the King Volume I Jesus is the King Volume II Shroomery MSN club I'm talking to aliens! Volcano Vap and Brain Chakras Hilary Duff!!
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
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Re: Sterilization of grains without pressure! [Re: Jalruza]
#5104199 - 12/25/05 05:53 AM (18 years, 2 months ago) |
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You want to simply pasteurize casing, don't sterilize. The idea with pasteurization is to allow the bacteria to survive, while killing off the fungi spores such as trichoderma and pennicillium. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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ohmatic
searcher


Registered: 02/28/04
Posts: 6,742
Loc: europe
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Re: Sterilization of grains without pressure! [Re: Roadkill]
#5104229 - 12/25/05 06:47 AM (18 years, 2 months ago) |
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Quote:
Roadkill said: If your a broke ass hippie with no $$$...
it might be a good option!~
hell i re-define broke ass and even i go big gear 
 ghettoclave  peace ohm
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MONOTUB tek HEATBOMB tek RIP #cultivation! ....can't associate? well FUCK U !
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