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StrophariaShaman
Headwind

Registered: 11/13/05
Posts: 26
Loc: Holland
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LC straight to bulk without grain
#4983147 - 11/26/05 01:02 PM (18 years, 5 months ago) |
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I have the patience for spore to agar to isolation to liquid culture to grain to bulk, but it seems quite a few people would like to skip the grain step. It also appears that no one has come up with a very successful tek for transferring LC directly into a bulk substrate. Here is my query. Does anyone believe it would be possible to add LC to a gelatin that was in between the stage of cooling and not yet solidified? If the gelatin was still in liquid form one could add LC and possibly malt or something of that nature, put it in a large tube sprayer and spray the substrate. The gelatin would solidify clinging to all parts of the substrate, the malt would support growth, and the water based gelatin would be thin enough to allow colonization. Definitely needs some experimentation, but does anyone smarter than me see reasons this would be an impossible approach?
*Originally a Cultivation post, re-posted here because of no response.
*Also as a side note, I am curious in the possibilities of nutrient treated gelatin as medium for different stages of growth. If possible, it makes sense to me this would be an incredible contamination preventer. Allowing mass mycelial growth in the core area while the exterior layers of gelatin act almost the same as sandwiching agar.Someone please tell me if this is ludicrous.
-------------------- a wall is just a wall and nothing more at all it can be broken down All posts by this user, whether inquiry or statement, are completely fictitious and for enterainment purposes only. *I am not a shaman, just someone who was really full of himself when he picked his name*
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Dragonaut

Registered: 06/24/04
Posts: 6,219
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I made a thread awhile ago proposing mixing the LC with verm, and using that to spawn to bulk. The verm would act like tiny inoculation points and as you mixed it with the poo, it would distribute LC throughout.
Your idea of spraying it on sounds like it would work well too.
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shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
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Re: LC straight to bulk without grain [Re: Dragonaut]
#4983397 - 11/26/05 02:39 PM (18 years, 5 months ago) |
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you'd have to make sure the verm was saturated, but well dispersed. sounds tricky, wouldn't th verm clump together like burst grains?
-------------------- buh
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StrophariaShaman
Headwind

Registered: 11/13/05
Posts: 26
Loc: Holland
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Re: LC straight to bulk without grain [Re: shirley knott]
#4983451 - 11/26/05 03:02 PM (18 years, 5 months ago) |
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awesome, my thread has been hijacked  buy a mod no less
-------------------- a wall is just a wall and nothing more at all it can be broken down All posts by this user, whether inquiry or statement, are completely fictitious and for enterainment purposes only. *I am not a shaman, just someone who was really full of himself when he picked his name*
Edited by StrophariaShaman (11/26/05 03:45 PM)
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shirley knott
not my real name

Registered: 11/11/02
Posts: 9,105
Loc: London
Last seen: 7 years, 4 months
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sorry. i think the gelatin idea also has merit.
-------------------- buh
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Liquidkick
H2O
Registered: 05/03/02
Posts: 2,635
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Re: LC straight to bulk without grain [Re: shirley knott]
#5000217 - 11/30/05 08:08 PM (18 years, 5 months ago) |
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Blue Helix has been able to sucessfully forgo the grain spawn, check out his grow logs...
Edit: I need to attend Grammar 101.
Edited by Liquidkick (11/30/05 08:13 PM)
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: LC straight to bulk without grain [Re: Liquidkick]
#5016573 - 12/05/05 03:43 AM (18 years, 5 months ago) |
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Skipping grain transfer. I tried it and works well with some strains that grow slow in steril environment. Special receipes need for agar.
LC in mid gelatin-liquid agar. From my opinion it's to risky, the agar still to hot and it's dangerous to damage the culture habilities.
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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 10 months, 5 days
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Re: LC straight to bulk without grain [Re: micololo2]
#5021404 - 12/06/05 12:45 AM (18 years, 5 months ago) |
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"No one" you say? Going from LC to bulk works so well that it's all I do anymore. I get yields that are out of this world doing it. Here are some typical examples of what it's done for me:
     
