Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Registered: 09/10/01
Posts: 43
Loc: Troy, NY - USA
Last seen: 21 years, 7 months
Insuring sterility-need advice
    #510024 - 01/04/02 11:07 AM (21 years, 10 months ago)

In Adaptation 25 of the MMGG v3.2, a procedure for insuring the sterility of cakes whose caps are going to be used for spore prints, suggests covering the cake with a glass jar (a tall one so there's enough growing room) and having a clean washcloth placed under it all, so the cake is sealed from the air (and thus any contaminates) in the terrarium but moisture can get through the washcloth for nourishment-I do have a wire mesh on the floor of my ultrasonically humidified terrarium, but my dilemna is, I have had problems with air exchange in the past, and wouldn't this procedure just be one more thing that suffocates the cake? If so, does anyone else have any ideas for accomplishing sterility for this purpose? Oh, and air exchange ideas too. Yeah, that'd be cool. Uh, huh-huh. ;-) Thanks alot guys.

[blue] To see a world in a grain of sand [/blue]
[green] And Heaven in a wildflower- [/green]
[red]To hold infinity in the palm of your hand [/red]
[purple] And eternity in an hour [/purple]

Extras: Filter Print Post Top
Registered: 10/29/00
Posts: 219
Last seen: 21 years, 7 months
Re: Insuring sterility-need advice [Re: MushroomMatt]
    #511647 - 01/05/02 10:34 PM (21 years, 10 months ago)

Polyethylene plastic is permeable to oxygen and possibly to CO2. You could put the cake in one of those stand up type Zip-Loc bags with a little wet perlite in the bottom for moisture. I have done this often and it works. Washcloths are great places for contams to grow so nix on the washcloth.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   North Spore North Spore Mushroom Grow Kits & Cultivation Supplies

Similar ThreadsPosterViewsRepliesLast post
* Sterile Tissue Cloning Procedure BigBlackNinja 2,087 9 03/20/03 09:54 AM
by Anonymous
* Results pasteurized/sterilized dung verticalclone 2,046 6 12/18/01 11:01 PM
by Champion des Champignons
* Sterilize substrate better than pastuerize? megalomando 2,872 7 03/04/04 05:37 PM
by viscid
* Re: Need Advice, storage of agar plates TriGuy 3,143 6 12/19/99 11:58 PM
by Ryche Hawk
* Re: Azure advice anandam 943 1 01/16/00 08:45 PM
by oldmaverick
* Pasteurize or Sterilize (cow dung)? verticalclone 8,187 5 12/10/01 04:17 AM
* No syringe, no sterility, 100% success smoke2mux 2,111 9 12/11/01 06:36 PM
by Jammer
* Please help!!Sterilization???'s Datrutankdogg69 1,377 7 10/04/01 04:57 PM
by Datrutankdogg69

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, Pastywhyte, bodhisatta
598 topic views. 0 members, 3 guests and 2 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2023 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.007 seconds on 14 queries.