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Amazon Shop: ½ Pint Jars, Vermiculite, Wild Bird Seed

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OfflineMklangelo
enthusiast
Registered: 10/31/01
Posts: 297
Loc: Continental United States
Last seen: 15 years, 7 months
5 days, no sign of mycellial growth...
    #490855 - 12/14/01 10:47 AM (15 years, 9 months ago)

Finch seed/verm 80/20. 3cc's from spore syringe on 12-8. It's been in the proper inncubation environment. Substrate may be a bit on the dry side.



Any thoughts?


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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 12 years, 1 month
Re: 5 days, no sign of mycellial growth... [Re: Mklangelo]
    #490857 - 12/14/01 10:55 AM (15 years, 9 months ago)

Wait, I had to wait 7 days on my first jars to see myc.
Patience, patience...PATIENCE!
ta
lem


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OfflineScourge
The Dr.
Registered: 12/04/00
Posts: 293
Loc: Near flint Michgan, USA
Last seen: 11 years, 9 months
Re: 5 days, no sign of mycellial growth... [Re: Mklangelo]
    #490858 - 12/14/01 10:59 AM (15 years, 9 months ago)

What tec are you using? Quart jars I assume because I didn't see a verm barrier. Explain your process but for now my best gues is contamination. How long you pressure cook it for?


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OfflineMklangelo
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Registered: 10/31/01
Posts: 297
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Re: 5 days, no sign of mycellial growth... [Re: lemming]
    #490862 - 12/14/01 11:07 AM (15 years, 9 months ago)

right-y-o


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OfflineMklangelo
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Registered: 10/31/01
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Last seen: 15 years, 7 months
Re: 5 days, no sign of mycellial growth... [Re: Scourge]
    #490870 - 12/14/01 11:12 AM (15 years, 9 months ago)


The tek is steeped Finch seed for 40 min, approx 20% verm added, PC'd @ around 15 psi for 50 min. (I think that's what he said he did) knocked up the next day using the oven door method. And yes they are quart jars.

They are bathing in a mixture of H2O/H2O2 @ 86deg(F) in complete darkness.


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OfflineScourge
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Registered: 12/04/00
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Loc: Near flint Michgan, USA
Last seen: 11 years, 9 months
Re: 5 days, no sign of mycellial growth... [Re: Mklangelo]
    #490887 - 12/14/01 11:44 AM (15 years, 9 months ago)

There's your problem right there, it's contamanated.
Finch seed needs more than 50 min at 15 psi. it needs at least 90 min. I alyways do mine near 2 hours to be safe.


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OfflineMklangelo
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Re: 5 days, no sign of mycellial growth... [Re: Scourge]
    #490910 - 12/14/01 12:06 PM (15 years, 9 months ago)

Ok. I'm not trying to argue here but what about this?


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Offlinelemming
evil

Registered: 10/28/01
Posts: 107
Last seen: 12 years, 1 month
Re: 5 days, no sign of mycellial growth... [Re: Mklangelo]
    #491012 - 12/14/01 01:48 PM (15 years, 9 months ago)

Just wait and see...
You'd probably see contams by now if there were any.
Just wait!
ta
lem


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OfflineScourge
The Dr.
Registered: 12/04/00
Posts: 293
Loc: Near flint Michgan, USA
Last seen: 11 years, 9 months
Re: 5 days, no sign of mycellial growth... [Re: Mklangelo]
    #491850 - 12/15/01 10:03 AM (15 years, 9 months ago)

in my experiance it contams with bacteria if PCed under an hour for sure.


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OfflineMklangelo
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Registered: 10/31/01
Posts: 297
Loc: Continental United States
Last seen: 15 years, 7 months
Re: 5 days, no sign of mycellial growth... [Re: Scourge]
    #491866 - 12/15/01 10:37 AM (15 years, 9 months ago)

I think he's gonna go with a different method... This time 60 min PC... No steeping. Just rinse and 2:1 water/seed in jars...


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Amazon Shop: ½ Pint Jars, Vermiculite, Wild Bird Seed

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