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 absoluteZero
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Registered: 10/27/01
Posts: 111
Last seen: 20 years, 6 months
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 Breeding 
#495909 - 12/19/01 12:28 PM (22 years, 8 months ago) |
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I'm curious how to breed a new "strain". I understand the concepts of isolation on agar, but how can you select traits and is it possible to combine 2 or more strains in to one? Would it be possible to cross breed cubs with something else? Thanks
-zero
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 40oz
Registered: 01/18/01
Posts: 30,119
Loc: Sandy Eggo. Ca.
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its possible but highly unlikely...
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tiny_rabid_birds said:
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absoluteZero
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Registered: 10/27/01
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Re: Breeding [Re: 40oz]
#496163 - 12/19/01 05:15 PM (22 years, 8 months ago) |
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yeah, after i posted, i did a search.... damn thats a lot of work
Hey, thanks for not calling me a retard!
-zero
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kingkc
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Registered: 04/24/00
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Re: Breeding [Re: 40oz]
#496164 - 12/19/01 05:18 PM (22 years, 8 months ago) |
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Interesting that you should ask that question. I've recently seen a cake on flush three with one mushroom in particular that appears to have traits that are totally different than the traits which are traditional for the strain being cultivated.
It is a cubie . the thing started just like all the rest but now that the veil has broken and it has enlarged somewhat I notice that the cap looks sort of like a cool weather variety. What I mean is the appearance of the cap takes on a distinctly carmel cap aura.
I am going to print this an go from there.
I t looks like it could be quite strong .
Is this something that is just a fluke ,you think, and I'm wasting my time going this route?
I was thinking I could get a new substrain going or something llike that.
I've heard stories that usually you can not get any better than nature but people have been doing breeding for a long time and it seems the way to go.
If people would have spent the energy they've spent on pot for the last 40 years we'd have 200 pound watermelons by now.
So I'm printing.
What do you think?
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40oz
Registered: 01/18/01
Posts: 30,119
Loc: Sandy Eggo. Ca.
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...retard! ....;)
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tiny_rabid_birds said:
"your avatar is dirty."
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absoluteZero
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Registered: 10/27/01
Posts: 111
Last seen: 20 years, 6 months
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Re: Breeding [Re: 40oz]
#496200 - 12/19/01 05:45 PM (22 years, 8 months ago) |
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damn smurfs...
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PaleTylenol
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Registered: 11/11/01
Posts: 200
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It's called hybridizing I remember reading an article on it detailing ways to do it,but i forgot to bookmark it so I can't gi ve you the link,but basically what you did was put spores of multiple species into one cake and they would either...
A.Kill eachother in a fight for fungal dominance(almost never occurs)
B.One strain will take over the other(higly likely)
or..
C.They will merge together(so to speak) in mycelluim and fruit a shroom thats 50% one of the species and 50% the other,now this is really rare,but what the hell,go for it,if you get a hybrid you will be so damn proud of ya self...
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[green]"...once the Blanko has reached the stage of being an 'Amp [Meth] head' ... he is a constant menace to his trailer park until he is eliminated."[/green]
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40oz
Registered: 01/18/01
Posts: 30,119
Loc: Sandy Eggo. Ca.
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La Lah lalal lalal La lala La La... (smurf theme)
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tiny_rabid_birds said:
"your avatar is dirty."
Edited by fortyounces2freedom (12/19/01 06:15 PM)
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jonnyshaggs420
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News flash! You cannot cross two seperate species of mushrooms. It is even difficult to do it with individual strains of mushrooms, although not completely impossible. But just sticking them all in one cake and hoping for the best will not likely lend to you getting a hybrid.
Absolutezero-The best route to go for trying to get a flush of mushrooms like the one you described is to clone the odd fruit. If you take a spore print you will not likely see this feature in the next generation. A single spore print of any strain contains all the genetic variations for that particular straom. There are a billion phenotypic characteristics that can be expressed, although most of them are similar there are a few that are different.
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Shdwstr
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Re: Breeding [Re: kingkc]
#496230 - 12/19/01 06:25 PM (22 years, 8 months ago) |
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Clone that puppy... dont print it!
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flaw
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Re: Breeding [Re: Shdwstr]
#496259 - 12/19/01 06:54 PM (22 years, 8 months ago) |
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fortyounces2freedom: that link in your post is broken by the way. :)
I just recently foolishly cased straight wild cubensis from these parts with ecuadors. That was a really dumb idea but never mind. Of course nothing happened, neither strain could get a big enough chunk of itself together to fruit because the cakes were crumbled up and completely scattered.
