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OfflineRadiant
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Registered: 12/24/04
Posts: 165
Last seen: 16 years, 10 months
PF Jar Tyvek Lids
    #4993222 - 11/29/05 07:27 AM (18 years, 3 months ago)

I have some questions about how to make a lid for a pf jar. I have one pre sterile pf jar remaining and was wondering if I poked 4 holes in the jars lid put a layer of tyvek over the lid and then inoculated if this would be a good sterile way of doing it or should I inoculate and then cover with tyvek. Also some people say wbs has to be pc?ed to be used but Ive heard from others saying you can microwave it and then steam for 90 minutes is there anything to the is tek. Also with the tyvek will this provide enough air exchange in the jar and should I build a glove box to prevent furthere contams because Ive had some major trouble on my first grow only getting one cake colonized. And what would be the best way of making a liquid culture. Should I do the honey tek with a spore syringe or should I clone it using mushroom tissue. Thanks for the help everyone keep it up.

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OfflinePinkeye
Stoner GeniusTrapped in a Labmouses body.
Registered: 11/19/05
Posts: 12
Last seen: 18 years, 2 months
Re: PF Jar Tyvek Lids [Re: Radiant]
    #4994374 - 11/29/05 01:55 PM (18 years, 3 months ago)

Probably need to stick with a PC the microwaving, followed by 90min of steam sound like it'd be drying everything out, maybe not though I've stuck to the BRF so far.  :gethigh:


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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
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Re: PF Jar Tyvek Lids [Re: Radiant]
    #4994428 - 11/29/05 02:09 PM (18 years, 3 months ago)

What you are interested in is Fractional Sterilization , if you want to check into it more  :thumbup:

If you are doing your first LC , I would recommend one of the other TEK's , honey , Karo , or Malt and Dextrose over live tissue transfer . Less chance of contam . Don't have to open your LC to put the tissue in .

Good Luck

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


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Invisiblemusher_420
Stranger

Registered: 08/01/05
Posts: 2,691
Re: PF Jar Tyvek Lids [Re: HippieChick]
    #4994476 - 11/29/05 02:28 PM (18 years, 3 months ago)



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My Humidifier Reservoir Expansion Tek

Must read links for beginners

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OfflineHippieChick
Chicks can do it too!
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Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 2 months
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Re: PF Jar Tyvek Lids [Re: musher_420]
    #4994642 - 11/29/05 03:11 PM (18 years, 3 months ago)

That's a great TEK , but I'm not sure if the sub. is available commercially yet .

Peace,Love,Happiness and Harmony.
:heart: Hippie Chick  :mushroom2:


--------------------
Peace,Love and Happiness
:heart: HC :mushroom2:

Freedoms just another word for nothing left to lose..............

I LUV My Greenhouse
http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848

My First Pans
http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058

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