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OfflineRazman
Stranger

Registered: 07/07/04
Posts: 514
Last seen: 9 years, 3 months
how many spores can you get from a cap?
    #5066680 - 12/15/05 11:05 AM (18 years, 5 months ago)

I'm finally back from 2 weeks of work and my gf has picked all but a few mushrooms.

One is just about ready to collect spores from (its showing a dark color around the edge).

I'm using this Milkman's spore sprint and syringe tek which involves index cards and packing tape.

Once i get all the spores from the cap into a syringe, i plan on diluting it until its nearly clear, so that i can make the most out of what i have.

How many 1/2 pint jars do you think ill be able to inoculate with the spores off 1 mushroom cap? (the size of the cap is a little bigger than a "zero" you make by curling your index finger and thumb)

My goal is 50 jars but i dont think one cap will do it.. remember i want to be as cheap as possible with the spores -- lots of dilluting..

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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: how many spores can you get from a cap? [Re: Razman]
    #5066731 - 12/15/05 11:14 AM (18 years, 5 months ago)

A LC might be your answer :smile:

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OfflineAnnoA
Experimenter
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Folding@home Statistics
Registered: 06/17/99
Posts: 24,166
Loc: my room
Last seen: 1 day, 18 hours
Re: how many spores can you get from a cap? [Re: Razman]
    #5066756 - 12/15/05 11:20 AM (18 years, 5 months ago)

>How many 1/2 pint jars do you think ill be able to inoculate with the spores off
>1 mushroom cap (the size of the cap is a little bigger than a "zero" you make by
>curling your index finger and thumb)


10 syringes => 100 jars

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OfflineRazman
Stranger

Registered: 07/07/04
Posts: 514
Last seen: 9 years, 3 months
Re: how many spores can you get from a cap? [Re: Anno]
    #5066786 - 12/15/05 11:24 AM (18 years, 5 months ago)

wow i just read the honeywater tek.. i think ill try it..

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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: how many spores can you get from a cap? [Re: Razman]
    #5066797 - 12/15/05 11:27 AM (18 years, 5 months ago)

:smile:
Do a search for dry malt extract.  Some people get crazy results with 2% Light Dry Malt Extract, and 2% dextrose.
Good luck!

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Offlinematchbook
Photographer

Registered: 10/02/04
Posts: 854
Loc: Washington
Last seen: 15 years, 8 months
Re: how many spores can you get from a cap? [Re: Anno]
    #5066931 - 12/15/05 11:55 AM (18 years, 5 months ago)

Quote:

Anno said:
>How many 1/2 pint jars do you think ill be able to inoculate with the spores off
>1 mushroom cap (the size of the cap is a little bigger than a "zero" you make by
>curling your index finger and thumb)


10 syringes => 100 jars




I dont think I'd make 10 syringes out of one print. Maybe 5. But not 10.


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Offlineblackout
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Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 13 days
Trusted Cultivator
Re: how many spores can you get from a cap? [Re: matchbook]
    #5066962 - 12/15/05 12:02 PM (18 years, 5 months ago)

some make 100+

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InvisibleAsante
Omnicyclion prophet
Male User Gallery

Registered: 02/06/02
Posts: 87,675
Re: how many spores can you get from a cap? [Re: Razman]
    #5068150 - 12/15/05 05:23 PM (18 years, 5 months ago)

a spore approximately weighs 0.25 nanogram, so given there are lets say 5mg of spores in a print that's 20.000.000 spores, being ten million inoculation spots.

Ten million inoculation points in 50 jars = 200.000 per jar. That's ample!

It really ought to work. given specialized equipment you could do a million jars from one print o_O


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Omnicyclion.org
higher knowledge starts here

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Invisibleagar
old hand
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Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: how many spores can you get from a cap? [Re: Razman]
    #5068278 - 12/15/05 05:55 PM (18 years, 5 months ago)

Index cards SUCK. A moist cap lets lose moist spores. They will stick to an index card. Once stuck to the card, they are hard yo remove.

Glass is GREAT for prints. I am not impressed with tin foil.

I sometimes use plastic laminate. Then, seal the prints with an impuse sealer.



This creates a "spore bag". Simply clip off the corner, inject sterile water, then asperate out the spore solution.

A big thick print, made/laid in the bottom of a wide mouth quart jar - will get you 50 viable syringes. :grin:

Rig a lid like this.


When you remove the mushroom cap, insert a marble in the jar.

Allow the print to dry a day or 2 (polyfil filters will allow that).

Then inject 100 to 500 ml of STERILE water.

(include a couple drops of "jet dry", or "astroglide' into the water before you sterilize it.

Gently swirl the marble around the jar bottom.

That will agitate the spores into solution.

Then, fill syringes - as needed.  :grin:


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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: how many spores can you get from a cap? [Re: agar]
    #5068485 - 12/15/05 06:41 PM (18 years, 5 months ago)

Astroglide eyy :smile:

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InvisiblePsychoslut
The Mother Fucking Bear-o-dactyl
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Registered: 12/10/02
Posts: 20,917
Loc: all up in ya
Re: how many spores can you get from a cap? [Re: Razman]
    #5068548 - 12/15/05 06:54 PM (18 years, 5 months ago)

i remember when i got dvd sized prints from a certain somebody, thos kicked ass.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]

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InvisibleJaeger
Dreamer
Registered: 10/01/05
Posts: 960
Re: how many spores can you get from a cap? [Re: Psychoslut]
    #5068552 - 12/15/05 06:56 PM (18 years, 5 months ago)

lol, I wish I could find someone with an excellent print like that!
(Loose the Avatar :smile:)

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Offlinedr0mni
My Own Messiah
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Registered: 08/21/04
Posts: 2,921
Loc: USF Tampa, Fl
Last seen: 16 years, 11 months
Re: how many spores can you get from a cap? *DELETED* [Re: Jaeger]
    #5068556 - 12/15/05 06:56 PM (18 years, 5 months ago)

Post deleted by dr0mni

Reason for deletion: ...


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: how many spores can you get from a cap? [Re: dr0mni]
    #5068597 - 12/15/05 07:05 PM (18 years, 5 months ago)

How long do you hang onto LC's for? Right now I just stick with spore syringes because I would never use up all the LC. It would become a full time job. The only other advantage I hear is that it colonized quicker because you don't have to wait for germination. But if you do the LC then that's just an extra step anyways.

I'm sure if I had an AA-941 LC I would use LC for sure.

I've got a 3 1/2 inch print I made.... It's like a trophy.


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Btw- You are not what you own!

Edited by musher_420 (12/15/05 07:10 PM)

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