Those little white ones are Ausi Pan Cyans--blew my head off with those suckers--followed by a picture of some Shiitake. I should have some Goliath Pan Cyans trays fruiting out early Jan. All of these in the pictures were done from LC directly to bulk.
Here is how I prepare the bags:
http://www.shroomery.org/forums/showflat.php?Cat=&Board=faq&Number=4905507
And you CAN go from print to LC directly (did with most of mine), but you have to have a clean print or clean spore syringe. Spores to agar clean up to LC is a safer route. After all, you only need to do agar that one time, and you can store the LC in vacutainers forever after, maybe rejuvinating the culture with a LC-only run anually if you don't use it often. I'd only store a culture in the form of spores if you want to store on the order of decades or want to work on developing a superior substrain later via multi-spore culture clean ups.
If you do go from spore to LC, you need to understand that you can easily make an infected LC if the print is dirty. I was given some infected prints and tried to make LC from them directly awhile back. I learned the hard way what an infected LC looks like and how it acts. What's it look like? Well, I don't know all the flavors, but my little experienced showed me it can look like a super dense LC that is on steriods and develops too fast.
So, what is too fast? A better question is what is the right speed. Given about 3 ml of liquid culture inoculum, I now believe both cubensis and Pan Cyan liquid cultures should take between 3 to 4 days to fully develop even given ideal incubation temperatures. If you inoculated with spores add 2 or 3 days. If you have a stir marble and stir only daily double the figure. If you are using room temperatures (73F) add about 50% additional time.
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mattymonkey
Feel Like aStranger...


Registered: 11/07/04
Posts: 973
Last seen: 11 years, 4 months
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Re: LC straight to bulk without grain [Re: Blue Helix]
#5024747 - 12/06/05 05:33 PM (18 years, 5 months ago) |
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sorry, bluehelix: did i miss that shitake post?
-------------------- "listening for the secret.. searching for the sound.."
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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 10 months, 5 days
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Nah, I didn't post about that one. I do grow a few things, ya know, without making an online log! If you are looking for a new grow logs using LC, you should see a Pan Cyan Goliath log soon after the new year. That's because my Goliath LC finally looks correct like the Ausi one did, but that's after getting off to a few false starts with contaminated spores and a gift contaminated LC growing out to contaminated everything.
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: LC straight to bulk without grain [Re: Blue Helix]
#5025582 - 12/06/05 08:26 PM (18 years, 5 months ago) |
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Excuse me sir I tought it was an advanced mycology forum, but to me now it looks more a magic mushrooms growers forum after revuing the posts. So I'll leave. Wish you good conversations.
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Blue Helix
bold hand


Registered: 02/02/03
Posts: 1,565
Last seen: 10 months, 5 days
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Re: LC straight to bulk without grain [Re: micololo2] 1
#5025859 - 12/06/05 09:12 PM (18 years, 5 months ago) |
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Excuse me, micololo2, the question was asked, and I answered it. It's up to the moderators to police the forum, defintely not you nor me. If you don't like it, you can apply to be moderator and fix it, but don't bitch.
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agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
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Re: LC straight to bulk without grain [Re: micololo2]
#5026413 - 12/06/05 10:55 PM (18 years, 5 months ago) |
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Quote:
micololo2 said: Excuse me sir I tought it was an advanced mycology forum, but to me now it looks more a magic mushrooms growers forum after revuing the posts. So I'll leave. Wish you good conversations.
You think LC to bulk substrate (without colonized grain spawn) isn't advanced? Then, you have something to learn. Doesn't matter if the stain blue, or not.
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: LC straight to bulk without grain [Re: micololo2]
#5027565 - 12/07/05 08:52 AM (18 years, 5 months ago) |
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Quote:
micololo2 said: Excuse me sir I tought it was an advanced mycology forum, but to me now it looks more a magic mushrooms growers forum after revuing the posts. So I'll leave. Wish you good conversations.
Excuse me Blue Helix, but I don't know where you see bitching, It's the last thing I'd do. I just gave a clean opinion. Enjoy sharing and have a good day
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scatmanrav
Brainy Smurf

