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OfflineOwl
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Shiitake - pasteurized substrate * 2
    #5023985 - 12/06/05 04:49 PM (15 years, 1 day ago)

I have been experimenting a bit with growing shiitake on pasteurized sawdust pellets. So far things are going well.

I have started by disinfecting a plastic bucked with lid with boiling water.





I used two of these sawdust bricks. They are 1 kg each


Broke them into smaller pieces and placed them on the bottom of the bucket. I try to keep the time when the lid is of the bucket as short as possible.



Then I carefully measured 2 liters of boiling hot water and pored it over the sawdust pellets.





Close the lid and leave it for a couple of hours to allow the sawdust pellets to break down and finish pasteurization.



After the sawdust has cooled down it is time to inoculate with grain spawn. I use a large amount (2 kg for 4 kg of substrate) of spawn as a supplement to the substrate and to compensate for the less clean practices.



Healthy spawn forms a big lump, holding the grain together.



To inoculate just crumble up the grain spawn, put it on top of the substrate, close the lid and mix the spawn with sawdust by turning and shaking the bucket.

I add the mixture to filter bags, 2 bags of 3 kg in total.





Leave the bags for 40 days to complete spawn run. This is an early fruiting strain that produces fruits before browning.



These bags were inoculated 13 days ago. The shiitake mycelium has covered the whole substrate.



Update 14 Dec:

Yesterday I inoculated some more bags. The collection is slowly growing.






These are the bags that were inoculated last week:



Edited by Owl (12/14/05 07:37 AM)


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Offlineshirley knott
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5024113 - 12/06/05 05:23 PM (15 years, 1 day ago)

fabulous grow log, looking forward to the next post


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Invisibleohmatic
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Re: Shiitake - pasteurized substrate [Re: shirley knott]
    #5024134 - 12/06/05 05:29 PM (15 years, 1 day ago)

i wonder, can u grow shiitake within a tub that u manually fan ?
as i dont have greenhouses or different fruiting chambers anymore
i wonder as id like some tasty mushy :smile:
peace ohm :mushroom2:


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Offlinesocialnorm77
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Re: Shiitake - pasteurized substrate [Re: ohmatic]
    #5024170 - 12/06/05 05:37 PM (15 years, 1 day ago)

do the sawdust bricks have any binders to keep them together? Sometimes those faux logs that are used for fireplaces have some nasty chemicals and such in them. Would hate to run the risk of your shitakes absorbing something that may make you sick.
other than that question, growth looks great, thanks for sharing. -norm

also the species you have fruits before browning over. Does this take about 30 days of the fruit cycle?


Edited by socialnorm77 (12/06/05 05:39 PM)


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Invisiblemicololo2
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Re: Shiitake - pasteurized substrate [Re: socialnorm77]
    #5025902 - 12/06/05 11:21 PM (15 years, 1 day ago)

I can see the date on your bag. The 23 of nov. Can you show us the mushrooms that will come out from these 2 bags?
Thanks


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: socialnorm77]
    #5027102 - 12/07/05 04:12 AM (15 years, 1 day ago)

Yes, you definitely want to avoid the sawdust logs that contain paraffin to hold them together. The label of the logs that I used states that they are 100% natural beech sawdust.

I hope to fruit the first bags in 30 days. This strain can produce healthy fruits appr. 40 days after inoculation. In this period barking is completed.


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Invisiblemicololo2
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5028142 - 12/07/05 01:19 PM (15 years, 17 hours ago)

Looks like to be near the peroxide method?


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Invisiblemah2b1
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5028292 - 12/07/05 02:11 PM (15 years, 16 hours ago)

Quote:

Owl said: After the sawdust has cooled down it is time to inoculate with grain spawn.




So how do you go about sparating the excess water from the sadust?

I am guessing the wood pellets have retained their shape, just are soggy, so you just pour off the excess water and break up the pellet. If not, and the wood pellets break down, do you squeeze off the excess. Please give details of what went on after pasturization and before inoculation?

Thx in advance.

I have access to large quanities of raw hardwood sawdust, mainly oak, and have considered regular pasturization or steam pasturization. I am afraid that since my sawdust is not in pellets, then regular pasturization will leach out all the nutrients, and steam pasturization is pain to the point that I might as well just autoclave.

I have gone over Rush Waynes H2O2 tek, and it will not work with raw sudust, only wood pellets because they are pressuere treated and that destroys the peroxidase enzymes.


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Offlineeris
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5032214 - 12/08/05 02:42 AM (15 years, 4 hours ago)

Very nice work and pics,
good luck.


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: mah2b1]
    #5032480 - 12/08/05 04:25 AM (15 years, 2 hours ago)

There is no excess moisture. I added exactly 2.4 liters of hot water to 2.2 kg (dry weight) of wood pellets. The end substrate will have a moisture content of appr. 52%.

