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 Richard_D_James
enthusiast
Registered: 04/27/01
Posts: 242
Last seen: 16 years, 4 months
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 Help with Poly-fil. 
#504002 - 12/29/01 09:55 AM (23 years, 1 month ago) |
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Sorry for the cross-post, but I haven't recieved a satisfactory answer,
I want to put a poly-fil filter on top of my 1 pint wide mouths. How big of a hole should I drill? How tight should I stuff the poly-fil? Last time I stuffed it as tight as humanly possible and it didn't work out for me. Saying that I need to stuff it "tight" doesn't really narrow it down for me.
Thanks in advance.
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Workman
1999 Spore War Veteran
 
Registered: 03/01/01
Posts: 3,615
Loc: Oregon, USA
Last seen: 3 hours, 24 minutes
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I use a pencil sized hole and I stuff it in as tight as possible. Actually I pull it through until it is a snug as its gonna get. You still need to pressure cook or boil and a covering of foil is a must.
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Richard_D_James
enthusiast
Registered: 04/27/01
Posts: 242
Last seen: 16 years, 4 months
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here was my first attempt:
I used 1/2 pint jars. Made a hole about the size of my pinky (Slightly larger than a pencil) Stuffed as tight as humanley possible with poly fil. After innocing the plates, nothing happened for like 2 weeks so I forgot about them. Went back much later and found some crazy ass comtams on the agar. Like pink, brown, orange molds. It was crazy. Oh btw i did PC these agar plates before use.
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 LuciferX
ape
Registered: 11/18/98
Posts: 731
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i dont think agar really needs air exchange.
Ive found that poly fill does wonders in quarts. but i dont really see the need in agar. ive forgotten about agar plates before and found that they almost always contaminate eventually, i havent had a plate last more than 3 months by itself.
id try again and just use normal lids. save the poly fill for quarts or half pints when they need to breathe
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Edited by LuciferX (12/30/01 03:06 PM)
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Workman
1999 Spore War Veteran
Registered: 03/01/01
Posts: 3,615
Loc: Oregon, USA
Last seen: 3 hours, 24 minutes
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Ahhh, I see. Agar halfpints. Yeah, you probably could have skipped the polyfil for those. But they would stall eventually if the jars were perfectly airtight. Was the polyfil covered with foil? The fact that it took over 2 weeks for something to appear probably means something got through the polyfil. Contaminate spores most likely cannot penetrate the polyfil directly. But if they land on it, they will germinate and press their way through like a tree root through a sidewalk. You must shield the polyfil from falling spores, this goes for filter disks too. Either store in front of a running hepa filter or cap with foil.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
My Instagram
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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 Re: Help with Poly-fil. [Re: Workman]
#505660 - 12/31/01 03:14 AM (23 years, 30 days ago) |
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Is Poly-fil nearly the same texture as cotton? I tried some cotton today in 3/4 in holes on 1/2 pint jars. I pulled the cotton through the lid, covered w/ foil and PC'd. When I went to innoculate I found my needle pushed the cotton right through my hole. It sounds like I made too large of holes and didn't pack the cotton tight enough. Afterwards to correct my blunder I placed non-stick sterile gauze pads over the holes and taped the gauze. I hope the gauze acts as an adequate barrier.
Joshua
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oscill8
*bondage fairy*
Registered: 02/26/01
Posts: 1,215
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 Re: Help with Poly-fil. [Re: Joshua]
#506076 - 12/31/01 05:14 PM (23 years, 29 days ago) |
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polyfil feels like polyester, not cotton- i think cotton may harbor water more easily than poly will... poly doesnt feel like it absorbs water, while cotton very easily wicks it up.
instead of using one hole for polyfil and innoc., you can stuff one hole w/ poly and create another, smaller hole for innoc. only to be taped after innoc. innoc. in a glovebox to reduce the chance of open air contams. just easier and also you dont want to breach your poly barrier yourself (ever had it fall in on you? sucks...) if youre using 1/2pints for pf tek, why not just go verm barrier? or are you using the 1/2's for agar?
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Joshua
Holoman
Registered: 10/27/98
Posts: 5,398
Loc: The Matrix
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Re: Help with Poly-fil. [Re: oscill8]
#506417 - 01/01/02 04:43 AM (23 years, 29 days ago) |
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I did go with a verm layer. I thought a Poly-fil type set-up would encourage good gas exchange.
Joshua
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The Shroomery Bookstore
Great books for inquiring minds!
"Life After Death is Saprophytic!"
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 13 days, 1 hour
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Richard_D_James
enthusiast
Registered: 04/27/01
Posts: 242
Last seen: 16 years, 4 months
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Re: Help with Poly-fil. [Re: Anno]
#509418 - 01/03/02 08:27 PM (23 years, 26 days ago) |
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Thanks a lot anno!
I now see what I was doing wrong & why it didnt work!
When are you going to update your webpage to link to all the "for dummies" lessons?
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Anno
Experimenter
Registered: 06/17/99
Posts: 24,168
Loc: my room
Last seen: 13 days, 1 hour
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>When are you going to update your webpage to link to all
> the "for dummies" lessons?
Now.
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cookiewhore
enthusiast
Registered: 01/03/02
Posts: 385
Last seen: 22 years, 9 months
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Re: Help with Poly-fil. [Re: Joshua]
#546524 - 02/10/02 10:44 AM (22 years, 11 months ago) |
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yer not supposed to inoc through the poly fill hole.
and no, poly fill filter floss is not cotton.
polyester strads, and cotton are different 
if you are doing pf, why dont you have another hole with tape over it for inoculation..
you arent supposed to be able to inoculate throug hthe poly.
hehe
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