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Amazon Shop for: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Vermiculite

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InvisibleAzulAgave
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Registered: 10/18/01
Posts: 170
Has any one else had this happen?
    #508353 - 01/03/02 12:08 AM (15 years, 1 month ago)

I just prepared my own syringes and only one jar is kicking ass when its comes to sporulation. When I gave growing a run this time I used PF teks max fruiting formula. I also changed the vermiculite I used. The verm came from a florist in a 20 pound bag. Im a bit pissed at the fact that only four of my jars are doing anything mycelium wise. So far I have no signs of contames. Could I have used too much or too little water in my mix? And is there any sure fire way to know if there is too much or too little water? Im thinking I used too much water because I believe there was already some moisture content in the verm I got from the fucking florist. Has anyone else encountered this? If not I'll just have to nock up my jars with some more spores and sit tight and wait for signs of contames or signs of sporulation..... man this pisses me off.... If anyone else has experienced this let me know so I can be a bit relieved.


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Offlinenobodycares42
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Registered: 11/12/01
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Re: Has any one else had this happen? [Re: AzulAgave]
    #508375 - 01/03/02 12:17 AM (15 years, 1 month ago)

I think I am having this happen right now. :(
I did the jars like 5 days ago.


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OfflineDrAbeLincoln
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Registered: 08/31/01
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Re: Has any one else had this happen? [Re: AzulAgave]
    #508539 - 01/03/02 02:27 AM (15 years, 1 month ago)

Be patient with them...before I built an incubation chamber I had some of my jars take over a week to show any growth


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InvisibleFreefly
mycoman
Registered: 05/30/01
Posts: 40
Re: Has any one else had this happen? [Re: AzulAgave]
    #508628 - 01/03/02 04:29 AM (15 years, 1 month ago)

As long as the mixture was not dripping water it should not be too wet. patience,patience. Could be 5-15 days to show any signs of growth. Keep in dark at 80f.

FF


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InvisibleRoadkill
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Registered: 12/11/01
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Re: Has any one else had this happen? [Re: AzulAgave]
    #508638 - 01/03/02 04:42 AM (15 years, 1 month ago)

Don't put more spores in it.
Use the spores on another Jar.

What temp are you keeping the jars in ?
Try 84 to 86 degrees....they do better.

Loosen the lids so they can breathe.

If the Verm is too moist you will know soon enough.
You will probably see some crap on the bottom.




--------------------
Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleAzulAgave
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Registered: 10/18/01
Posts: 170
Re: Has any one else had this happen? [Re: Roadkill]
    #508810 - 01/03/02 11:49 AM (15 years, 1 month ago)

thanks for the advice. The way the jars are behaving is just odd. The other times I have done this all sorts of shit was growing, contames and mycelium. Now there seems to be nothing. And the jars with mycelium it seems growth has stopped or there is some silent but deadly bacteria infesting everthing. This is a fucked up hobby. To many variables. Getting the temp up will be a bitch not to excited about possibly shelling out for a damn space heater.


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OfflineMickyFinn
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Registered: 12/19/01
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Re: Has any one else had this happen? [Re: AzulAgave]
    #508813 - 01/03/02 11:57 AM (15 years, 1 month ago)

ive got the same problem going with 4 (1) pint jars that i knocked up about 3 weeks ago. i have no growth, and there is moisture build up on the jars, there is no contamination, but i dont have an incubation chamber and my house is about 70. really sux


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InvisibleOlgualion
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Re: Has any one else had this happen? [Re: AzulAgave]
    #508815 - 01/03/02 11:59 AM (15 years, 1 month ago)

"And is there any sure fire way to know if there is too much or too little water?"

You could sacrifice one jar. Lightly sniff out any contams, and break apart the cake in your hand to check for moisture content. Obviously, use one of the jars that is not colonising.


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See the future...


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Offlinenobodycares42
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Re: Has any one else had this happen? [Re: Roadkill]
    #509342 - 01/03/02 09:20 PM (15 years, 1 month ago)

Do they have to be in a temp of 80 to 86??? What is that coldest you can have them so that they will still work?


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OfflineMickyFinn
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Re: Has any one else had this happen? [Re: nobodycares42]
    #509390 - 01/03/02 10:00 PM (15 years, 1 month ago)

from what everyone is saying, it has to be around 85 to start colonizing. 70-72 just seems to be to cold. 3 weeks is to long, even at 70 degrees, at least thats what i think. it must be a real slow strain i found in that headshop...unless its dead.


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Offlinenobodycares42
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Re: Has any one else had this happen? [Re: MickyFinn]
    #509400 - 01/03/02 10:08 PM (15 years, 1 month ago)

What if the temp goes over 85???? like around 90 or 95?


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Offlineupupup
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Re: Has any one else had this happen? [Re: MickyFinn]
    #509405 - 01/03/02 10:14 PM (15 years, 1 month ago)

Ok damn it. First off, keep your jars at 85 degrees(rule of thumb as some strains do better at different temps), second do not crack open your lid to provide air exchange unless you a) first get some good experience and b) have your jars at least 40% colonized. They don't need the exchange until then. You ask for contams if you do that. Why worry about how much water you have unless you didn't follow instructions to the T? 60 cc's is all that each jar requires for the maximum formula. If you had foil on the tops of the jars you should be fine. Get your spores from someone reputable like Ralph or Blahblahblah. You will get colonization at 70 degrees but it will be S-l-o-w. Some myc is slower than others. You don't need to fork out a whole bunch of cash to do this. Just go and get a 10$ fish tank aquarium heater. Read up on a incubation chamber. It's well worth it.


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Support bacteria - they're the only culture some people have.


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Anonymous

Re: Has any one else had this happen? [Re: nobodycares42]
    #509414 - 01/03/02 10:21 PM (15 years, 1 month ago)

Moisture content means alot when working with rice cakes. Too little moisture, the cakes dry out too fast, and if you get any growth at all, it will be mostly overlay. Too much moisture and the mycelium kind of drowns so it won't grow at all. This recipe How to make BRF cakes is very simple and works very well. I recommend using the coarse sized vermiculite, it seems to provide the perfect moisture content. As far as temps are concerned, the lower the temp the slower the growth. 85-86 is real good. Be vigilant, and never let the jars get up to 90 or above, this definitely cooks the mycelium. Peace


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OfflineMickyFinn
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Re: Has any one else had this happen? [Re: upupup]
    #509420 - 01/03/02 10:28 PM (15 years, 1 month ago)

ive all but about given up on those 4 (1) pint jars, but i do understand the colonization process is slow (especially around 70) but i havent quite given up yet, because of the last cc of that headshop syringe is germinating quite nicely in my karo solution.


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InvisibleAzulAgave
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Registered: 10/18/01
Posts: 170
Re: Has any one else had this happen? [Re: ]
    #509621 - 01/04/02 01:34 AM (15 years, 1 month ago)

I followed the PF Tek max fruiting formula to a T. And this is the first time I used my own spore prints. The strain is PF hawiian and this just seems a rediculously long time compared to what it usually takes when I order spores. I mean shit usually I have contames growing in my jars before I get mycelium..... I have nothing! How fucking annoying is that. Right now Im hoping for contames.


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Amazon Shop for: ½ Pint Jars, Aquarium Heater, Brown Rice Flour, Vermiculite

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