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micololo2
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Registered: 11/05/05
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Boletus edulis (King Boletes)
#4922223 - 11/11/05 01:26 PM (18 years, 2 months ago) |
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I cloned from 2 mushrooms, 2 mycelias. It took 7 days to cover the petri dishes. After I transfered 2 pieces of both new mycelias in new petri dishes and it took only 4 days to cover them again. Does someone know exactly why at the second transfers of the mycelias, it cover the petri dishes faster then when I started from the mushrooms? Does someone know, after grains, which substrat of transfer I can use for inoculation on radicles of the white spruces?
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shirley knott
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4922651 - 11/11/05 03:09 PM (18 years, 2 months ago) |
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you would do well to also post this in the advanced forum
-------------------- buh
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micololo2
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Re: Boletus edulis (King Boletes) [Re: shirley knott]
#4923505 - 11/11/05 07:04 PM (18 years, 2 months ago) |
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I just went there and it looks more magic mushrooms growers than something else
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4923636 - 11/11/05 07:53 PM (18 years, 2 months ago) |
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The mycellium knows how to colonize the medium if you used the same kind of agar on the second dish. It's also been back in the vegetative growing mode for a while.
I don't know what would be best to use on the radicles, the mycellium will grow on a lot of stuff though: Potting soil, soil, coffee grounds, tea, compost...
If I was going to do it I'd use sterilized soil and old white spruce needles.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4923950 - 11/11/05 08:54 PM (18 years, 2 months ago) |
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Falcon, It is something logical to try. What do you think about, taking top soil where the mushrooms grow, sterilize it, and then make the grain transfer. The only thing is, in the transfer substrat will be some grain and could attrack some little animals damaging the inoculation site on the radicles. I'll try to transfer the mycelia from the petri dish right away in the trying substrat. Maybe liquid transfer to? Another thing is trying bolete to colonise hardwood sawdust. This is not logical but black morels mycelia grow well on sawdust and it is not logical. I'll take the PH of the top soil and adjust the trying transfer substrats to this PH. If you have others ideas will be welcome.
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4924448 - 11/11/05 11:04 PM (18 years, 2 months ago) |
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Using soil sounds like a good idea.
The grain will probably attract rodents.
Boletus edulis will grow on sawdust. I don't know how well it will work as an innoculant. I used some B. edulis sawdust to inoculate some 3 year old Black Spruce this spring.
The B. Edulis was found under was some kind of Hemlock. The kind Hemlock is not a native species. I thought it was some kind of spruce tree, because the needles were growing pretty much all the way around the stem. Really dumb mistake.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4925691 - 11/12/05 12:44 PM (18 years, 2 months ago) |
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I didn't find on the market king bolete in vitro culture. Nothing to compare with my mycelia. So less on how to cultivate this species. It will be new experiences for me in the next months. Hope I'll find a way... because I got 72 000, 19 years old white spruces on my lands.
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4926319 - 11/12/05 04:23 PM (18 years, 2 months ago) |
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Penn State's culture list has two different cultures. You are probably better off with the clone that you did yourself.
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mushrx1
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Registered: 04/22/05
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4930924 - 11/13/05 11:42 PM (18 years, 2 months ago) |
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Micololo, that's really good news!
I heard that Boletus was very very slow in culture, but I've never tried to clone it. I have seen Hyphomyces (the parasite) grow fast on agar though. I'm very interested and I hope you are able to fruit it. If you feel it would be better if more people tried to fruit Boletus, I"ll definately give it a try (and I'll trade you whatever you might like that I have growing)
good luck!!
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micololo2
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Re: Boletus edulis (King Boletes) [Re: mushrx1]
#4931461 - 11/14/05 06:48 AM (18 years, 2 months ago) |
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Thank's for your interests and your offer mushrx1, But I have bad news. My culture is infected. I would like to compare my mycelia with a healthy one but who got this picture? The mycelia grow first like the black morel. Thin and fast. After 7 days mycelia get ticker specialy on the bottom walls of the petri dish. At half inch. from the bottom walls white pins form. I now can see little of mold forming on the side wall of the dish. I have'nt seen this before. I'll try an ultimate transfer taking a white part of the mycelia without pins on agar with peroxyde.
