Home | Community | Message Board


Mycohaus
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop: ½ Pint Jars, Perlite

Jump to first unread post. Pages: 1
OfflineMushroomMatt
newbie
Registered: 09/10/01
Posts: 43
Loc: Troy, NY - USA
Last seen: 15 years, 2 months
Insuring sterility-need advice
    #510024 - 01/04/02 01:07 PM (15 years, 5 months ago)

In Adaptation 25 of the MMGG v3.2, a procedure for insuring the sterility of cakes whose caps are going to be used for spore prints, suggests covering the cake with a glass jar (a tall one so there's enough growing room) and having a clean washcloth placed under it all, so the cake is sealed from the air (and thus any contaminates) in the terrarium but moisture can get through the washcloth for nourishment-I do have a wire mesh on the floor of my ultrasonically humidified terrarium, but my dilemna is, I have had problems with air exchange in the past, and wouldn't this procedure just be one more thing that suffocates the cake? If so, does anyone else have any ideas for accomplishing sterility for this purpose? Oh, and air exchange ideas too. Yeah, that'd be cool. Uh, huh-huh. ;-) Thanks alot guys.


--------------------
[blue] To see a world in a grain of sand [/blue]
[green] And Heaven in a wildflower- [/green]
[red]To hold infinity in the palm of your hand [/red]
[purple] And eternity in an hour [/purple]


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinesylo
addict
Registered: 10/30/00
Posts: 219
Last seen: 15 years, 1 month
Re: Insuring sterility-need advice [Re: MushroomMatt]
    #511647 - 01/06/02 12:34 AM (15 years, 5 months ago)

Polyethylene plastic is permeable to oxygen and possibly to CO2. You could put the cake in one of those stand up type Zip-Loc bags with a little wet perlite in the bottom for moisture. I have done this often and it works. Washcloths are great places for contams to grow so nix on the washcloth.


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop: ½ Pint Jars, Perlite

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

Similar ThreadsPosterViewsRepliesLast post
* Asking advice for fruiting containers, etc psick_psimon 596 3 04/27/05 04:29 AM
by mycofile
* Sterilizing grains with bleach, will subsequent heating trigger dormant endospores. blackout 3,223 10 10/10/09 01:35 PM
by blackout
* Laminar Flow Q - Maintaining Sterility? quart_o_cunt 1,413 8 03/04/02 05:38 PM
by cookiewhore
* casing advice gratefulredhead 845 5 05/13/01 04:30 PM
by gratefulredhead
* Questions about a sterile glove box
( 1 2 all )
themills 3,408 28 08/14/04 02:19 AM
by phucknut68
* Growing without sterile technique PoC 3,122 18 07/15/09 02:13 PM
by Caladan_Shroom
* Sterile Tissue Cloning Procedure BigBlackNinja 1,474 9 03/20/03 11:54 AM
by Anonymous
* looking for advice on inoculation procedure.. mattymonkey 1,187 7 08/03/05 02:03 PM
by Jeremy_Davis

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, bodhisatta
529 topic views. 0 members, 2 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Vaposhop
Please support our sponsors.

Copyright 1997-2017 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.009 seconds on 19 queries.