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InvisibleStrophariaShaman
Headwind

Registered: 11/13/05
Posts: 26
Loc: Holland
Isolated dikaryon on agar to liquid culture
    #4981452 - 11/25/05 11:35 PM (16 years, 12 days ago)

I want to germinate on agar so I can isolate one strain (avoiding a multi-spore inoculation) and then colonize a second dish of agar with that strain. Then I want to take a piece(s) of that second agar and start a liquid culture. This seems simple, but I love double checking. Is there any reason I should avoid this? I can always just add colonized grain to liquid and extract, but I would like to try this first.

*Multi-spore is being avoided in order to support yield.


--------------------
a wall is just a wall
and nothing more at all
it can be broken down

All posts by this user, whether inquiry or statement, are completely fictitious and for enterainment purposes only.

*I am not a shaman, just someone who was really full of himself when he picked his name*


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InvisibleHellYeahDude
Shroom Extra

Registered: 11/18/05
Posts: 105
Re: Isolated dikaryon on agar to liquid culture [Re: StrophariaShaman]
    #4981493 - 11/25/05 11:44 PM (16 years, 12 days ago)

You sound like you know your agar shit...can you answer my thread please?


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Offlinelemon_lw
Stranger

Registered: 10/18/04
Posts: 3,622
Loc: That Way
Last seen: 14 years, 2 months
Re: Isolated dikaryon on agar to liquid culture [Re: HellYeahDude]
    #4981540 - 11/25/05 11:55 PM (16 years, 12 days ago)

http://www.shroomery.org/forums/postlist.php/Cat/0/Board/Forum4
youll get better results if you post this here.


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In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz


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