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4933863 - 11/14/05 06:54 PM (18 years, 2 months ago) |
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B edulis on agar and birdseed.
These pictures don't show it that well.
The mycellium of B edulis looks very similar to Agaricus bisporus, cottony appressed growth that darkens to tan with age.
It will form primordia on agar. Clamp connections aren't present.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4936404 - 11/15/05 09:16 AM (18 years, 2 months ago) |
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I still have 7 slants of them left. After hours of research on the web, I have ruffly found this. King Bolete have different mycelium characters depend where it comes on earth. Some are slow groing and some are fast. Some have hability to fruit on agar and others not. I did'nt find photos to compare but anough descriptions to know now that my mycelium is a fast growing and have hability to fruit on agar. Mycelia can be transported by insects to start a new colonie!! They are studying methods to grow that mushroom in New Zeeland where I found good informations. I found that to the way you managage the shade in the forest influence the yield of cropping. It's maybe because it needs a lot of trees and lands the reasons why there is so less cultivations of this mushroom in the world? I still motivided to try to grow this mushroom on my lands. I now would like to make a jelly or oil media for an easy fix on radicles of the trees. Do you think it's a good idea? For all the mushrooms I have tried to grow, the black morel is the only one I never have succeed (outside tries).
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4938693 - 11/15/05 07:11 PM (18 years, 2 months ago) |
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Competition from other mycorrhyzal species may be one reason why there are not more people growing B. edulis.
I only find King bolete when the humidity is pretty high. The trees may help to keep the humidity high.
Oil sounds like a great idea. Anything that helps it stick to the radicles. A liquid culture with some oil mixed in and and emulsifier to hold the water and oil together might also work . Oil or jelly would spread on the rootlets much better. Soap if it did not damage the mycellium might be good.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4939376 - 11/15/05 09:42 PM (18 years, 2 months ago) |
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I'll start a culture from a new slant, 6 left. I have stop my choices on vegetable shortning and vegetable oil for my first experiences of making a transfer media for B.edulis. I'll try to use spruces needles to lower the PH. Liquid and agar innoculations will be tried on these substrats. Do you have an idea what I could use to lower the PH? How is going to be the shortning after being sterilized? All questions and experiences make me loving growing mushrooms.
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4940489 - 11/16/05 06:17 AM (18 years, 2 months ago) |
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I don't think it will grow on only oil. Or maybe it will grow very little on just oil. Oil would be good to add after the substrates colonized or mixed with something else. Oil by itself will act as an oxygen barrier. Oil is mostly carbon and hydrogen so there would not be enough nutrition by itself.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4941691 - 11/16/05 01:31 PM (18 years, 2 months ago) |
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Because there is an infection in the petri dish and I'm not use to deal with this one, I'll wait to see how healthy is going to be the mycelium in the slants in several weeks before working on it. In the futur I will take the peroxide agar method for cloning a mushroom. If you still interested to know what's going to happened with this culture please let me know.
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4942764 - 11/16/05 05:55 PM (18 years, 2 months ago) |
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Yes I would like to hear how it goes.
-------------------- Keep growing.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: falcon]
#4944293 - 11/16/05 10:31 PM (18 years, 2 months ago) |
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So later + I'll come back. Thanks for your interst.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#4952349 - 11/18/05 03:46 PM (18 years, 2 months ago) |
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It looks like the plates were infected by the Chaetomium. Rare on plates and probably came from the mushroom. It still white in the slants but my chances to get a good culture are slim.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5138149 - 01/04/06 10:43 AM (18 years, 28 days ago) |
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A good document on Boletus Edulis for who is interested to try to grow them and understand how they grow.
Origine et Qualit? L'exp?rimentation : R?sultats et perspectives
Depuis plusieurs ann?es, la chambre d'Agriculture de la Dordogne m?ne des essais et des observations sur la pousse des c?pes, en collaboration avec l'INRA de Bordeaux et le lyc?e de Vic-en-Bigorre.
En 1995, un r?seau r?gional d'exp?rimentation est mis en place avec deux axes de travail. Biologie Temp?rature Fructification Economie Perspectives
COMPRENDRE LA BIOLOGIE DU C?PE
Quatorze boleti?res r?parties dans les cinq d?partements d'Aquitaine sont ?quip?es d'une station agrom?t?o automatique (r?seau DEMETER).
Trois sont en Dordogne, sur des ?cosyst?mes diff?rents :
