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Offlineshroomzboy
Stranger
Registered: 12/03/05
Posts: 2
Last seen: 18 years, 5 months
neophyte to this bulletin board
    #5009875 - 12/03/05 10:27 AM (18 years, 5 months ago)

i just found your site!! i have been cultivating since 1978 and have had quite a bit success with using saved spore prints, Bob's malt agar, whole rye berries, straw and casing made up of 1/2 peat moss, 1/3 vermiculite nad 1/6 perlite- after all these years i find i occasionally become frustrated if crop failure occurs too often- i ususally put things away and start up again in a few months- this has been a recent downtime for me and i am planning to get back in the saddle after the holidays-

what is all this talk of using poo as a medium?? sterilized straw produces exceptional yields-

i am on my fifth glove box to ensure a sterile field when i transfer spores or mycelium- i have developed a skin allergy to bleach over these many years using it a sterilizing agent in my glove box- is there another product that is as effective??

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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: neophyte to this bulletin board [Re: shroomzboy]
    #5009952 - 12/03/05 10:49 AM (18 years, 5 months ago)

iso alcohol

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OfflineHotnuts
old hand
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Registered: 02/26/05
Posts: 3,436
Loc: Wild Blue Yawnder
Last seen: 1 month, 8 days
Re: neophyte to this bulletin board [Re: backupwards]
    #5010022 - 12/03/05 11:08 AM (18 years, 5 months ago)

Pine Sol that's diluted works well also. Soap and water is fine really....

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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 7 years, 4 months
Trusted Cultivator
Re: neophyte to this bulletin board [Re: shroomzboy]
    #5010084 - 12/03/05 11:26 AM (18 years, 5 months ago)

most of us don't sterilise straw or poo, but pasteurise it for panaeolus species. we sterilise spawn and woodbased fruiting substrates.

that's a long growing career - what're your preferred species?


--------------------
buh

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Offlineshroomzboy
Stranger
Registered: 12/03/05
Posts: 2
Last seen: 18 years, 5 months
Re: neophyte to this bulletin board [Re: shirley knott]
    #5010221 - 12/03/05 12:20 PM (18 years, 5 months ago)

i grow cubensis and have had some success in past- the problem i have found with alcohol is that it is flammable- i use an alcohol lamp in my glove box to sterilize my instruments(loop) between innoculations- when i use lysol i have caused some large flameouts with the alcohol lamp- if i can figure out how to post some pix of my crop i will-

i guess i am old fashioned when i read some of the other methods- i start with a spore print that i make on a sterilized plate- i transfer the spores to agar which i have sterilzed in pint mason jars- (i still use Bob's malt agar from 'mushroompeople')- then i transfer the agar to whole rye grain in which i have powdered a few sticks of chalk in and then cooked for twenty minutes to prepare for digestion by the mycelium- the rye is placed in quart mason jars and sterilized- i use two lids with a hole cut in the middle of each and a coffee filter placed between the lids- then i boil my straw in a 100 quart pot for 8 hours- after cooling overnight in covered pot, i transfer the rye to the straw- i put a layer of straw and alternate with a quart jar of rye berries until i have three layers- let it sit without lights for a few days and then case with a mixture of 1/2 peat moss, 1/3 vermiculite and 1/6 perlite(sterilized of course)- put the flourescents on a 12 hour on/off schedule- hand spray with water twice daily and wait to pick!! i only wish it were as simple as i make it sound- contamination is my biggest problem- sometimes i don't lose any and sometimes i have total crop failure- the sour apple mold isn't so bad, but the black mold causes me night terrors!

thanks for all input on my bleach problem and i am happy to have found other mycophiles like myself!!

