|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 9 months
|
Lc questions among other things
#4902587 - 11/06/05 10:39 PM (18 years, 6 months ago) |
|
|
ok i searched all over the place and cant find the questions that i am asking and if it is on here plz just post a link so i can research it and not bother you guys........ ok for lc can i just take a small piece of myc from colonizing poo and put it in the jar with malt and water ? (like a piece the size of a dime or somethin) instead of using spores? and also for wbs i have a serious problem with contams (i live in a house that was like built in 1920's or so ) can i soak the wbs and then simmer for 30 mins then pc ? or would that be too much ? i do appreciate all the answers that you guys will give me ( or a link is cool too thanks again guys)
-------------------- "Anything i say is fictional" what you should look for in manure
|
smarties
Stranger


Registered: 03/07/05
Posts: 307
Last seen: 14 years, 1 month
|
Re: Lc questions among other things [Re: xaxphaanes]
#4902792 - 11/06/05 11:25 PM (18 years, 6 months ago) |
|
|
ive read that you can use myc to make liquid cultures, havent tryed it yet though. thats how i do my wbs. soak over night, simmer for 20-30 mins, drain till it doesnt drip anymore then load up the jars and pc for 60 mins.
-------------------- you need supplies? clicky clicky
|
Rejak
Cultivator

Registered: 11/29/02
Posts: 75
Loc: Canada
Last seen: 17 years, 9 months
|
Re: Lc questions among other things [Re: xaxphaanes]
#4902830 - 11/06/05 11:38 PM (18 years, 6 months ago) |
|
|
or... steep your grain for an hour. Load your jars and PC for an hour.
If you add grain into a LC jar there is a greater chance of contamination, from posible endospore still inside the grain. But it could be fine. A better plan would be to just add a small piece of mycelium. It doesnt take much, the smallest little piece can fill a LC jar with fluffy mycelium in short order.
-------------------- Need Spores? Only 2 choices worth your time! Here or FSRC Don't forget to donate to the FSRC
|
jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
|
Re: Lc questions among other things [Re: Rejak]
#4902841 - 11/06/05 11:42 PM (18 years, 6 months ago) |
|
|
Quote:
Rejak said: If you add grain into a LC jar there is a greater chance of contamination, from posible endospore still inside the grain. But it could be fine. A better plan would be to just add a small piece of mycelium.
I beleive he said taking a piece of mycelium from colonized poo...not grain.
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
Edited by jarroddupont (11/07/05 12:52 AM)
|
xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 9 months
|
Re: Lc questions among other things [Re: jarroddupont]
#4903060 - 11/07/05 12:44 AM (18 years, 6 months ago) |
|
|
yea i used the pf tek and then spawned to poo and it is fully colonized so it isnt wbs that i want to make a lc out of just the poo and i dont really know if it will work or not but eh it is cheap i might give it a go and then post results
-------------------- "Anything i say is fictional" what you should look for in manure
|
jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
|
Re: Lc questions among other things [Re: xaxphaanes]
#4903094 - 11/07/05 12:52 AM (18 years, 6 months ago) |
|
|
To answer your question, yes it will work.
Spores, other LC, or live mycelium will inoculate an LC.
After putting a chunk of myc in there, I'd also throw in an agitator (marble or piece of glass or something) to break the mycelium up when shaken.
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
|
xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 9 months
|
Re: Lc questions among other things [Re: jarroddupont]
#4903167 - 11/07/05 01:12 AM (18 years, 6 months ago) |
|
|
ok cool thanks man i will post result i am making 3 lc's one outa malt, honey, and water with added dextrose....so i should have a ready lc in what like a week or two man this is ganna be awesome and thanks again mate
-------------------- "Anything i say is fictional" what you should look for in manure
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Lc questions among other things [Re: xaxphaanes]
#4903506 - 11/07/05 06:20 AM (18 years, 6 months ago) |
|
|
LC's are very prone to contamination, if ever exposed to open air.
You can introduce a piece of mycelium tissue into a contained LC, in order to create a large liquid culture. However, the tissue must be clean & the procedure done in very aseptic conditions - like a glove box. Otherwise - when you open the LC to place the tissue inside the container, the LC often also becomes contaminated.
Best procedure I know is to use a BIOPSY needle to extract clean tissue from the inside of a mushroom stem, then inject that tissue into an LC. That procedure eliminates most exposure to open air.
--------------------
|
blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 6 months, 11 days
|
Re: Lc questions among other things [Re: agar]
#4903602 - 11/07/05 07:55 AM (18 years, 6 months ago) |
|
|
Yes, I have done it with spore syringe needles. Get a thick stem and wipe its sides with alcohol/bleach or cut it, then pass the needle all the way through it, it cuts out a core of stem. Make sure the syringe has air/water in it so you can inject it into your LC into a self healing silicone port.
|
Prajna
ReliablyUnreliable


