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OfflinePowRGnome
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Registered: 04/01/05
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Dry WBS + buttload of LC = Fast Jars?
    #4913616 - 11/09/05 02:00 PM (18 years, 3 months ago)

Hey, all. Great resource you got here. Every time I think about asking a question I just hit the old search button and bam! Got what I'm looking for.

I haven't had much success with cakes, but I think I've learned all my lessons (incubation temp, gas exchange, not coughing on the jars, the list goes on...) and I'm trying to do a bulk grow. I've started with some 2% malt/2% dextrose liquid culture and it seems to be coming along pretty well. My plan is to nocc up some quarts of WBS with it.

I've seen a couple of people mention leaving the WBS jars a little dry and using a whole lot of LC. I was wondering how you figure the math for this. It seems to me the easiest way to do it would be to soak/simmer a certain amount, then add some more dry depending how much LC you're using? Is that cool, or does the soak/simmer process get rid of some nasty stuff? If you don't do this, how do you leave it dry? Squeeze it a little harder?

Any help with this would be appreciated, especially if someone has a formula already.

Thanks, guys and girls! This forum kicks ass.


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Invisibleagar
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Registered: 11/21/04
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: PowRGnome]
    #4913692 - 11/09/05 02:20 PM (18 years, 3 months ago)

If you are doing QUART jars of WBS?


Simply soak it 24 to 36 hours.
READ THIS ABOUT 'SOAKING WBS"

Then rinse WELL.

THEN LEAVE IT OUT TO DRY - FOR like 2 HOURS, and once in awhile shake it around, to insure it is all getting dryer.

Normal spore innoc into a quart jar is close to 2 ml.

Go with 4 to 6 ml of LC.
One squirt down the center, & one squirt in each corner of the jar.  :thumbup:

After innoc, don't shake the jars.
Wait until you have about 15 / 20% colonization.



Then shake ONCE.


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OfflineCray
I'm not fat i'mbig bones
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: agar]
    #4913725 - 11/09/05 02:27 PM (18 years, 3 months ago)

agar's the WBS king...


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Invisibleagar
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: Cray]
    #4913738 - 11/09/05 02:31 PM (18 years, 3 months ago)

Quote:

Cray said:
agar's the WBS king...




Nope. agar is just FRUGAL.
WBS as SPAWN gives you the most BANG for the buck.

PETCO....BIRD BASIC BRAND
40 lb bag @ $8 each.


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Offlineblackout
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: agar]
    #4916479 - 11/10/05 02:29 AM (18 years, 3 months ago)

The math is easy. Get the recipie you want say 100g grain to 100ml water. Then add 70ml water instead. Then add 30ml of LC. I did this with wheat grain and ended up with this after 3 days


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OfflinePowRGnome
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Registered: 04/01/05
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: blackout]
    #4917167 - 11/10/05 09:33 AM (18 years, 3 months ago)

Hey thanks guys. I was mostly planning on following Doc's WBS tek, and I'm not so sure how to incorporate blackout's grain/water ratio. It seems most WBS teks I've read, including agar's final word on soaking, don't rely on any ratio. However, one can't argue with results, and three days to those kind of results....makes my mouth water.

So, blackout, I was wondering if you do in fact soak, and if so, how you still use this ratio? The two methods I've extracted from your and agar's responses (agar: soak, you: measure and mix) just seem kind of at odds, unless I've missed something about your tek.

Thanks a lot.


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Offlineblackout
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: PowRGnome]
    #4917256 - 11/10/05 09:53 AM (18 years, 3 months ago)

Ok you can soak if you use a scales. Agar is saying to soak for 24hrs or 36hours. You could do a tester with say 10g of grain and soak for 36hours. now weigh it, say it is 20g. So it has absorbed 1ml of water per 1g grain in 36 hours. You will want to add less so you can add more LC. So soak 100g for maybe 12-18hours. Now if you weigh the grain it may be only 170g (it would have been 200g with a full 36hour soak) so you can add 30ml of LC to it to bring it up to 200g.


Edited by blackout (11/10/05 09:54 AM)


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OfflinePowRGnome
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Registered: 04/01/05
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Re: Dry WBS + buttload of LC = Fast Jars? [Re: blackout]
    #4917295 - 11/10/05 10:09 AM (18 years, 3 months ago)

Oh, word. So figure out how much it can really absorb, soak it to a known weight that's less than that, and then add LC to make up the difference. Pretty slick, thanks. Do you not soak, black? You just measure and mix?


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