Registered: 05/08/04
Posts: 11,483
Loc:
Last seen: 11 years, 4 months
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Re: LC straight to bulk without grain [Re: agar]
#5027953 - 12/07/05 10:19 AM (18 years, 5 months ago) |
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Quote:
agar said:
Quote:
micololo2 said: Excuse me sir I tought it was an advanced mycology forum, but to me now it looks more a magic mushrooms growers forum after revuing the posts. So I'll leave. Wish you good conversations.
You think LC to bulk substrate (without colonized grain spawn) isn't advanced? Then, you have something to learn. Doesn't matter if the stain blue, or not.
And to add to that, Shitakke are not magic mushrooms, he posted 3 species, none slow growing, and two happen to be magic (as most of what people grow on this board, with the exception of those on the Edible and Medicinal forum, if magic offends you, perhaps you should stick there, here in this forum you might see magic shrooms and you might see unmagic ones, be prepared....
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: LC straight to bulk without grain [Re: scatmanrav]
#5028081 - 12/07/05 10:55 AM (18 years, 5 months ago) |
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Quote:
scatmanrav said:
Quote:
agar said:
Quote:
micololo2 said: Excuse me sir I tought it was an advanced mycology forum, but to me now it looks more a magic mushrooms growers forum after revuing the posts. So I'll leave. Wish you good conversations.
You think LC to bulk substrate (without colonized grain spawn) isn't advanced? Then, you have something to learn. Doesn't matter if the stain blue, or not.
And to add to that, Shitakke are not magic mushrooms, he posted 3 species, none slow growing, and two happen to be magic (as most of what people grow on this board, with the exception of those on the Edible and Medicinal forum, if magic offends you, perhaps you should stick there, here in this forum you might see magic shrooms and you might see unmagic ones, be prepared....
I don't know where in my text you see me saying LC ... isn't advanced. To prove, I gave my opinion on that subject! Magic doesn't offending me, I start with those. I just gave my opinion: after reviewing the posts... Wow easy to pop you up guys
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mattymonkey
Feel Like aStranger...


Registered: 11/07/04
Posts: 973
Last seen: 11 years, 4 months
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Re: LC straight to bulk without grain [Re: micololo2]
#5028553 - 12/07/05 01:22 PM (18 years, 5 months ago) |
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just wanted to post... he said what he needed with no flaming and kinda got harshed by a few of the experienced folks around here...
micolo has been helping a lot in the gourmet forum and perhaps he came here NOT knowing that it was a magic mushrooms focused site.. we in the gourmet community wish to keep him around..
-------------------- "listening for the secret.. searching for the sound.."
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micololo2
Stranger

Registered: 11/05/05
Posts: 388
Loc: Québec, Canada
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Re: LC straight to bulk without grain [Re: mattymonkey]
#5029018 - 12/07/05 03:09 PM (18 years, 5 months ago) |
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Bull's-eye Mattymonkey, I really didn't know it was a magic mushrooms focus site. Thanks, accept my respects.
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afoaf
CEO DBK?


Registered: 11/08/02
Posts: 32,665
Loc: Ripple's Heart
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Re: LC straight to bulk without grain [Re: micololo2]
#5052016 - 12/12/05 11:50 AM (18 years, 5 months ago) |
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You're fine.
I think that this is just a misunderstanding.
I imagine that micololo2 isn't a native english speaker.
Welcome to the shroomery....stick around!
-------------------- All I know is The Growery is a place where losers who get banned here go.
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Pabu
Stranger
Registered: 03/03/23
Posts: 12
Last seen: 2 hours, 5 minutes
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Re: LC straight to bulk without grain [Re: Blue Helix]
#28657574 - 02/12/24 08:58 PM (3 months, 3 days ago) |
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Hi mate!
The bag prep link seems to be dead, would you mind sharing it again?
Thanks
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