Registered: 10/08/05
Posts: 588
Loc: Proud Canadian
Last seen: 18 years, 1 month
|
Re: Lc questions among other things [Re: blackout]
#4906542 - 11/07/05 09:06 PM (18 years, 6 months ago) |
|
|
Where do you guys find these needles?
|
agar
old hand


Registered: 11/21/04
Posts: 9,056
Loc: Somewhere Else
|
Re: Lc questions among other things [Re: Prajna]
#4907090 - 11/07/05 10:17 PM (18 years, 6 months ago) |
|
|
Best bet for local finds is feed & seed stores that cater to farmers, horsemen & cattle / cows. 10 ml / luer lock / 18 gauge / 1.5 inch needle.
I think a couple vendors here - sell them - also.
On line -- there are many sources.
--------------------
|
Rejak
Cultivator

Registered: 11/29/02
Posts: 75
Loc: Canada
Last seen: 17 years, 9 months
|
Re: Lc questions among other things [Re: agar]
#4909203 - 11/08/05 01:19 PM (18 years, 6 months ago) |
|
|
Those same feed stores could also have the needles in 14 gauge as well. The 14 gauge are great for liquid culture syringes, and biopsy.
-------------------- Need Spores? Only 2 choices worth your time! Here or FSRC Don't forget to donate to the FSRC
|
caboomers
Stranger
Registered: 07/11/05
Posts: 157
Last seen: 15 years, 3 months
|
Re: Lc questions among other things [Re: Rejak]
#4914130 - 11/09/05 03:59 PM (18 years, 6 months ago) |
|
|
just take some boiled water,put it in a baby food jare( with hole and foil on the lid after youve steamed it a while), about 2wice the amount of water for one syring,(let the water cool of course) take a chunk of myc with steril spoon (ive used my hands w/gloves) put it in jar, shake with ur finger over the hole (remember where it is), extract with sterile syring from the hole in the lid of the jar. and yes i would suggest an agitaor liek glass, ive had nothin but success, use the oven also and try to get everything as steril as possible, i would think poo would make the water fairly dark tho and you cant see how much myc ur gettin in but i could be wrong
Edited by caboomers (11/09/05 04:01 PM)
|
caboomers
Stranger
Registered: 07/11/05
Posts: 157
Last seen: 15 years, 3 months
|
Re: Lc questions among other things [Re: caboomers]
#4914131 - 11/09/05 04:00 PM (18 years, 6 months ago) |
|
|
and how do i get my thing off stranger, its so annoying
|
caboomers
Stranger
Registered: 07/11/05
Posts: 157
Last seen: 15 years, 3 months
|
Re: Lc questions among other things [Re: caboomers]
#4914153 - 11/09/05 04:05 PM (18 years, 6 months ago) |
|
|
i bet these would work great, getting tons of myc in, and would allow nice chunks to inocc tons of jars and bags, i have a couple i just havent tried them yet but i will soon and let ya know
|
xaxphaanes
Mycologist


Registered: 08/08/05
Posts: 2,988
Last seen: 1 year, 9 months
|
Re: Lc questions among other things [Re: caboomers]
#4916453 - 11/10/05 02:12 AM (18 years, 6 months ago) |
|
|
wow that is awesome i would have never of thought to do that i will try that since it sounds pretty freakin simple and i will post results if it does work with poo ty
-------------------- "Anything i say is fictional" what you should look for in manure
|
jarroddupont
I shroom we Allshroom

Registered: 10/02/05
Posts: 2,106
Loc: midwest of ...
|
Re: Lc questions among other things [Re: xaxphaanes]
#4916460 - 11/10/05 02:16 AM (18 years, 6 months ago) |
|
|
Hella Simple, hard to mess up really..
GL and have fun with that poo
-------------------- P. Cubensis Growth Parameters "All mushrooms are edible, but some only once." -- Croatian Proverb
|
|