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Offlinemycovolution
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Peroxide dunking mycelium
    #5069517 - 12/15/05 11:52 PM (18 years, 5 months ago)

Does anyone know how long it is safe to dunk a myceliated substrate in lightly peroxidated water (roughly 1 tbs to 1 pint of water?)

Sorry if this has already been covered. I did a search and found nothing on peroxidated water dunks.

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OfflineAnnoA
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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5069761 - 12/16/05 01:54 AM (18 years, 5 months ago)

>(roughly 1 tbs to 1 pint of water?)

What concentration does the 1 tbs of peroxide have?

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Offlinemycovolution
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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5069773 - 12/16/05 01:59 AM (18 years, 5 months ago)

3%

and...

the substrate is a hardwood but I don't think that is relevant.

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OfflineAnnoA
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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5069945 - 12/16/05 02:55 AM (18 years, 5 months ago)

1 US pint = 32 US tablespoons so this would be a concentration of 0.1%.
Frankly, I don't how long it would be safe to dunk.
When you are making peroxide agar, you have a concentration of peroxide in agar of ~0.03% which is 3 times lower, on the other hand if you dunk a substrate in water, only a part of the substrate's mass is saturated with water.
So, I would say, it is safe to dunk it in a 0.1% solution.
Ultimately though, you would have to try it out.

Why do you want to dunk it in diluted peroxide?

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Offlinemycovolution
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Re: Peroxide dunking mycelium [Re: Anno]
    #5069999 - 12/16/05 03:19 AM (18 years, 5 months ago)

I had to water the substrate. So I thought I would try this to destroy of contaminant spores when I opened the container. I thought it might be to high so I drained the peroxide water then refilled it with a lower concentration. I think it is still higher than for peroxide agar. I am going put a small amount of peptone in the solution now (1/8 tbl) then drain it in about 2 hours. I will update in a few days. Thank you for your assistance.

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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5070031 - 12/16/05 03:44 AM (18 years, 5 months ago)

Peptone? I assume this could invite contaminats to your substrate.

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Offlinemycovolution
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Re: Peroxide dunking mycelium [Re: Anno]
    #5070103 - 12/16/05 04:38 AM (18 years, 5 months ago)

The first time I soaked the plugs in peroxide water (before I inoculated) I put roughly the same amount of peptone in the liquid before draining. This has pretty much been an experiment in semi sterile dowel culturing. So far I have slow overall mycelial growth but that is to be expected with plugs. There was however an initial burst of growth which may be attributed to the peptone. I would like to see if I get another burst of growth in a few days. Although it may just be the moisture level. When the plugs have mostly colonized I will expand them again without the peptone to see if there is a difference.

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Invisiblearmedia
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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5070215 - 12/16/05 06:24 AM (18 years, 5 months ago)

Why do you think the moisture level is such a problem?

I simmer or soak my dowels until they sink, sterilize, and inoculate with grain spawn, and they colonize just fine in a few weeks. There is more than enough nutrition in the few tbs of grain to give the mycelium a little juice.



Hypholoma sublateritum

If you don't sterilize your wood, I'd suggest coating it lightly with peroxide after soaking (from a spray bottle, at 0.3%), and not adding any supplements.

- A

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Offlinemycovolution
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Re: Peroxide dunking mycelium [Re: armedia]
    #5070247 - 12/16/05 06:42 AM (18 years, 5 months ago)

about 60% of the dowels in the jar had begun to look dry (I noticed this evening.) Now that you mention it I noticed my dowels still float.

I initially let them soak for 3 days but then they sat in the fridge for a week before the spawn was ready and only got a quick dunk (in peroxide and petone water) before inoculating.

Tonight they were floating and they are very lightly colonized right now. Would you suggest soaking in pure water for 24 hrs at refrigerator temps every few days? Or should I just let them be and soak them when they look dry?

Next time I will make sure they are fully saturated before I inoculate.

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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5070501 - 12/16/05 08:38 AM (18 years, 5 months ago)

>Next time I will make sure they are fully saturated before I inoculate.

By soakingh them for a day bringing them to boil.

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Invisiblearmedia
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Re: Peroxide dunking mycelium [Re: Anno]
    #5071855 - 12/16/05 02:42 PM (18 years, 5 months ago)

Just to be clear: the key is to make sure your substrates (whatever they are) are properly moistened before inoculation. Trying to fix a problem after this only invites contamination issues.

Properly moistened usually means fully saturated, but not exploded, overcooked, or sopping. With wood substrates, this is pretty easy to do. Simmer or soak until they sink, and drain very well.

- A

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Re: Peroxide dunking mycelium [Re: armedia]
    #5073572 - 12/16/05 10:12 PM (18 years, 5 months ago)

i have dunked cakes of brf in 25% peroxide solution and they still fruited

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Offlinemycovolution
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Re: Peroxide dunking mycelium [Re: boosthappyvw]
    #5075713 - 12/17/05 04:06 PM (18 years, 5 months ago)

Thats good to know, thank you everyone for sharing.

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InvisibleSillicybin
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Re: Peroxide dunking mycelium [Re: mycovolution]
    #5082045 - 12/19/05 02:05 PM (18 years, 5 months ago)

I assume you mean 1:3 with water from a 3% solution?

25% H2O2 would make QUICK work of a cake. :smile:

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Offlineboosthappyvw
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Re: Peroxide dunking mycelium [Re: Sillicybin]
    #5083147 - 12/19/05 06:34 PM (18 years, 5 months ago)

25% of a 3% solution

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