Hot water will cause the logs to break down into sawdust. Giving the bucket a good shake after you have poored in the spawn will break down any lumps that were still compacted.

If this method works than I will also try it with beech chips by just soaking them in hot water and draining them.

I have tried the peroxide tek with these logs but it always resulted in green contams.


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Offlinelardnar
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5059309 - 12/13/05 09:43 PM (14 years, 11 months ago)

Hi Owl,

Where would one find such logs if you don't mind me asking

Thanks


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: lardnar]
    #5061039 - 12/14/05 07:09 AM (14 years, 11 months ago)

I found these ones at the bbq section in the local supermarket. You could try stores and garden shopping centres that sell barbeque equipment and supplies.

According to this guy: http://www.kitchenculturekit.com/Mushroom/sawdust.htm you can buy them in most hardware stores in the US.


Edited by Owl (12/14/05 07:12 AM)


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5197931 - 01/19/06 07:35 AM (14 years, 10 months ago)

The first test bag is kicking-off producing nice fruits. This strain always produces fruits before browning.





I just cut the plastic where the fruitbodies form



Harvested a bit too late:





The first flush is still going. This is what I have harvested so far: (150 gr. The total subtsrate weight is 1 kg)



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Offlinemattymonkey
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5197992 - 01/19/06 08:41 AM (14 years, 10 months ago)

awesome post Owl :thumbup:

definetly giving inspiration.. i am doing an enoki grow right now in jars w/ pasteurized sawdust.. we'll see how that goes..

i think we are really quickly coming to a place of being able to produce mushrooms with less and less resources.. lets keep on this path


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InvisibleDety
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #5198571 - 01/19/06 01:12 PM (14 years, 10 months ago)

Great grow log love all the pictures. :grin:

5 shrooms for you. :thumbup:


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OfflineAnnoA
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #7181906 - 07/16/07 03:01 AM (13 years, 4 months ago)

Any updates on your technique? Have you been pursuing the pasteurized substrate further?


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: Anno]
    #7182208 - 07/16/07 07:38 AM (13 years, 4 months ago)

Yes, nowadays I use the pasteurized sawdust technique for most wood degrading gourmet species. I have tried it successfully with oysters (Pl. ostreatus, Pl. citrinopileatus, Pl. eryngii and H. ulmarius), shiitake, reishi and lion's mane. I had nice results with all of them but the oyster species produce more on pasteurized wheat straw.

I have not changed the technique much. I am no longer using sawdust pellets. Instead I am using a mixture (by dry weight) of 2 parts beech sawdust, 1 part beech wood chips, 3 parts colonized spawn, 1% gypsum and 1% calcium carbonate.

After I added hot water to the sawdust and wood chips, I wrap the bucket in a sleeping bag for 4 hours pasteurization.

So far I lost 3 bags of 25 produced this way to green mold.

It's a good technique for people who do not have access to or space for a large pressure cooker. It allows me to produce 3 kg sawdust blocks with a 9 liter pressure cooker (to produce the spawn).


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Re: Shiitake - pasteurized substrate [Re: Owl]
    #7185568 - 07/17/07 12:05 AM (13 years, 4 months ago)

I met a guy at a conference that used a large milk vessel for tyndalization. He cooked his fortified woodchip bags for eight hours the first day. Allowed them to cool to 80F for awhile and then cooked them again for another eight hours. He spawned them in clean conditions and had 95% success rate with shiitake.

I'm really interested in your experiences with eryngii. Do you think you could supplement wheat straw with sawdust?


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OfflineOwl
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Re: Shiitake - pasteurized substrate [Re: YidakiMan]
    #7186388 - 07/17/07 03:28 AM (13 years, 4 months ago)

Quote:

YidakiMan said:
I'm really interested in your experiences with eryngii. Do you think you could supplement wheat straw with sawdust?




Yes, for sure. You can use wood chips or sawdust (2/3 straw 1/3 wood chips by dry weight) pasteurized in the same container as wheat straw. This mixture gave me the best results. Pure wood based blocks gave a decent first flush but failed to produce additional flushes.

The reason might be that straw logs have a larger moisture reserve to produce these monster mushrooms in additional flushes.


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OfflineFreeSporePrints
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Re: Shiitake - pasteurized substrate [Re: Owl]
    #7187118 - 07/17/07 10:24 AM (13 years, 4 months ago)

Owl learned me his tek and i always use it without contamination!

http://www.freesporeprints.org/smf/index.php?topic=46.0

and here

http://www.freesporeprints.org/smf/index.php?topic=65.0

of the second batch i pasteurized straw with the same tek, just boiling it also 1 hour before and, under Owl and micololo2 suggestion, put the straw into a pillowcase with a eight over.

Fabio :smile:


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Mushrooms, Mycology and Psychedelics >> Gourmet and Medicinal Mushrooms

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