Maybe if you continiously innoculated cakes with two strains you might have some success but the idea of just putting two strains in and getting results first time is not really going to happen.
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downforpot
Stranger
Registered: 06/25/01
Posts: 5,715
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Re: Breeding [Re: Shdwstr]
#496288 - 12/19/01 07:26 PM (22 years, 8 months ago) |
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how the the fuck was the B+ mushroom made? even though most people consider it weak, it gives me hella nice visuals.
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40oz
Registered: 01/18/01
Posts: 30,119
Loc: Sandy Eggo. Ca.
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ask mothernature...she made it...
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tiny_rabid_birds said:
"your avatar is dirty."
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absoluteZero
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Posts: 111
Last seen: 20 years, 6 months
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Re: Breeding [Re: 40oz]
#496661 - 12/20/01 02:39 AM (22 years, 8 months ago) |
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Just for you 40:
:)
-zero
Edited by absoluteZero (12/20/01 02:43 AM)
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kidrhubarb
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Registered: 09/10/01
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Last seen: 22 years, 5 months
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 Re: Breeding [Re: flaw]
#496782 - 12/20/01 07:50 AM (22 years, 8 months ago) |
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what we are talking about here is the breeding of a basidiomycota, and this means that the two strains have to run into each other, fuse, and then grow out in a dikaryotic state. what i mean by this is that the the two nuclei brought together in mating exist side-by-side in each cell, they only fuse in the basidium. this means that you must get the two separate strains to fuse, before vegetative growth occurs.
kidrhubarb-
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kingkc
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The fruit is still fresh after one day so I'll clone a a portion and print a portion. This characteristic may just be an oddball but I know for a fact that after years and years certain things stay and others go away.Peace.
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40oz
Registered: 01/18/01
Posts: 30,119
Loc: Sandy Eggo. Ca.
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take that monty python foot and shove it up yer ass!!
...lol...j/k....thats awesome man!
..im going to have to use that as my sig pic!
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tiny_rabid_birds said:
"your avatar is dirty."
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fuzzysquirelnuts
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Registered: 06/22/01
Posts: 1,150
Loc: souhwest us
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Re: Breeding [Re: kingkc]
#497205 - 12/20/01 06:22 PM (22 years, 8 months ago) |
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they have 100lb plus watermelons...im not sure if theyve gotten it up to 200lbs yet for watermelon but for pumpkins and some other melon type fruits they have hybradized them to that large of a size
btw-they have spnt quit abit of time hybradizing fruits and vegetables...and they have been doing it for longer than for bud
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were all retarded sometimes
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Anonymous
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Re: Breeding [Re: kingkc]
#497280 - 12/20/01 07:39 PM (22 years, 8 months ago) |
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hybrids can be formed by two distinct Dikaryons of the same species fusing together and exchanging genetic material to form a third distinct dikaryon.
hybrids can also be formed by mating monokaryons from different STRAINS of the same species.
Fruitability is determined by compatible monokaryons, and is not limited to mating within a single STRAIN, but is limited as of now to same species.
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mycotopia
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Registered: 12/20/01
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Last seen: 22 years, 8 months
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This is my first time posting to this site, so I don't know how to start a new thread, but I got a question for a PRO out there about cloning. See the mushroom that looks like a venus flytrap in the pic. If I clone that will all of the mushrooms that I grow from that culture look like that. I understand how "cloning" mushrooms works, but I have never tried it before, they grew from spores. I know that it really technically isn't cloning, but still If I grow from this mushie's tissue, will they all look like this amazing wonder or is it only something I'm going to get to see once in my lifetime?
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srivatsa
Stranger
Registered: 11/13/01
Posts: 53
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Re: Breeding [Re: mycotopia]
#497689 - 12/21/01 08:48 AM (22 years, 8 months ago) |
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If you clone it I would like to see pics of the fruit. You clone to retain the characteristics of the fruit. So they might turn out. You gotta try it to find out.
Edited by srivatsa (12/21/01 08:51 AM)
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MAIA
World-BridgerKartikeya (DftS)
Registered: 04/27/01
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Re: Breeding [Re: mycotopia]
#497709 - 12/21/01 09:34 AM (22 years, 8 months ago) |
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I don't know if it can be achieved, but i also think you have to consider that besides the chances that genetic mutation can be the cause of an external factor, you have to consider the fact that this genetic mutation DNA is not "passed" to the new fruiting body.
