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Offlineupupup
guardian

Registered: 08/25/01
Posts: 889
Loc: George "I love Hitler" Bu...
Last seen: 20 years, 1 month
cloneing: will this work?
    #504716 - 12/30/01 10:07 AM (22 years, 3 months ago)

I don't have a coffee grinder. I was wondering if I took a stem and used a mortar and pestle to grind it up. add the 1/3 cup h2o2 and add sterile water into that to spread it around. Can't do agar right now. Should I go that route or use the core a peice of the stem and use honey water? What's worked best for ya'll?


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Offlinefelixhigh
Scientist
Male User Gallery

Registered: 06/24/01
Posts: 7,565
Loc: Ly
Last seen: 7 days, 3 hours
Re: cloneing: will this work? [Re: upupup]
    #504722 - 12/30/01 10:24 AM (22 years, 3 months ago)

core piece + honeywater + H2O2
then you allow mycelium to grow from the pieces (couple weeks or more, if you're pretty clean). then it should work. and remember... the more you allow mycelium to grow, the less time your jars (bags or whatever) will take to colonize... good luck!

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Invisiblecarbonhoots
old hand

Registered: 09/11/01
Posts: 1,351
Loc: BC Canada
Re: cloneing: will this work? [Re: upupup]
    #505077 - 12/30/01 04:57 PM (22 years, 3 months ago)

mortor/pedstal would'nt be good.


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Anonymous

Re: cloneing: will this work? [Re: carbonhoots]
    #505141 - 12/30/01 06:26 PM (22 years, 3 months ago)

Please explain this tek
if i understand
1take a center piece of stem
2 put in coffee grinder
3 add h202
4 add malt
5 grind
6 fill syringe
7 innoculate
is that it, with no time inbetween

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Anonymous

Re: cloneing: will this work? [Re: ]
    #507086 - 01/01/02 09:21 PM (22 years, 3 months ago)

Well?

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