ch?nes p?doncul?s
ch?nes rouges
verger de ch?taigniers marigoule.
Les r?sultats cumul?s de plusieurs ann?es d'observations sur une m?me parcelle permettent de formuler des hypoth?ses de plus en plus pr?cises sur l'incidence des param?tres climatiques ? tous les stades du d?veloppement du champignon.
Temp?rature et croissance myc?lienne
Il faut une temp?rature suffisante...
La quantit? de champignons r?colt?s d?pend ?troitement de la quantit? de myc?lium qui s'est pr?alablement d?velopp? dans le sol. En laboratoire, la temp?rature optimale de croissance du myc?lium est comprise entre 20 et 25 ?C. Sur le terrain, les mesures de temp?rature sol r?alis?es entre 5 et 10 cm de profondeur ont permis de calculer des sommes d'heures "chaudes" sup?rieures ? 20 ?C.
Ex: sur quatre pousses comparables, on observe 700 ? 1 000 h o? la temp?rature du sol est sup?rieure ? 20 ?C. Selon les ann?es, selon l'exposition du bois et l'ouverture du peuplement, ces conditions sont plus ou moins atteintes : on ne s'?tonnera pas des diff?rences de rendements d'un bois ? l'autre ou d'une ann?e ? l'autre.
La r?colte de l'ann?e se construit souvent ? partir du myc?lium de l'ann?e pr?c?dente...
L'analyse des r?coltes de Villefranche-du-P?rigord (quantit?s vendues sur le march? en relation avec les donn?es m?t?o de la station de Loub?jac) ou encore chez M. et Mme Chignat laisse supposer que les pousses estivales de c?pes (ao?t-d?but septembre) se d?veloppent principalement ? partir d'un myc?lium de l'ann?e pr?c?dente. En effet, la somme de temp?ratures d'apr?s r?colte est souvent sup?rieure ? la somme de temp?ratures d'avant r?colte.
La fructification
Elle comprend deux phases:
Le d?clenchement de la pousse.
Les observations faites en Dordogne, puis dans de nombreux autres d?partements montrent clairement qu'une pousse de c?pes est toujours d?clench?e par un apport d'eau sur le sol. Cet apport se traduit par une variation de 40 calibars mesur?e sur un tensiom?tre. Cela correspond, en g?n?ral, ? une pluie minimum de 15 mm.
Dans le cas du c?pe d'automne (ou c?pe de Bordeaux (Boletus edulis Bull.), un refroidissement marqu? de la temp?rature est n?cessaire.
D?veloppement des champignons
Le d?lai entre le d?clenchement de la pousse et l'apparition des champignons est de 10 jours environ pour une temp?rature moyenne de 20 ?C (atmosph?re) et jusqu'? 20 jours pour une temp?rature moyenne de 13 ?C. Pendant cette p?riode, la temp?rature de l'air est comprise entre 10 ?C et 25 ?C. Elle peut descendre un peu plus bas pendant quelques heures sans pour autant emp?cher la pousse. Par contre, d?passer 27-28 ?C semble pr?judiciable.
Les conditions d'humidit? sont aussi d?terminantes : le myc?lium qui alimente les futurs champignons en croissance doit trouver de l'eau et de l'oxyg?ne (dans la liti?re et dans les premiers centim?tres du sol). Les valeurs tensiom?triques (mesure de l'humidit? dans le sol) optimales se situent entre 10 et 30 cm. On remarque une diff?rence selon les esp?ces. Le c?pe de Bordeaux (Boletus edulis Bull.) supporte des conditions plus humides que la t?te de n?gre (Boletus aereus Bull.) ou que le c?pe d'?t? (Boletus aestivalis).
L'?tude de ces deux facteurs, humidit? et temp?rature, a permis d'expliquer l'?chec de certaines pousses a priori prometteuses.
LES ?COSYST?MES "C?PES" FAVORABLES
Parall?lement au travail d'observation, une enqu?te de terrain a donn? une somme d'informations ? partir :
de l'exp?rience et des observations des propri?taires, des techniciens forestiers,
de boleti?res ayant des productions importantes et r?guli?res.
Elle permet de dresser un inventaire non limit? des facteurs favorables.
Ouverture du milieu et climat
La densit? du peuplement et sa plus ou moins grande "ouverture" d?terminent l'impact des facteurs climatiques : r?chauffement plus rapide du sol et meilleure p?n?tration des pluies. Cela illustre une phrase devenue courante chez les cueilleurs de champignons "on trouve les c?pes en bordure ou dans les clairi?res ".A l'inverse, un bois trop ouvert peut g?ner la fructification en p?riode de fortes chaleurs.
Le sol
Le syst?me racinaire est d'autant plus mycorhiz? que le sol est pauvre (acide, pauvre en phosphore, potassium...). Un sol a?r? o? r?gne une bonne activit? biologique est un bon indicateur : la liti?re de feuilles ou d'aiguilles d?grad?e alimente le myc?lium.
Gare au tassement !
La composition du peuplement
De nombreuses essences sont associ?es au c?pe : le ch?ne p?doncul?, le ch?ne d'Am?rique, le ch?ne rouvre, le h?tre, le ch?taignier, le charme, le bouleau.., chez les feuillus. L'?pic?a, le sapin de Vancouver, le pin sylvestre... chez les r?sineux. Le m?lange des essences, une essence dominante avec une essence de sous-?tage para?t ?tre tr?s favorable.
LES PERSPECTIVES
La prochaine ?tape, en dehors des travaux d'observation qui se poursuivent, est de tester des techniques sur :
l'am?lioration des boleti?res existantes : nettoyage, ?claircie, d?tourage,
des nouveaux peuplements (pr?c?dent favorable) : essences, densit?, v?g?tation associee.
Dans cet objectif :
Une action c?pe (am?nagement de boleti?re) est propos?e dans le projet CTE collectif for?t. Deux journ?es au mois d'octobre 2001 seront organis?es avec les techniciens forestiers F. Dercq et Patrick Rey. Par la visite de diff?rents peuplements, il s'agira de voir comment gestion sylvicole et production de c?pes sont compatibles.
Nathalie Seegers, Chambre d'Agriculture de la Dordogne pour en savoir plus vous trouverez un dossier au pages 16-17 du P?rigord Hebdo du 24/08/2001 --------------------------------------------------------------------------------
Edited by micololo2 (01/04/06 10:45 AM)
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Speeker

