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Offlinesublimistri
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isolating
    #4997530 - 11/30/05 06:43 AM (18 years, 5 months ago)

When you isolate spores using agar , then after you grow some and then clone a mushroom out of the batch , then do a couple more generations , is this isolating a strain , can you name the strain you make?


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InvisibleBlimeyGrimey
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Registered: 08/24/05
Posts: 3,799
Loc: Puget Sound
Re: isolating [Re: sublimistri]
    #4997989 - 11/30/05 10:46 AM (18 years, 5 months ago)

i've always thought it worked this way.

you either isolate dikaryotic mycelium on agar or take a tissue clone from a fruitbody. spawn it to grain or other substrate and fruit it, and then collect its spores, grow those spores and clone the best fruitbody again and repeat the process, do this until a generation predominantly shows the traits you were trying to selectively breed. kinda like how the pf albino grows both normal and albino shrooms, however you are almost guarenteed atleast 1 or 2 albinos per cake/multi-spore inoc. i'd say once you got it to show about 50-60% fruitbodies with the desired selected traits you could call it a strain, but if you were going to try to sell this strain i'd get it to 80 or 90% if not 100% isolated.

i might be wrong on the process, thats just my understanding of it so far.

any experts correct me if i'm wrong.


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Invisiblellamabox
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Registered: 11/23/05
Posts: 564
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Re: isolating [Re: BlimeyGrimey]
    #5003242 - 12/01/05 04:50 PM (18 years, 5 months ago)

Does any body know how many generations of cloning it takes to reach a stable base for a strain? And also how long could this be done before there begins to be a noticeable genetic drift? Would there be any way to breed a specific clone back to its parent mycelium to stabilize the strain?


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