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Offlineshirley knott
not my real name
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Registered: 11/11/02
Posts: 9,105
Loc: London Flag
Last seen: 7 years, 4 months
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Re: neophyte to this bulletin board [Re: shroomzboy]
    #5010300 - 12/03/05 12:41 PM (18 years, 5 months ago)







--------------------
buh

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Offlinefalcon
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Registered: 04/01/02
Posts: 8,049
Last seen: 3 hours, 58 minutes
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Re: neophyte to this bulletin board [Re: shroomzboy]
    #5010969 - 12/03/05 04:10 PM (18 years, 5 months ago)

Instead of beach use Quaternay ammonium it's what kills bacteria in a lot of commercial cleaners, like
lysol.

editYou can also get tablets at restraunt and bar supply stores like these or some generic brand. One of these tablets makes about a gallon of disinfectant. They cost like 5 to 10 dollars for a bottle of a 100 tablets.

Listorine also works it contains alcohol,though not as much as Isopropal.
There is thymol in Listorine that helps with the killing of the microbes.


Edited by falcon (12/03/05 04:30 PM)

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Invisibleohmatic
searcher
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Registered: 02/28/04
Posts: 6,742
Loc: europe
Trusted Cultivator
Re: neophyte to this bulletin board [Re: falcon]
    #5011029 - 12/03/05 04:29 PM (18 years, 5 months ago)

mwahahhahahahah great one shirley !
gotta love the freak bros :laugh:
peace ohm :mushroom2:


--------------------
:penis: MONOTUB tek :sun: HEATBOMB tek :penis:

RIP #cultivation! ....can't associate? well FUCK U !

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Invisiblebackupwards
peon

Registered: 04/02/05
Posts: 3,022
Loc: somewhere else
Re: neophyte to this bulletin board [Re: ohmatic]
    #5011288 - 12/03/05 06:07 PM (18 years, 5 months ago)

they must be a european thing, huh?

:peace:

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Offlinezenman223
The last Mohican
Male

Registered: 10/23/05
Posts: 384
Loc: NC
Last seen: 7 years, 5 months
Re: neophyte to this bulletin board [Re: backupwards]
    #5011555 - 12/03/05 07:26 PM (18 years, 5 months ago)

i suggest diluted peroxide it works great, thats what i use for all my sterile teks and it hasnt failed me yet, get a spray bottle, fill half with perox. half water, spray down the room , and then ur glove box, maybe a few sprays in the box while ur working, i think your problem with contanms in the straw is b/c u sterilize it completly and therefor you disrupt the natural balance of bacteria to spore or whatever but it opens it up to contams. u shuold pasterize ur straw and see how that works.


--------------------
"I think I am, therefore I become."
"In the beginning of a change the patriot is a scarce man, and brave, and hated and scorned. When his cause succeeds, the timid join him, for then it costs nothing to be a patriot." - Mark Twain 

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OfflineKaptKid
Spaced Pirate
Male User Gallery

Registered: 12/11/03
Posts: 6,252
Loc: Bright Side of the Sun
Last seen: 4 years, 3 months
Re: neophyte to this bulletin board [Re: shirley knott]
    #5012101 - 12/03/05 10:23 PM (18 years, 5 months ago)

Quote:

shirley knott said:











Love this


:sun:


--------------------
Child of the 60's, Tripping ever since.


:sun:

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Offlinescatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc: Flag
Last seen: 11 years, 4 months
Trusted Cultivator
Re: neophyte to this bulletin board [Re: KaptKid]
    #5016207 - 12/05/05 12:01 AM (18 years, 5 months ago)

Straws alright, I've done that..but then once you try some poo..you dont want to go back..



--------------------
"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay

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Invisiblemicololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
Re: neophyte to this bulletin board [Re: scatmanrav]
    #5016548 - 12/05/05 03:00 AM (18 years, 5 months ago)

Try this, In the holes where you pass your arms in the glove box, install veterinarian insimanation gloves .10$. Where the long gloves starting to be narrow cut them there. Use chirurgical gloves for your hands.10$. You'll still have dexterity and all your arms and hands are cover to protect your self from the stuff you use and your work from you.

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Shop: PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Kraken Kratom Kratom Capsules for Sale   Mushroom-Hut Mono Tub Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Bulk Substrate   Bridgetown Botanicals CBD Concentrates   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck


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