Registered: 02/11/04
Posts: 877
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5139209 - 01/04/06 02:58 PM (18 years, 28 days ago) |
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falcon


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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5140641 - 01/04/06 08:33 PM (18 years, 27 days ago) |
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In the document Google translated essence as gasoline this does not seem right. Any idea what it should read? From context it seem to be essence = essence in English. thanks
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Anno
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Re: Boletus edulis (King Boletes) [Re: falcon]
#5142110 - 01/05/06 02:18 AM (18 years, 27 days ago) |
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Species of tree.
"De nombreuses essences sont associ?es au c?pe "
"A number of tree species are associated with the Boletus."
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falcon


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Re: Boletus edulis (King Boletes) [Re: Anno]
#5143761 - 01/05/06 04:01 PM (18 years, 27 days ago) |
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Thanks Anno it was a bit confusing.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5901119 - 07/26/06 05:28 AM (17 years, 6 months ago) |
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Pounds of boletus edulis harvested from my 30ftX50ft experimentation square of boletus edulis cultivation (in a white spruces plantation). Only a few were found on the other places in the spruces plantation.

Price of the porcini (where I sale my muhrooms) at the farmer market. LB mean pound in english.

Other prices. Pleurotte mean Oyster

White Elm bouquet. I have good prices for them.
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eris
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5901290 - 07/26/06 07:42 AM (17 years, 6 months ago) |
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Wow, I love king boletes. 
Great pics!
-------------------- Immortal / Temporarily Retired The OG Thread Killer My mushroom hunting gallery
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Anno
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5904692 - 07/27/06 09:17 AM (17 years, 6 months ago) |
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Can you outline your technique?
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Cryogenicz
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5904774 - 07/27/06 09:52 AM (17 years, 6 months ago) |
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hey, whats is your first language?
-------------------- www.MycoPath.com Mushroom Spawn, Cultures, Fungi Bags, Casings, Master Grain Jars, Bags for In-vitro, Laboratory supplies, and much more! Mushroom Supplies. Fast Turnaround Times. Great Service. orders@mycopath.com enter code shroomery for 10% off product. www.FungiForum.com
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falcon


