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InvisibleTsinaglou
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slow pf-albino on quinoa/brf cakes
    #4969795 - 11/22/05 07:26 PM (18 years, 2 months ago)

Prepared pf cakes in the usual way but using 1/2 quinoa flour to see if it increased potency. Injected each one with pf-albino spores at one site (to get as many separate possible albino cakes as possible). That was done on 10-18-05 and none of the jars are more than half colonized. Some are beginning to show denser, whiter mycelium on the growing edge. I've kept them at about 83 degree. I'm afraid they're dehydrating. Should I try injecting water? Or should I cut my losses, cut out the colonized portions and mix them with poo?

Has anybody else had trouble adding quinoa to brown rice flour?


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OfflineESanceOfCyan
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Re: slow pf-albino on quinoa/brf cakes [Re: Tsinaglou]
    #4969827 - 11/22/05 07:31 PM (18 years, 2 months ago)

Do these jars have foil/tape on the lids? If so, Take it off.
Have you flipped your jars upside down to release Co2?
Other than that, if you are sure that they are not contaminated, id suspect water content.
G/L :bongload:


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Invisiblemskip23
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Re: slow pf-albino on quinoa/brf cakes [Re: ESanceOfCyan]
    #4969850 - 11/22/05 07:34 PM (18 years, 2 months ago)

I heard PF doesnt do good on poo


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Offlinebongtoke
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Re: slow pf-albino on quinoa/brf cakes [Re: mskip23]
    #4969880 - 11/22/05 07:42 PM (18 years, 2 months ago)

Hey guys do i have to tip my jars upside down?cause i hadnt once...and their doing fine..but do you guys suggest i do it?


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InvisibleTsinaglou
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Re: slow pf-albino on quinoa/brf cakes [Re: ESanceOfCyan]
    #4969882 - 11/22/05 07:42 PM (18 years, 2 months ago)

Quote:

ESanceOfCyan said:
Do these jars have foil/tape on the lids? If so, Take it off.
Have you flipped your jars upside down to release Co2?
Other than that, if you are sure that they are not contaminated, id suspect water content.
G/L :bongload:




I'll try turning half of them upside down!  Thanks for the idea.  The foil doesn't seem tight enough to cause any real problem.  No visible contaminations in any jar from that batch.  The Pan. cyans done the same day on the same substrate look much better...


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OfflineTrunkspace
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Re: slow pf-albino on quinoa/brf cakes [Re: Tsinaglou]
    #4970101 - 11/22/05 08:28 PM (18 years, 2 months ago)

My friend's PF albinos haven't even germinated yet, and it's been over a month. South Americans started at the same time completed within two weeks, so perhaps it is just a very slow strain?


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InvisibleBlimeyGrimey
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Re: slow pf-albino on quinoa/brf cakes [Re: Tsinaglou]
    #4970966 - 11/22/05 11:19 PM (18 years, 2 months ago)

Quote:

Tsinaglou said:
I'll try turning half of them upside down! Thanks for the idea. The foil doesn't seem tight enough to cause any real problem. No visible contaminations in any jar from that batch. The Pan. cyans done the same day on the same substrate look much better...




its not the fact that the foil is tight, its the fact that co2 is a heavier gas than oxygen, so when you turn them upside down with the foil off it lets the co2 drain out and is replaced by fresh air with more oxygen because of the vaccuum caused by the escaping co2.
just my 2c


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InvisibleTsinaglou
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Re: slow pf-albino on quinoa/brf cakes [Re: BlimeyGrimey]
    #5086675 - 12/20/05 05:46 PM (18 years, 1 month ago)

Turning upside down did the trick, if slowly. Some of them are pinning and so far all are white or blue. I thought there would be a mix of wild types in with the albinos... And they do have a tendancy to grow downwards instead of reaching up...


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InvisibleShnezbit
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Re: slow pf-albino on quinoa/brf cakes [Re: Tsinaglou]
    #5087008 - 12/20/05 07:25 PM (18 years, 1 month ago)

do you mean that you only injected your jars in one spot, not the usuall 4, thinking that less spores on more substrate would give a higher yield?
tsinaglau wrote "Injected each one with pf-albino spores at one site (to get as many separate possible albino cakes as possible)."

if thats what you did than that is why they are taking so long and may not even make it. next time try doing a lc if your low on spores.

sorry if i misunderstood you and am mistakin'. gl.


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InvisibleTsinaglou
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Re: slow pf-albino on quinoa/brf cakes [Re: Shnezbit]
    #5088954 - 12/21/05 09:04 AM (18 years, 1 month ago)

Thanks for your interest, Schnezbit. My concern was that most of the spores wouldn't produce albinos, and so I inoculated more jars, but with fewer spores. The goal being to have a larger chance of isolating an albino even if the wild type was more competitive. So far it looks like they ALL are albino, but only 1/3rd have pinned.

In an LC is there competition and mycelium conversion? I'm wondering if an LC would lower the number of genotypes surviving to colonization? Not important in most cases, but could be when inoculating with a mixed strain (which I thought this was...).

Using only one inoculation site does increase incubation time, but doesn't account for stalling. Turning them upside down was the trick. I'm suspecting using 1/2 quinoa flour wasn't good for growth either.


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InvisibleShnezbit
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Re: slow pf-albino on quinoa/brf cakes [Re: Tsinaglou]
    #5090339 - 12/21/05 03:01 PM (18 years, 1 month ago)

well you got pins so it sounds like colonization worked out for you. congratulations. post some pics of them when they fruit if you can.
gl


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