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 srivatsa
Stranger
Registered: 11/13/01
Posts: 53
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 Re: Breeding  [Re: mycotopia]
#497689 - 12/21/01 08:48 AM (22 years, 11 months ago) |
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If you clone it I would like to see pics of the fruit. You clone to retain the characteristics of the fruit. So they might turn out. You gotta try it to find out.
Edited by srivatsa (12/21/01 08:51 AM)
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 MAIA
World-BridgerKartikeya (DftS)
 
Registered: 04/27/01
Posts: 7,399
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 3 months, 24 days
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Re: Breeding [Re: mycotopia]
#497709 - 12/21/01 09:34 AM (22 years, 11 months ago) |
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I don't know if it can be achieved, but i also think you have to consider that besides the chances that genetic mutation can be the cause of an external factor, you have to consider the fact that this genetic mutation DNA is not "passed" to the new fruiting body.
Peace,
MAIA
--------------------
Spiritual being, living a human experience ... The Shroomery Mandala
 
Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire
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mycotopia
Stranger
Registered: 12/20/01
Posts: 4
Last seen: 22 years, 11 months
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Re: Breeding [Re: MAIA]
#497723 - 12/21/01 10:09 AM (22 years, 11 months ago) |
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Well I'm sure not going to eat this wonderful miracle of life... That leaves me with 2 choices, either I can case it in casting resin, so that it's beauty lives on forever, or I can slice it up and try to clone it... What do you say I should do... I would like to see if it is possible to clone mutations, however I think it would make a nice keychain or lucky charm. you know what I mean? So give me some advice as what to do, I can't make the decision on my own.
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mycotopia
Stranger
Registered: 12/20/01
Posts: 4
Last seen: 22 years, 11 months
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 Re: Breeding [Re: mycotopia]
#497729 - 12/21/01 10:15 AM (22 years, 11 months ago) |
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which also brings me to another point. If you think it's not possible to clone mutations, then how do you think the PF ALBINO strain came along? it was a mutation of PF Classics, and with no color, so fanaticus cloned it, and luckily the spores carry on the trait most of the time... correct me if I'm wrong, but I believe I am right about how albinos came along.
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mycotopia
Stranger
Registered: 12/20/01
Posts: 4
Last seen: 22 years, 11 months
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Re: Breeding [Re: mycotopia]
#497735 - 12/21/01 10:26 AM (22 years, 11 months ago) |
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Also one more thing. I have also thought of the fact that it could not be a mutation, but rather something got messed up when it was growing, either I accidently bumped the pin, or maybe it cracked and the cap spliced into 2 parts and grew like that. I don't know which one it is, but If I could clone it and make all of the clones look like that, why boys, I think that we might just have ourselves a new strain of cubensis on our hands!
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gusb232
member
Registered: 05/01/01
Posts: 162
Loc: pm me
Last seen: 22 years, 9 months
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Re: Breeding [Re: mycotopia]
#497762 - 12/21/01 11:08 AM (22 years, 11 months ago) |
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That is one cool looking mushy.
I have also seen similar mutionations when i last cased koh samui. and quite a few had these mutaition,( I think i lost the pics when my HD crashed) But one had the muation so pronounced that the cap looked like a wavy edged bowl and it had almost no stem. It didnt get very big because the caseing got run over by green and cobweb mold.
I i have also heard a while back in a post that there is a name for the specific mutation that causes the egdes of caps to turn up a little ( dont know if its the same deal). but this is suposed be to caused by exposure to lysol (i think) or other chemicals. ( sorry , i sound pessemistic)
So it would not be a genetic mutation that could be reproduced, without the same environmental factors.
But in your case is almost looks like the are two mushrooms growing in one.( did you inject two different strains into you subst.?) Did you do anything out of the ordinary to produce this?
try cloning it , see what happends, and let us know.
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Suntzu
Geek
Registered: 10/14/99
Posts: 1,396
Last seen: 4 months, 9 days
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Re: Breeding [Re: gusb232]
#497817 - 12/21/01 12:25 PM (22 years, 11 months ago) |
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That rosecomb thing is not too uncommon, if you really want to induce it to happen, put a thin layer of vaseline on your finger and lightly touch the top of each cap [when young].
The problem with trying to clone the rosecomb effect is that the genetics may not be evenly distributed through the fruit [as in an albino, where you can clone it and the resulting vegetative growth is pigmentally challenged]. Odds are, the mutation/reversion is localized to the area of deformity. If you did try to clone it, be sure to get tissue from the rosecomb itself.
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Suntzu
Geek
Registered: 10/14/99
Posts: 1,396
Last seen: 4 months, 9 days
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Re: Breeding [Re: mycotopia]
#497823 - 12/21/01 12:34 PM (22 years, 11 months ago) |
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To really get a hybrid going, the procedure would likely be something like this:
1. Obtain spore solutions of the two strains you want to play with, diluted significantly so that a mL or so spread across a petri plate will give ISOLATED germinations [ideally only a handful per plate].
2. Separate and give serial numbers to each and every germination; store in H2O in 96 well plates or similar.
3. Set up "serial crosses" where each well is crossed with a well from the other strain; Perhaps use a microscope to examine the resulting growth, well by well, for the presence of clamp connections or otherwise dikaryotic-looking matches.
4. Attempt to fruit the best candidates; PF style might be the best for large numbers.
This way, you are fairly certain of a hybrid if the spore solution was appropriately diluted and spread. I think some sort of multi-well format is the way to go, but regardless it is a hell of a lot of work. Potentially thousands of 'dud' matings to rule out.
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kingkc
enthusiast
Registered: 04/24/00
Posts: 1,064
Last seen: 6 years, 7 months
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Re: Breeding [Re: Suntzu]
#497948 - 12/21/01 02:29 PM (22 years, 11 months ago) |
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I f portobellos can happen along then I think if anyone wants to, they can get whatever they think might be a nice variation . Lets face it : selective breeding gets you what you want.
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