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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5906220 - 07/27/06 06:10 PM (17 years, 6 months ago) |
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What did you use to inoculate the roots?
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Speeker

Registered: 02/11/04
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5907137 - 07/27/06 10:15 PM (17 years, 6 months ago) |
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hmm.. do you water that area?
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5909080 - 07/28/06 02:24 PM (17 years, 6 months ago) |
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My technic is not scientificly proved. I want to work more on it before talking about it. I don't want to sound stupid. I still looking for a good agar media and transfer substrat for this species. Any help would be appreciated.
My first language is french.
I don't water the area.
Thank's for your interest and support
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Feelers
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5909936 - 07/28/06 08:11 PM (17 years, 6 months ago) |
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Awesome stuff mico!!! 
Did you just spread a massive patch of a promising isolate outside? Did you try more than one isolate? What did you look for in selection?
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Feelers]
#5921315 - 08/01/06 09:28 AM (17 years, 5 months ago) |
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This sound ridiculous. I took the bottom parts and old caps (not to old)of Boletus and crushed them on rootlets of 20 years old white spruces. I have to find the rootlets in the ground (this is quite a job), then crush the parts of the mush. and cover them exactly how it was. After, I wet them with 1 gallon of water per square feet. I'm not sure if it works, but I get a lot more Boletus where I do this then anywhere else in the tree plantation. I now want to try this in the Black Spruces plantation. I'd like to make a transfer substrate that I could work with, but all my tries to clone this mush. failed in big contam.
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Speeker

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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5921685 - 08/01/06 12:08 PM (17 years, 5 months ago) |
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no it doesn't sound ridiculous.. you got mushrooms, eh? 
maybe it was just the irritation that did the trick or maybe you have found something there.. 
Others have succeed with some "strange" methods before.. http://www.shroomery.org/forums/showflat.php/Number/2685865
and i remember one guy at http://groups.yahoo.com/group/La-truffe that used that kind of natural method with the truffle..
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Feelers
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5923375 - 08/01/06 09:52 PM (17 years, 5 months ago) |
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Quote:
This sound ridiculous.
Well perhaps... , however you seem to be the guy with all the boletus!!
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Owl
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5924124 - 08/02/06 01:08 AM (17 years, 5 months ago) |
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Not at all ridiculous. In Stamet's book "Mycelium Running" he also mentions a few anecdotes of people who had success growing edible mycorrhizal mushrooms by adding stem cuttings to the soil around the tree roots and watering trees with water that was used to wash the mushrooms.
It also has nice pictures of a mushroom (i do not remember which species but I believe it was also mycorrhizal) disintegrating, forming a mycelial network which is quickly incorporated in the forest soil.
Great book..
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micololo2
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Loc: Québec, Canada
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Re: Boletus edulis (King Boletes) [Re: Owl]
#5924468 - 08/02/06 05:35 AM (17 years, 5 months ago) |
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I'll try to do the same with chanterelle on Fir rootlets, I'll see next year...
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Feelers
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5925563 - 08/02/06 02:14 PM (17 years, 5 months ago) |
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Nice work and pics Mico - your work/success with gourmets is always very impressive!
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Speeker

Registered: 02/11/04
Posts: 877
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Re: Boletus edulis (King Boletes) [Re: Anno]
#5927266 - 08/02/06 10:51 PM (17 years, 5 months ago) |
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gasoline is still gasoline, but article looks much nicer without those question marks..
http://translate.google.com/translate?u=...Flanguage_tools
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5927835 - 08/03/06 05:02 AM (17 years, 5 months ago) |
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"Essence" could mean gasoline or a specie of a tree.
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Speeker

Registered: 02/11/04
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5927988 - 08/03/06 08:00 AM (17 years, 5 months ago) |
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i know.. 