Peace,
MAIA
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mycotopia
Stranger
Registered: 12/20/01
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Re: Breeding [Re: MAIA]
#497723 - 12/21/01 10:09 AM (22 years, 8 months ago) |
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Well I'm sure not going to eat this wonderful miracle of life... That leaves me with 2 choices, either I can case it in casting resin, so that it's beauty lives on forever, or I can slice it up and try to clone it... What do you say I should do... I would like to see if it is possible to clone mutations, however I think it would make a nice keychain or lucky charm. you know what I mean? So give me some advice as what to do, I can't make the decision on my own.
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mycotopia
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Registered: 12/20/01
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 Re: Breeding [Re: mycotopia]
#497729 - 12/21/01 10:15 AM (22 years, 8 months ago) |
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which also brings me to another point. If you think it's not possible to clone mutations, then how do you think the PF ALBINO strain came along? it was a mutation of PF Classics, and with no color, so fanaticus cloned it, and luckily the spores carry on the trait most of the time... correct me if I'm wrong, but I believe I am right about how albinos came along.
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mycotopia
Stranger
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Last seen: 22 years, 8 months
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Re: Breeding [Re: mycotopia]
#497735 - 12/21/01 10:26 AM (22 years, 8 months ago) |
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Also one more thing. I have also thought of the fact that it could not be a mutation, but rather something got messed up when it was growing, either I accidently bumped the pin, or maybe it cracked and the cap spliced into 2 parts and grew like that. I don't know which one it is, but If I could clone it and make all of the clones look like that, why boys, I think that we might just have ourselves a new strain of cubensis on our hands!
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gusb232
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Re: Breeding [Re: mycotopia]
#497762 - 12/21/01 11:08 AM (22 years, 8 months ago) |
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That is one cool looking mushy.
I have also seen similar mutionations when i last cased koh samui. and quite a few had these mutaition,( I think i lost the pics when my HD crashed) But one had the muation so pronounced that the cap looked like a wavy edged bowl and it had almost no stem. It didnt get very big because the caseing got run over by green and cobweb mold.
I i have also heard a while back in a post that there is a name for the specific mutation that causes the egdes of caps to turn up a little ( dont know if its the same deal). but this is suposed be to caused by exposure to lysol (i think) or other chemicals. ( sorry , i sound pessemistic)
So it would not be a genetic mutation that could be reproduced, without the same environmental factors.
But in your case is almost looks like the are two mushrooms growing in one.( did you inject two different strains into you subst.?) Did you do anything out of the ordinary to produce this?
try cloning it , see what happends, and let us know.
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Suntzu
Geek
Registered: 10/14/99
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Re: Breeding [Re: gusb232]
#497817 - 12/21/01 12:25 PM (22 years, 8 months ago) |
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That rosecomb thing is not too uncommon, if you really want to induce it to happen, put a thin layer of vaseline on your finger and lightly touch the top of each cap [when young].
The problem with trying to clone the rosecomb effect is that the genetics may not be evenly distributed through the fruit [as in an albino, where you can clone it and the resulting vegetative growth is pigmentally challenged]. Odds are, the mutation/reversion is localized to the area of deformity. If you did try to clone it, be sure to get tissue from the rosecomb itself.
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Suntzu
Geek
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Re: Breeding [Re: mycotopia]
#497823 - 12/21/01 12:34 PM (22 years, 8 months ago) |
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To really get a hybrid going, the procedure would likely be something like this:
1. Obtain spore solutions of the two strains you want to play with, diluted significantly so that a mL or so spread across a petri plate will give ISOLATED germinations [ideally only a handful per plate].
2. Separate and give serial numbers to each and every germination; store in H2O in 96 well plates or similar.
3. Set up "serial crosses" where each well is crossed with a well from the other strain; Perhaps use a microscope to examine the resulting growth, well by well, for the presence of clamp connections or otherwise dikaryotic-looking matches.
4. Attempt to fruit the best candidates; PF style might be the best for large numbers.
This way, you are fairly certain of a hybrid if the spore solution was appropriately diluted and spread. I think some sort of multi-well format is the way to go, but regardless it is a hell of a lot of work. Potentially thousands of 'dud' matings to rule out.
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kingkc
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Re: Breeding [Re: Suntzu]
#497948 - 12/21/01 02:29 PM (22 years, 8 months ago) |
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I f portobellos can happen along then I think if anyone wants to, they can get whatever they think might be a nice variation . Lets face it : selective breeding gets you what you want.
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