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Invisibledjveiox
newbie
Registered: 09/19/01
Posts: 35
Loc: Troms?, Norway
Using Agar medium directly from spore print??
    #481893 - 12/06/01 01:53 PM (19 years, 10 months ago)

I have a perfect spore print, and a bag of agar, all I need is a petri dish.. Is it possible to directly use a Gulf Coast spore print, and skip the syringe making??? I cant find syringes anywhere in my town and someone i know is eager to start another batch ASAP.. please respond asap as the mail is going out to Spore Trading Post for a trade for a B+ syringe.


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OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,339
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 1 month, 2 days
Re: Using Agar medium directly from spore print?? [Re: djveiox]
    #481920 - 12/06/01 02:23 PM (19 years, 10 months ago)

Multispore innoculation on Agar.
Yes, it can be done. I would use a glovebox though.
Have you choose a recipe for your agar mixture ?

Peace,
MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Invisibledjveiox
newbie
Registered: 09/19/01
Posts: 35
Loc: Troms?, Norway
Re: Using Agar medium directly from spore print?? [Re: MAIA]
    #481930 - 12/06/01 02:34 PM (19 years, 10 months ago)

where can i find info on Multispore innoculation on Agar?? I did a search on the www board here, and on shroomery.org.. nothing.. thanks man!


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Offlinefelixhigh
KIA
Male User Gallery

Registered: 06/24/01
Posts: 7,543
Loc: Ly
Last seen: 2 years, 1 month
Re: Using Agar medium directly from spore print?? [Re: djveiox] * 1
    #482081 - 12/06/01 04:40 PM (19 years, 10 months ago)

i think you need more than a petri dish, agar and a spore print in order to be successful. first you need to know exactly what you're doing. the mushroom cultivator and other books can help you more than teks on the web. second you'll need a refrigerator where you can store media dishes/slants, potatoes, dextrose, yeast, malt extract, scalpel, _several_ petri dishes, gloves, mask and maybe some antibiotics. not to mention the whole sterile lab/closet if possible, a flow hood or glovebox can be useful if you cant make up a lab.
my wookie friend would use the pf/mmgg tek if i were in this persons place


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Invisibledownforpot
Stranger
Male
Registered: 06/25/01
Posts: 5,715
Re: Using Agar medium directly from spore print?? [Re: felixhigh]
    #482112 - 12/06/01 05:07 PM (19 years, 10 months ago)

why is it needed to mix agar? cant i just use like malt extract? also, how can i kill bacteria in agar that has some myceiium and some mold growin? anyway to kill the mold?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"


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Offlinefelixhigh
KIA
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Registered: 06/24/01
Posts: 7,543
Loc: Ly
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Re: Using Agar medium directly from spore print?? [Re: downforpot]
    #482124 - 12/06/01 05:14 PM (19 years, 10 months ago)

well, agar itself is not very nutritious for the mushrooms so you have to add some nutritive things like potato broth, dextrose or malt extract. somebody said you can make a liquid medium growth made out of water and malt extract.
about your contams, if they're already set i think you should throw it out and start another batch (whatever you're doing agar or liquid media) with some gentamicyn and/or H2O2. dont forget that h2o2 kills spores, works only with mycelia.


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Invisibledownforpot
Stranger
Male
Registered: 06/25/01
Posts: 5,715
Re: Using Agar medium directly from spore print?? [Re: felixhigh]
    #482138 - 12/06/01 05:21 PM (19 years, 10 months ago)

can i just buy malt extract agar? and use it for mycelium?

also, i am going to buy petri dishes, now its 1 glass one for 4 dollars or a pack of 20 disposable for like 4.24. how do i sterelize the plastic ones? is it better to buy glass ones or just just disposables? and im gonna store mycelium in test tubes, im gonna be usin glass ones, how do i sterelize em?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"


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Invisibledownforpot
Stranger
Male
Registered: 06/25/01
Posts: 5,715
Re: Using Agar medium directly from spore print?? [Re: downforpot]
    #482218 - 12/06/01 06:41 PM (19 years, 10 months ago)

can i use a premixed agar recipe of Malt Extract Agar? or do i gotta buy all that other shit and mix with agar agar?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"


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Invisibledimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
Re: Using Agar medium directly from spore print?? [Re: downforpot]
    #482245 - 12/06/01 07:33 PM (19 years, 10 months ago)

can i use a premixed agar recipe of Malt Extract Agar?

Yes you can adding other stuff just adds to the nutritional value of your MEA and helps make mycelium healthier.

Has anyone told this guy about the spore trading post??

That guy is a thief and a moron and I would sugest that you avoid that site at all cost's


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OfflineTamrylin
member
Registered: 08/18/01
Posts: 170
Loc: USA, East Coast
Last seen: 19 years, 2 months
Re: Using Agar medium directly from spore print?? [Re: felixhigh]
    #482252 - 12/06/01 07:39 PM (19 years, 10 months ago)

i think i heard this somewhere: would brown sugar be an acceptable substitiute for malt extract?


--------------------
Tangerine trees and marmalade skies...


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Invisibledimitri211
Carpal Tunnel
Registered: 04/27/01
Posts: 2,248
Re: Using Agar medium directly from spore print?? [Re: Tamrylin]
    #482256 - 12/06/01 07:43 PM (19 years, 10 months ago)

Why not just use Malt extract


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OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,339
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 1 month, 2 days
Re: Using Agar medium directly from spore print?? [Re: dimitri211]
    #482745 - 12/07/01 06:15 AM (19 years, 10 months ago)

Honey and agar works, you have to be very sterile though.

Peace,
MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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OfflineBeppoMarx
old hand
Registered: 09/01/01
Posts: 1,126
Last seen: 16 years, 6 months
Re: Using Agar medium directly from spore print?? [Re: MAIA]
    #482958 - 12/07/01 12:30 PM (19 years, 10 months ago)

i would assume karo corn syrup works also. i heard someone talk about that in mycellium water and that its 95% dextrose and its also clear so you can see spores germinating rather than with honey where its more difficult. Ive made 2 corn syrup/myc water 1/2 pints and theyre both coming along well enough; so I would assume on an agar plate it would do the same assuming it was mixed properly.


--------------------
Holy shit people; COMMON SENSE! we were all born with it where did it go?
maybe theres a tek out there to explain how to use it!
BUCKETS BRIGADE left hand man!!!


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OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,339
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 1 month, 2 days
Re: Using Agar medium directly from spore print?? [Re: BeppoMarx]
    #483805 - 12/08/01 06:55 AM (19 years, 10 months ago)

"so I would assume on an agar plate it would do the same assuming it was mixed properly"

That's exactlly the idea.

Peace,
MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


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Invisibleblahblahblah
Mad Scientist

Registered: 10/25/01
Posts: 1,022
Loc: South America
Re: Using Agar medium directly from spore print?? [Re: MAIA]
    #484061 - 12/08/01 02:47 PM (19 years, 10 months ago)

I have been wondering laterly, would malted milk work as malt extract? I know they arent the same thing but they are both malt right. Not for MEA but for like malt water. I have a ton of this stuff here at my house.


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