Some Boletus sp. growing from a bottle .. weird looking, eh?
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5928160 - 08/03/06 10:05 AM (17 years, 5 months ago) |
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Amazing!!! What kind of Boletus is this one and how did you do that? I do not understand, Boletus are supposed to be mycorrhiza mushrooms, how can they pop up without a tree in an artificial environment?
Could you do this with Boletus Edulis. To me Edulis are by far the best Boletus.
Anxious to read about your knowledges, your suggestions.
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Speeker

Registered: 02/11/04
Posts: 877
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5928449 - 08/03/06 12:26 PM (17 years, 5 months ago) |
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yeah! it would be truly amazing if i could get results like that..  no, it's from some Japanese scientific journal..
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Feelers
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5929499 - 08/03/06 07:13 PM (17 years, 5 months ago) |
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Quote:
Yamanaka, K., Namba, K. and Tajiri, A. (2000) Fruit body formation of Boletus rericularus in pure culture. Mycoscience, 41, 189.191.
Is the paper this is from??? I found another good one - well the abstract looks good. Gotta figure out how to get it...
Quote:
Edible ectomycorrhizal mushrooms: challenges and achievements, Wang Yun and Ian R. Hall, Can. J. Bot. 82(8): 1063–1073 (2004)
Edited by Feelers (08/03/06 07:19 PM)
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Feelers
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Re: Boletus edulis (King Boletes) [Re: Feelers]
#5929584 - 08/03/06 07:39 PM (17 years, 5 months ago) |
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Log in to view attachment
Here's the second one...^^^^^
Quote:
Edible ectomycorrhizal mushrooms: challenges and achievements, Wang Yun; Ian R Hall Canadian Journal of Botany; Aug 2004; 82, 8; Academic Research Library pg. 1063

It's in French too! Micololo Edit Sorry only the abstracts in French
Edited by Feelers (08/03/06 07:47 PM)
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Speeker

Registered: 02/11/04
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Re: Boletus edulis (King Boletes) [Re: Feelers]
#5930397 - 08/04/06 02:10 AM (17 years, 5 months ago) |
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Quote:
Feelers said: Is the paper this is from???
probably that's the journal.. you'll have to find it out youself.. 
http://translate.google.com/translate?hl...noko/mokuji.htm
nice collection of articles (or chapters) there - only in Japanese, though..
this site is interesting too..
Mycorrhiza Literature Exchange
Edited by Speeker (08/04/06 02:23 AM)
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5932363 - 08/04/06 06:36 PM (17 years, 5 months ago) |
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Guys you're amazing to find good articles on the net. Thanks, I'm far to be as skillful as you are.
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Interested
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Re: Boletus edulis (King Boletes) [Re: Feelers]
#5932415 - 08/04/06 07:03 PM (17 years, 5 months ago) |
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I've uploaded the journal article here: http://rapidshare.de/files/28225453/14422576.pdf.html
Someone might want to mirror it; I'm unsure how long it'll stay up there.
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Workman
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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5933411 - 08/05/06 12:33 AM (17 years, 5 months ago) |
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Those mushrooms growing from the bottles do not look like Boletus to me, more like some sort of polypore similar to Boletopsis. Many polypores used to be in the Boletus genus but have long been moved into various other genera. Perhaps the Japanese journal is using an old designation.
-------------------- Research funded by the patrons of The Spore Works Exotic Spore Supply My Instagram Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Speeker

Registered: 02/11/04
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Re: Boletus edulis (King Boletes) [Re: Workman]
#5933647 - 08/05/06 01:57 AM (17 years, 5 months ago) |
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who needs scientists when we have workmen around...  yeah, as i said, they do look a bit peculiar..
managed to figure out how to make google translate that pdf.. 
That bolete chapter.. http://translate.google.com/translate?hl...inoko/2-2-7.pdf
and abstract of that Mycoscience article mentioned there.. http://translate.google.com/translate?hl..._abst_44_4.html
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Zen Peddler


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Re: Boletus edulis (King Boletes) [Re: Speeker]
#5936302 - 08/06/06 06:45 AM (17 years, 5 months ago) |
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great thread. Keep up the good work and research
--------------------
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Zen Peddler]
#5941976 - 08/07/06 07:54 PM (17 years, 5 months ago) |
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I now have managed a new square in the plantation for another experience for Boletus Edulis (like mentioned in the document I've post earlier). And I have another well for working, wishing I can get more and more King Boletes. It says in the document that the quantity of the mushrooms harvested depend on the habilities and quality of the mycelium a year before.
Edited by micololo2 (08/07/06 07:55 PM)
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Corporal Kielbasa

Registered: 05/29/04
Posts: 17,235
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5943559 - 08/08/06 09:59 AM (17 years, 5 months ago) |
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What mushrooms have already grown in the area? Sometimes one mushroom will grow for years in an area till it took all it could use. Then another mushroom with different needs comes in and says hey this spot is great.....
well thats just 1 of a 1000 small variables....
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micololo2
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Lactarius, Boletus, Amanitas. A well will give avalable water, it's a lot more than just 1 of 1000 small variables. Have a good day carporal and thanks to your interest.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5982374 - 08/20/06 09:01 PM (17 years, 5 months ago) |
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Now my new king bolete slush.

