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OfflineOregonBluesGil
Forager/Gatherer
Male User Gallery

Registered: 09/22/04
Posts: 367
Loc: Humboldt County
Last seen: 2 hours, 5 minutes
Re: new pics and serious question [Re: Bi0TeK]
    #4917276 - 11/10/05 09:59 AM (18 years, 4 months ago)

Hello I have some Nor-cal Cyan spore Prints,whats the Best way to get started with just spores


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I'm in a Magical Mushroom land!

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OfflineSilverwolf
sandtrout
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Registered: 09/06/03
Posts: 1,108
Loc: Darkover
Last seen: 13 years, 4 months
Re: new pics and serious question [Re: OregonBluesGil]
    #4918142 - 11/10/05 01:30 PM (18 years, 4 months ago)

When you remove the mycellium do you simply remove the chips the fruiting bodies are on or do you need to go below the surface ? Once removed how quickly should the transplant be completed and what is the storage method whilst in transit; i.e plastic or non-plastic,bag or box, filtered or air-tight ? Would you line the hole with pre-soaked alder chips and how shallow is "shallow" -metric or imperial either will do-?


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"Odrade read the word silently and then aloud.
"Arafel."
She knew this word.Reverend Mothers of the tyrants time had impressed it into the Bene Gesserit consciousness,tracing it's roots to the most ancient sources.
"Arafel:the cloud darkness at the end of the universe.""

Edited by Silverwolf (11/11/05 07:02 PM)

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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: new pics and serious question [Re: OregonBluesGil]
    #4918377 - 11/10/05 02:33 PM (18 years, 4 months ago)

Quote:

OregonBluesGil said:
Hello I have some Nor-cal Cyan spore Prints,whats the Best way to get started with just spores




Well if the spore print was taken from a wild mushroom the chances are that the print is contaminated.

To make a clean culture I pasteurize a small square of cardboard (wood-lovers devour cardboard) and transfer it to antibiotic or peroxidated agar before inoculating the cardboard with spores. When the spores germinate they colonize the cardboard before then spreading onto the agar.

I then transfer wedges of the most vigorous healthy mycelium to fresh antibiotic agar and once they have colonized transfer more wedges of healthy mycelium. You basically just do this again and again until you have a clean culture.

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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.

Edited by Bi0TeK (11/10/05 02:41 PM)

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Offlineoregonshroomer
Stranger
Registered: 10/29/05
Posts: 18
Last seen: 17 years, 3 months
Re: new pics and serious question [Re: Bi0TeK]
    #4921404 - 11/11/05 08:29 AM (18 years, 4 months ago)

When you say pasturized, what do you mean.

And where can I find a suitable agar. or what type of agar do you use

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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: new pics and serious question [Re: oregonshroomer]
    #4921662 - 11/11/05 10:05 AM (18 years, 4 months ago)

Quote:

oregonshroomer said:
When you say pasturized, what do you mean.




pasteurize

v.t. sterilize partially, by heating to, and maintaining at, a high temperature. pasteurization, n.

Quote:

oregonshroomer said:
And where can I find a suitable agar. or what type of agar do you use




Many of the shroomery sponsors sell premixed agar ( Sporeworks for example) but I've always made my own by purchasing inexpensive agar agar from Asian supermarkets and adding either malt extract (MEA) or potato, dextrose and yeast (PDYA).

Adding hydrogen peroxide (H202) to the mix will kill bacteria and fungal spores and inhibit the growth of yeasts. Unfortunately mushroom spores are killed on contact with the peroxidated agar but this is where the pasteurized cardboard square comes into effect. The cardboard acts as a barrier allowing the spores to germinate before colonizing the peroxidated agar.

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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.

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Offlineoregonshroomer
Stranger
Registered: 10/29/05
Posts: 18
Last seen: 17 years, 3 months
Re: new pics and serious question [Re: Bi0TeK]
    #4922605 - 11/11/05 02:56 PM (18 years, 4 months ago)

So how do you pasturize the cardboard square?

Do most asian supermarkets carry agar. Is there a special kind to look for? HOw do you add that other stuf to your agar. Just pour the peroxide on the agar.

And if im right you were saying to place the pasturized cardboard piece in the middle of the agar and then spore print it.

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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: new pics and serious question [Re: oregonshroomer]
    #4922671 - 11/11/05 03:15 PM (18 years, 4 months ago)

The answers to the majority of your questions can be found here

http://www.shroomery.org/index/par/3

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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.

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Offlineoregonshroomer
Stranger
Registered: 10/29/05
Posts: 18
Last seen: 17 years, 3 months
Re: new pics and serious question [Re: Bi0TeK]
    #4922740 - 11/11/05 03:33 PM (18 years, 4 months ago)

Thanks

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