Insuring sterility-need advice - Advanced Mycology

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MushroomMatt
newbie

Registered: 09/10/01
Posts: 43
Loc: Troy, NY - USA
Last seen: 21 years, 7 months

Insuring sterility-need advice

#510024 - 01/04/02 11:07 AM (21 years, 10 months ago)

In Adaptation 25 of the MMGG v3.2, a procedure for insuring the sterility of cakes whose caps are going to be used for spore prints, suggests covering the cake with a glass jar (a tall one so there's enough growing room) and having a clean washcloth placed under it all, so the cake is sealed from the air (and thus any contaminates) in the terrarium but moisture can get through the washcloth for nourishment-I do have a wire mesh on the floor of my ultrasonically humidified terrarium, but my dilemna is, I have had problems with air exchange in the past, and wouldn't this procedure just be one more thing that suffocates the cake? If so, does anyone else have any ideas for accomplishing sterility for this purpose? Oh, and air exchange ideas too. Yeah, that'd be cool. Uh, huh-huh. ;-) Thanks alot guys.

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sylo
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Registered: 10/29/00
Posts: 219
Last seen: 21 years, 7 months

Re: Insuring sterility-need advice

[Re: MushroomMatt]
#511647 - 01/05/02 10:34 PM (21 years, 10 months ago)

Polyethylene plastic is permeable to oxygen and possibly to CO2. You could put the cake in one of those stand up type Zip-Loc bags with a little wet perlite in the bottom for moisture. I have done this often and it works. Washcloths are great places for contams to grow so nix on the washcloth.