As you know I'm working on trying to make somekind of simple inoculation way for this specie I love. The jars you see have made from different or same parts of the mushrooms. I expcect that the slushes appearences will change a lot in the next 24-36 hours or so. I will multiplicate in different gages (10%,15%,20% etc. inoculation rate in new bottles) all the different mixes of the slushes. And in different timings and in not more than 7 days. I'll do different ways of innoculations on the experiment area. Management of the entry of light in the canopy is important for this specie. Oh yes I know I might be crazy, it looks to simple to be real... Like growing shiitake, eryngii in a trench in an open field or yellow oyster in the kitchen or maitake near the entry door or pounds and pounds of lion's mane in 100$ growing rooms.
Later +
Edited by micololo2 (08/20/06 09:08 PM)
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5985987 - 08/22/06 06:53 AM (17 years, 5 months ago) |
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The slushes will be apply today . I'll install paper pads under the rootless to keep them sandwiched between the top earth and the slush.
What do you think about this experience? You have an idea?
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Corporal Kielbasa

Registered: 05/29/04
Posts: 17,235
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5986063 - 08/22/06 08:15 AM (17 years, 5 months ago) |
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I dont understand your method of application. Could you please explain how you will go about planting them with paper and earth and the slush? Oh and why is the fourth jar from the left so dark?
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micololo2
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Quote:
Corporal Kielbasa said: I dont understand your method of application. Could you please explain how you will go about planting them with paper and earth and the slush? Oh and why is the fourth jar from the left so dark?
All the jars have something different because they've being made with different parts of the mushrooms and with different ages mushrooms.
Here 3 ways I have inoculated rootless on 20 years old white spruces.
With the pad under the rootless.

Rootless sandwiched in the inoculation pad

Slush on the rootless whitout any pad.

The importance of managing the canopy of the forest to get more shrooms. The sunlight has to penetrate it. Having a luxmeter to set it helps a lot.

Later+
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Corporal Kielbasa

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Re: Boletus edulis (King Boletes) [Re: micololo2]
#5986958 - 08/22/06 03:36 PM (17 years, 5 months ago) |
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Ok thank you for explaining.
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#6021576 - 09/02/06 07:21 AM (17 years, 4 months ago) |
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At about the 25th attempt I got for the first time this white mycelium from a very young King Boletus, less then an 1 inch tall. The 2 looking white balls are the places where the pieces of the mushroom were placed in the plate. They were the size of a rice grain. The agar was made with brewer's yeast + semolina corn and corn syrop at PH 5.
The mushroom was soak in H2O2 for 10 seconds in steril environment and then washed with alcool 90%. After that the mush. was cut in 2 pieces. The little pieces for inoculation were taking from the junction of the stem and the hat in formation.
I've made 8 slants of them and will try to inoculate rootless next spring.
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Kerbouchard
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#6056045 - 09/13/06 02:20 AM (17 years, 4 months ago) |
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Amazing!
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micololo2
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Re: Boletus edulis (King Boletes) [Re: Kerbouchard]
#6057562 - 09/13/06 03:38 PM (17 years, 4 months ago) |
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Thanks to your interest Flowda.
Would be very amazing having mushrooms right away in fall 2007 from the places that have been inoculated. In the same time I'm working on a similar project with Chanterelles. I can tell you, got to be patient with these mushrooms.
In the same time would be interesting having another grower trying something similar to compare the results. I still looking to find a way to inoculate those mush. with some production.
Have a good day both
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micololo2
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Re: Boletus edulis (King Boletes) [Re: micololo2]
#6096534 - 09/24/06 07:02 PM (17 years, 4 months ago) |
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I've harvested 12 Pd's of them today, and looking for more tomorrow.
But look it that Freesporeprint, exactly like my logo company. Incredible, what a coincidence, isn't it. Can you make something with it?
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